Monitoring Amphora sp. growth by flow cytometry

Detalhes bibliográficos
Autor(a) principal: Dias, Nicolina Marques
Data de Publicação: 2018
Outros Autores: Mota, D., Nicolau, Ana, Mota, M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/59447
Resumo: Supplemental data for this article can be accessed at https://doi.org/10.1080/0269249X.2018.1523231.
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spelling Monitoring Amphora sp. growth by flow cytometryautofluorescencebenthic microorganismsdiatomsflow cytometrygrowth monitoringScience & TechnologySupplemental data for this article can be accessed at https://doi.org/10.1080/0269249X.2018.1523231.A protocol on flow cytometry (FC) was developed for monitoring the growth of Amphora sp. in non-axenic and near-axenic (<15\% of bacteria) cultures, enabling the discrimination of aggregates and suspended bacterial cells. Autofluorescence emitted by diatoms was gated on the FL3 red channel and bacteria stained with SYTO-BC were detected on the FL1 green channel. In the FL3 positive region, two subpopulations were gated, one corresponding to a homogeneous high-density region, consisting mostly of single diatoms (R1), and a subpopulation of events with up to one log decade fold of FL3 fluorescence intensity and increased relative cell size, suggesting the presence of aggregates of two or more diatoms in the culture (R2). Percentages of single diatoms, diatoms with attached bacteria and free-suspended bacteria were determined in each quadrant (A, B, C) at different growth phases. The aggregates of diatoms with attached-bacterial cells increased with culture age, and the percentage of free-suspended bacteria accounted for less than 10\% in this controlled experiment. It is expected that growth monitoring by FC will allow the detection of bacterial contamination or overgrowth at early stages of Amphora sp. cultures.This study was supported by the Portuguese Science and Technology Foundation (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit, COMPETE 2020 (POCI-01-0145-FEDER-006684) and the BioTecNorte operation(NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersionInternational Society for Diatom ResearchUniversidade do MinhoDias, Nicolina MarquesMota, D.Nicolau, AnaMota, M.2018-11-202018-11-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/59447engDias, Nicolina M.; Mota, D.; Nicolau, Ana; Mota, Manuel, Monitoring Amphora sp. growth by flow cytometry. Diatom Research, 33(3), 405-411, 20180269-249X10.1080/0269249X.2018.1523231https://www.tandfonline.com/toc/tdia20/currentinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:20:51Zoai:repositorium.sdum.uminho.pt:1822/59447Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:14:00.168306Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Monitoring Amphora sp. growth by flow cytometry
title Monitoring Amphora sp. growth by flow cytometry
spellingShingle Monitoring Amphora sp. growth by flow cytometry
Dias, Nicolina Marques
autofluorescence
benthic microorganisms
diatoms
flow cytometry
growth monitoring
Science & Technology
title_short Monitoring Amphora sp. growth by flow cytometry
title_full Monitoring Amphora sp. growth by flow cytometry
title_fullStr Monitoring Amphora sp. growth by flow cytometry
title_full_unstemmed Monitoring Amphora sp. growth by flow cytometry
title_sort Monitoring Amphora sp. growth by flow cytometry
author Dias, Nicolina Marques
author_facet Dias, Nicolina Marques
Mota, D.
Nicolau, Ana
Mota, M.
author_role author
author2 Mota, D.
Nicolau, Ana
Mota, M.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Dias, Nicolina Marques
Mota, D.
Nicolau, Ana
Mota, M.
dc.subject.por.fl_str_mv autofluorescence
benthic microorganisms
diatoms
flow cytometry
growth monitoring
Science & Technology
topic autofluorescence
benthic microorganisms
diatoms
flow cytometry
growth monitoring
Science & Technology
description Supplemental data for this article can be accessed at https://doi.org/10.1080/0269249X.2018.1523231.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-20
2018-11-20T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/59447
url http://hdl.handle.net/1822/59447
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Dias, Nicolina M.; Mota, D.; Nicolau, Ana; Mota, Manuel, Monitoring Amphora sp. growth by flow cytometry. Diatom Research, 33(3), 405-411, 2018
0269-249X
10.1080/0269249X.2018.1523231
https://www.tandfonline.com/toc/tdia20/current
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv International Society for Diatom Research
publisher.none.fl_str_mv International Society for Diatom Research
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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