FLOW CYTOMETRIC EVALUATION OF PERIPHERAL BLOOD BIOMARKERS FOR SOLID TUMOURS IMMUNOTHERAPY GUIDING: A REVIEW
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.34635/rpc.852 |
Resumo: | Cancer treatment is an area that is constantly being updated, namely with the discovery of immunotherapy as a clinically effective therapeutic modality for various types of cancer. Among these innovative therapies, immune checkpoint inhibitors (ICI) have proven to provide significant and long-lasting responses. However, this response does not occur for all patients, i.e., some patients do not benefit from this therapy. Due to this heterogeneity in immunotherapy response, there is an urgent need to identify and establish biomarkers that will allow the identification of patients who will respond to the therapy, while sparing the non-responders from adverse effects. In addition, the use these biomarkers in monitoring the response during treatment seems promising. Given the recognized role of the immune system in the anti-tumour response, these cells have been intensively studied as potential biomarkers. Their study in peripheral blood (PB) has been of great interest and importance, given its easy accessibility and less invasive nature. The detailed and integral evaluation of peripheral immunity requires a multiparametric methodology such as flow cytometry (FC), applying the simultaneous analysis of lineage markers together with maturation, activation and functional state markers. In this narrative literature review, we intend to describe the “state of the art” on the FC study of PB immune cell populations as potential biomarkers for ICI therapy in solid tumours. The results found are presented for each of the major populations and their subsets, namely T lymphocytes, myeloid-derived suppressor cells (MDSCs), neutrophils, eosinophils, dendritic cells (CD), natural killer cells (NK), monocyte subsets, and B cells. |
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FLOW CYTOMETRIC EVALUATION OF PERIPHERAL BLOOD BIOMARKERS FOR SOLID TUMOURS IMMUNOTHERAPY GUIDING: A REVIEWAVALIAÇÃO DE BIOMARCADORES NO SANGUE PERIFÉRICO POR CITOMETRIA DE FLUXO COMO MEIO DE ORIENTAÇÃO PARA A IMUNOTERAPIA EM TUMORES SÓLIDOS: REVISÃO DA LITERATURACancer treatment is an area that is constantly being updated, namely with the discovery of immunotherapy as a clinically effective therapeutic modality for various types of cancer. Among these innovative therapies, immune checkpoint inhibitors (ICI) have proven to provide significant and long-lasting responses. However, this response does not occur for all patients, i.e., some patients do not benefit from this therapy. Due to this heterogeneity in immunotherapy response, there is an urgent need to identify and establish biomarkers that will allow the identification of patients who will respond to the therapy, while sparing the non-responders from adverse effects. In addition, the use these biomarkers in monitoring the response during treatment seems promising. Given the recognized role of the immune system in the anti-tumour response, these cells have been intensively studied as potential biomarkers. Their study in peripheral blood (PB) has been of great interest and importance, given its easy accessibility and less invasive nature. The detailed and integral evaluation of peripheral immunity requires a multiparametric methodology such as flow cytometry (FC), applying the simultaneous analysis of lineage markers together with maturation, activation and functional state markers. In this narrative literature review, we intend to describe the “state of the art” on the FC study of PB immune cell populations as potential biomarkers for ICI therapy in solid tumours. The results found are presented for each of the major populations and their subsets, namely T lymphocytes, myeloid-derived suppressor cells (MDSCs), neutrophils, eosinophils, dendritic cells (CD), natural killer cells (NK), monocyte subsets, and B cells. O tratamento do cancro é uma área em intensa e permanente atualização, nomeadamente com a descoberta da imunoterapia como modalidade terapêutica clinicamente eficaz para diversos tipos de cancro. Entre estas terapias inovadoras, as terapias com inibidores de checkpoints imunológicos (ICI) têm demonstrado apresentar respostas significativas e duradouras. No entanto, esta resposta não ocorre para todos os doentes, ou seja, alguns pacientes não beneficiam desta terapia. Devido a esta heterogeneidade na resposta à imunoterapia, persiste a necessidade urgente de identificar e estabelecer biomarcadores que permitam a identificação dos doentes que irão responder à terapia, poupando os que não respondem aos efeitos adversos. Para além deste aspeto, parece promissor o impacto na clínica da utilização destes biomarcadores na monitorização da resposta durante o tratamento. Dado o reconhecido papel do sistema imunológico na resposta anti-tumoral, estas células têm sido intensamente estudadas como potenciais biomarcadores. Com este objetivo, o sangue periférico (SP), tem revelado grande interesse e importância, dada a sua fácil acessibilidade e natureza menos invasiva. A avaliação detalhada e integral da imunidade periférica, exige uma metodologia multiparamétrica como a citometria de fluxo, recorrendo à utilização de marcadores de linhagem simultaneamente com marcadores de maturação, ativação e estados funcionais. Com esta revisão bibliográfica narrativa, pretendeu-se descrever o “estado da arte” sobre o estudo por citometria de fluxo das populações celulares do sistema imunológico no SP, como potenciais biomarcadores para a terapia com ICI em tumores sólidos. Os resultados encontrados são apresentados para cada uma das populações principais e suas subpopulações, nomeadamente linfócitos T, células supressoras derivadas da linhagem mieloide (MDSCs), neutrófilos, eosinófilos, células dendríticas (DC), células natural killer (NK), monócitos, e linfócitos B. Sociedade Portuguesa de Cirurgia2021-01-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.34635/rpc.852https://doi.org/10.34635/rpc.852Revista Portuguesa de Cirurgia; No 49 (2020): Number 49 - December 2020; 48-60Revista Portuguesa de Cirurgia; No 49 (2020): Number 49 - December 2020; 48-602183-11651646-6918reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttps://revista.spcir.com/index.php/spcir/article/view/852https://revista.spcir.com/index.php/spcir/article/view/852/583Copyright (c) 2021 Revista Portuguesa de Cirurgiainfo:eu-repo/semantics/openAccessTrigo, Ana CatrinaMaia, PatríciaGodinho, InêsRodrigues, Catarina A.Sousa, Maria EmíliaPires, Ana MartaAzevedo, CarlaSantos, Lúcio LaraPalmeira, CarlosMartins, Gabriela2024-03-14T22:05:38Zoai:revista.spcir.com:article/852Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T04:00:56.705685Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
FLOW CYTOMETRIC EVALUATION OF PERIPHERAL BLOOD BIOMARKERS FOR SOLID TUMOURS IMMUNOTHERAPY GUIDING: A REVIEW AVALIAÇÃO DE BIOMARCADORES NO SANGUE PERIFÉRICO POR CITOMETRIA DE FLUXO COMO MEIO DE ORIENTAÇÃO PARA A IMUNOTERAPIA EM TUMORES SÓLIDOS: REVISÃO DA LITERATURA |
title |
FLOW CYTOMETRIC EVALUATION OF PERIPHERAL BLOOD BIOMARKERS FOR SOLID TUMOURS IMMUNOTHERAPY GUIDING: A REVIEW |
spellingShingle |
FLOW CYTOMETRIC EVALUATION OF PERIPHERAL BLOOD BIOMARKERS FOR SOLID TUMOURS IMMUNOTHERAPY GUIDING: A REVIEW Trigo, Ana Catrina |
title_short |
FLOW CYTOMETRIC EVALUATION OF PERIPHERAL BLOOD BIOMARKERS FOR SOLID TUMOURS IMMUNOTHERAPY GUIDING: A REVIEW |
title_full |
FLOW CYTOMETRIC EVALUATION OF PERIPHERAL BLOOD BIOMARKERS FOR SOLID TUMOURS IMMUNOTHERAPY GUIDING: A REVIEW |
title_fullStr |
FLOW CYTOMETRIC EVALUATION OF PERIPHERAL BLOOD BIOMARKERS FOR SOLID TUMOURS IMMUNOTHERAPY GUIDING: A REVIEW |
title_full_unstemmed |
FLOW CYTOMETRIC EVALUATION OF PERIPHERAL BLOOD BIOMARKERS FOR SOLID TUMOURS IMMUNOTHERAPY GUIDING: A REVIEW |
title_sort |
FLOW CYTOMETRIC EVALUATION OF PERIPHERAL BLOOD BIOMARKERS FOR SOLID TUMOURS IMMUNOTHERAPY GUIDING: A REVIEW |
author |
Trigo, Ana Catrina |
author_facet |
Trigo, Ana Catrina Maia, Patrícia Godinho, Inês Rodrigues, Catarina A. Sousa, Maria Emília Pires, Ana Marta Azevedo, Carla Santos, Lúcio Lara Palmeira, Carlos Martins, Gabriela |
author_role |
author |
author2 |
Maia, Patrícia Godinho, Inês Rodrigues, Catarina A. Sousa, Maria Emília Pires, Ana Marta Azevedo, Carla Santos, Lúcio Lara Palmeira, Carlos Martins, Gabriela |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Trigo, Ana Catrina Maia, Patrícia Godinho, Inês Rodrigues, Catarina A. Sousa, Maria Emília Pires, Ana Marta Azevedo, Carla Santos, Lúcio Lara Palmeira, Carlos Martins, Gabriela |
description |
Cancer treatment is an area that is constantly being updated, namely with the discovery of immunotherapy as a clinically effective therapeutic modality for various types of cancer. Among these innovative therapies, immune checkpoint inhibitors (ICI) have proven to provide significant and long-lasting responses. However, this response does not occur for all patients, i.e., some patients do not benefit from this therapy. Due to this heterogeneity in immunotherapy response, there is an urgent need to identify and establish biomarkers that will allow the identification of patients who will respond to the therapy, while sparing the non-responders from adverse effects. In addition, the use these biomarkers in monitoring the response during treatment seems promising. Given the recognized role of the immune system in the anti-tumour response, these cells have been intensively studied as potential biomarkers. Their study in peripheral blood (PB) has been of great interest and importance, given its easy accessibility and less invasive nature. The detailed and integral evaluation of peripheral immunity requires a multiparametric methodology such as flow cytometry (FC), applying the simultaneous analysis of lineage markers together with maturation, activation and functional state markers. In this narrative literature review, we intend to describe the “state of the art” on the FC study of PB immune cell populations as potential biomarkers for ICI therapy in solid tumours. The results found are presented for each of the major populations and their subsets, namely T lymphocytes, myeloid-derived suppressor cells (MDSCs), neutrophils, eosinophils, dendritic cells (CD), natural killer cells (NK), monocyte subsets, and B cells. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-20 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.34635/rpc.852 https://doi.org/10.34635/rpc.852 |
url |
https://doi.org/10.34635/rpc.852 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revista.spcir.com/index.php/spcir/article/view/852 https://revista.spcir.com/index.php/spcir/article/view/852/583 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Revista Portuguesa de Cirurgia info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Revista Portuguesa de Cirurgia |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Portuguesa de Cirurgia |
publisher.none.fl_str_mv |
Sociedade Portuguesa de Cirurgia |
dc.source.none.fl_str_mv |
Revista Portuguesa de Cirurgia; No 49 (2020): Number 49 - December 2020; 48-60 Revista Portuguesa de Cirurgia; No 49 (2020): Number 49 - December 2020; 48-60 2183-1165 1646-6918 reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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