Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components
Autor(a) principal: | |
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Data de Publicação: | 1992 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.1111/j.1432-1033.1992.tb16694.x |
Resumo: | NIGMS NIH HHS (GM 32187; GM 414821) |
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7160 |
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Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme componentsBiochemistryNIGMS NIH HHS (GM 32187; GM 414821)Mössbauer spectroscopy was used to study the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743). Samples with different degrees of reduction were prepared using a redoxtitration technique. In the reduced cytochrome c3, all four hemes are reduced and exhibit diamagnetic Mössbauer spectra typical for low‐spin ferrous hemes (S= 0). In the oxidized protein, the hemes are low‐spin ferric (S= 1/2) and exhibit overlapping magnetic Mössbauer spectra. A method of differential spectroscopy was applied to deconvolute the four overlapping heme spectra and a crystal‐field model was used for data analysis. Characteristic Mössbauer spectral components for each heme group are obtained. Hyperfine and crystal‐field parameters for all four hemes are determined from these deconvoluted spectra.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNRavi, NatarajanMoura, IsabelCosta, CristinaTeixeira, MiguelLeGALL, JeanMoura, José J. G.HUYNH, Boi Hanh2019-09-10T22:49:47Z1992-01-011992-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4application/pdfhttps://doi.org/10.1111/j.1432-1033.1992.tb16694.xeng0014-2956PURE: 14639635http://www.scopus.com/inward/record.url?scp=0026610351&partnerID=8YFLogxKhttps://doi.org/10.1111/j.1432-1033.1992.tb16694.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:35:45Zoai:run.unl.pt:10362/80773Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:35:55.958643Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components |
title |
Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components |
spellingShingle |
Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components Ravi, Natarajan Biochemistry |
title_short |
Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components |
title_full |
Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components |
title_fullStr |
Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components |
title_full_unstemmed |
Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components |
title_sort |
Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components |
author |
Ravi, Natarajan |
author_facet |
Ravi, Natarajan Moura, Isabel Costa, Cristina Teixeira, Miguel LeGALL, Jean Moura, José J. G. HUYNH, Boi Hanh |
author_role |
author |
author2 |
Moura, Isabel Costa, Cristina Teixeira, Miguel LeGALL, Jean Moura, José J. G. HUYNH, Boi Hanh |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Instituto de Tecnologia Química e Biológica António Xavier (ITQB) RUN |
dc.contributor.author.fl_str_mv |
Ravi, Natarajan Moura, Isabel Costa, Cristina Teixeira, Miguel LeGALL, Jean Moura, José J. G. HUYNH, Boi Hanh |
dc.subject.por.fl_str_mv |
Biochemistry |
topic |
Biochemistry |
description |
NIGMS NIH HHS (GM 32187; GM 414821) |
publishDate |
1992 |
dc.date.none.fl_str_mv |
1992-01-01 1992-01-01T00:00:00Z 2019-09-10T22:49:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1111/j.1432-1033.1992.tb16694.x |
url |
https://doi.org/10.1111/j.1432-1033.1992.tb16694.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0014-2956 PURE: 14639635 http://www.scopus.com/inward/record.url?scp=0026610351&partnerID=8YFLogxK https://doi.org/10.1111/j.1432-1033.1992.tb16694.x |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4 application/pdf |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137979652898816 |