The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase

Detalhes bibliográficos
Autor(a) principal: Carreira, Cíntia
Data de Publicação: 2020
Outros Autores: Nunes, Rute F., Mestre, Olga, Moura, Isabel, Pauleta, Sofia R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/111967
Resumo: PTDC/BBB-BQB/0129/2014 (IM). This work was supported by the Applied Molecular Biosciences Unit-UCIBIO, and Associate Laboratory for Green Chemistry-LAQV, which is financed by national funds from FCT (UIDB/04378/2020 and UIDB/50006/2020, respectively).
id RCAP_52acf8d551e50d4997f8d67b1ec4772d
oai_identifier_str oai:run.unl.pt:10362/111967
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductaseAcidic pHDenitrificationMarine bacteriaMarinobacter hydrocarbonoclasticusNitrous oxide reductase“CuZ” centerBiochemistryInorganic ChemistrySDG 14 - Life Below WaterPTDC/BBB-BQB/0129/2014 (IM). This work was supported by the Applied Molecular Biosciences Unit-UCIBIO, and Associate Laboratory for Green Chemistry-LAQV, which is financed by national funds from FCT (UIDB/04378/2020 and UIDB/50006/2020, respectively).Abstract: Increasing atmospheric concentration of N2O has been a concern, as it is a potent greenhouse gas and promotes ozone layer destruction. In the N-cycle, release of N2O is boosted upon a drop of pH in the environment. Here, Marinobacter hydrocarbonoclasticus was grown in batch mode in the presence of nitrate, to study the effect of pH in the denitrification pathway by gene expression profiling, quantification of nitrate and nitrite, and evaluating the ability of whole cells to reduce NO and N2O. At pH 6.5, accumulation of nitrite in the medium occurs and the cells were unable to reduce N2O. In addition, the biochemical properties of N2O reductase isolated from cells grown at pH 6.5, 7.5 and 8.5 were compared for the first time. The amount of this enzyme at acidic pH was lower than that at pH 7.5 and 8.5, pinpointing to a post-transcriptional regulation, though pH did not affect gene expression of N2O reductase accessory genes. N2O reductase isolated from cells grown at pH 6.5 has its catalytic center mainly as CuZ(4Cu1S), while that from cells grown at pH 7.5 or 8.5 has it as CuZ(4Cu2S). This study evidences that an in vivo secondary level of regulation is required to maintain N2O reductase in an active state. Graphic abstract: [Figure not available: see fulltext.].LAQV@REQUIMTEUCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNCarreira, CíntiaNunes, Rute F.Mestre, OlgaMoura, IsabelPauleta, Sofia R.2021-02-16T23:30:21Z2020-10-012020-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14application/pdfhttp://hdl.handle.net/10362/111967eng0949-8257PURE: 27897711https://doi.org/10.1007/s00775-020-01812-0info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:55:44Zoai:run.unl.pt:10362/111967Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:42:02.358122Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase
title The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase
spellingShingle The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase
Carreira, Cíntia
Acidic pH
Denitrification
Marine bacteria
Marinobacter hydrocarbonoclasticus
Nitrous oxide reductase
“CuZ” center
Biochemistry
Inorganic Chemistry
SDG 14 - Life Below Water
title_short The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase
title_full The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase
title_fullStr The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase
title_full_unstemmed The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase
title_sort The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase
author Carreira, Cíntia
author_facet Carreira, Cíntia
Nunes, Rute F.
Mestre, Olga
Moura, Isabel
Pauleta, Sofia R.
author_role author
author2 Nunes, Rute F.
Mestre, Olga
Moura, Isabel
Pauleta, Sofia R.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Carreira, Cíntia
Nunes, Rute F.
Mestre, Olga
Moura, Isabel
Pauleta, Sofia R.
dc.subject.por.fl_str_mv Acidic pH
Denitrification
Marine bacteria
Marinobacter hydrocarbonoclasticus
Nitrous oxide reductase
“CuZ” center
Biochemistry
Inorganic Chemistry
SDG 14 - Life Below Water
topic Acidic pH
Denitrification
Marine bacteria
Marinobacter hydrocarbonoclasticus
Nitrous oxide reductase
“CuZ” center
Biochemistry
Inorganic Chemistry
SDG 14 - Life Below Water
description PTDC/BBB-BQB/0129/2014 (IM). This work was supported by the Applied Molecular Biosciences Unit-UCIBIO, and Associate Laboratory for Green Chemistry-LAQV, which is financed by national funds from FCT (UIDB/04378/2020 and UIDB/50006/2020, respectively).
publishDate 2020
dc.date.none.fl_str_mv 2020-10-01
2020-10-01T00:00:00Z
2021-02-16T23:30:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/111967
url http://hdl.handle.net/10362/111967
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0949-8257
PURE: 27897711
https://doi.org/10.1007/s00775-020-01812-0
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138032905879552

Registros relacionados