Influence of moderate electric fields in β-lactoglobulin thermal unfolding and interactions

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Rui M.
Data de Publicação: 2020
Outros Autores: Avelar, Zita, Vicente, A. A., Petersen, Steffen B., Pereira, Ricardo N.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/61336
Resumo: Supplementary data to this article can be found online at https:// doi.org/10.1016/j.foodchem.2019.125442.
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spelling Influence of moderate electric fields in β-lactoglobulin thermal unfolding and interactionsß-LactoglobulinModerate electric fieldsThermal denaturationProteins structureProtein interactionsβ-LactoglobulinScience & TechnologySupplementary data to this article can be found online at https:// doi.org/10.1016/j.foodchem.2019.125442.In this study, the effects of moderate electric fields during thermal denaturation of -lactoglobulin were examined through an in situ circular dichroism approach, complemented by intrinsic extrinsic fluorescence analysis. Results have shown that the effects of electric fields in protein unfolding were linearly dependent on the applied electric field intensity (V/cm) and increased by the use of low electric frequencies i.e. 50 to 200Hz. These electric effects caused significant changes on -lactoglobulin melting temperature, unfolded conformation and subsequent intermolecular interactions, revealed by the increase of surface hydrophobicity (ANS affinity) and higher conservation of retinol binding. The obtained data provides a clear evidence that moderate electric fields contribute to distinct folding/unfolding of -lactoglobulin, resulting in structural modifications. These findings are relevant for (bio)-technological applications involving electric fields processing, bringing new insights for the development of innovative strategies to control protein function and tune production of functional protein systems.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the scope of Norte2020, Programa Operacional Regional do Norte.Rui M.Rodrigues and Ricardo N. Pereira gratefully acknowledge FCT for their financial grants with references SFRH/BD/110723/2015 and SFRH/BPD/ 81887/2011, respectively. The authors would also like to acknowledge Pedro Silva for his contribution to this work.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoRodrigues, Rui M.Avelar, ZitaVicente, A. A.Petersen, Steffen B.Pereira, Ricardo N.20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/61336engRodrigues, Rui M.; Avelar, Zita; Vicente, António A.; Petersen, Steffen B.; Pereira, Ricardo N., Influence of moderate electric fields in β-lactoglobulin thermal unfolding and interactions. Food Chemistry, 304(125442), 20200308-814610.1016/j.foodchem.2019.12544231491714http://www.elsevier.com/locate/issn/03088146info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:03:33Zoai:repositorium.sdum.uminho.pt:1822/61336Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:53:41.971924Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Influence of moderate electric fields in β-lactoglobulin thermal unfolding and interactions
title Influence of moderate electric fields in β-lactoglobulin thermal unfolding and interactions
spellingShingle Influence of moderate electric fields in β-lactoglobulin thermal unfolding and interactions
Rodrigues, Rui M.
ß-Lactoglobulin
Moderate electric fields
Thermal denaturation
Proteins structure
Protein interactions
β-Lactoglobulin
Science & Technology
title_short Influence of moderate electric fields in β-lactoglobulin thermal unfolding and interactions
title_full Influence of moderate electric fields in β-lactoglobulin thermal unfolding and interactions
title_fullStr Influence of moderate electric fields in β-lactoglobulin thermal unfolding and interactions
title_full_unstemmed Influence of moderate electric fields in β-lactoglobulin thermal unfolding and interactions
title_sort Influence of moderate electric fields in β-lactoglobulin thermal unfolding and interactions
author Rodrigues, Rui M.
author_facet Rodrigues, Rui M.
Avelar, Zita
Vicente, A. A.
Petersen, Steffen B.
Pereira, Ricardo N.
author_role author
author2 Avelar, Zita
Vicente, A. A.
Petersen, Steffen B.
Pereira, Ricardo N.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Rodrigues, Rui M.
Avelar, Zita
Vicente, A. A.
Petersen, Steffen B.
Pereira, Ricardo N.
dc.subject.por.fl_str_mv ß-Lactoglobulin
Moderate electric fields
Thermal denaturation
Proteins structure
Protein interactions
β-Lactoglobulin
Science & Technology
topic ß-Lactoglobulin
Moderate electric fields
Thermal denaturation
Proteins structure
Protein interactions
β-Lactoglobulin
Science & Technology
description Supplementary data to this article can be found online at https:// doi.org/10.1016/j.foodchem.2019.125442.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/61336
url http://hdl.handle.net/1822/61336
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Rodrigues, Rui M.; Avelar, Zita; Vicente, António A.; Petersen, Steffen B.; Pereira, Ricardo N., Influence of moderate electric fields in β-lactoglobulin thermal unfolding and interactions. Food Chemistry, 304(125442), 2020
0308-8146
10.1016/j.foodchem.2019.125442
31491714
http://www.elsevier.com/locate/issn/03088146
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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