Genomic organization, gene expression and activity profile of Marinobacter hydrocarbonoclasticus denitrification enzymes

Detalhes bibliográficos
Autor(a) principal: Carreira, Cíntia
Data de Publicação: 2018
Outros Autores: Mestre, Olga, Nunes, Rute F., Moura, Isabel, Pauleta, Sofia R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/114130
Resumo: POCI-01-0145-FEDER-007728
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spelling Genomic organization, gene expression and activity profile of Marinobacter hydrocarbonoclasticus denitrification enzymesDenitrificationGene regulationMarinobacterNitrogen biogeochemical cycleNitrous oxideTranscriptionNeuroscience(all)Biochemistry, Genetics and Molecular Biology(all)Agricultural and Biological Sciences(all)SDG 14 - Life Below WaterPOCI-01-0145-FEDER-007728Background. Denitrification is one of the main pathways of the N-cycle, during which nitrate is converted to dinitrogen gas, in four consecutive reactions that are each catalyzed by a different metalloenzyme. One of the intermediate metabolites is nitrous oxide, which has a global warming impact greater then carbon dioxide and which atmospheric concentration has been increasing in the last years. The four denitrification enzymes have been isolated and biochemically characterized from Marinobacter hydrocarbonoclasticus in our lab. Methods. Bioinformatic analysis of the M. hydrocarbonoclasticus genome to identify the genes involved in the denitrification pathway. The relative gene expression of the gene encoding the catalytic subunits of those enzymes was analyzed during the growth under microoxic conditions. The consumption of nitrate and nitrite, and the reduction of nitric oxide and nitrous oxide by whole-cells was monitored during anoxic and microoxic growth in the presence of 10 mM sodium nitrate at pH 7.5. Results. The bioinformatic analysis shows that genes encoding the enzymes and accessory factors required for each step of the denitrification pathway are clustered together. An unusual feature is the co-existence of genes encoding a q- and a c-type nitric oxide reductase, with only the latter being transcribed at similar levels as the ones encoding the catalytic subunits of the other denitrifying enzymes, when cells are grown in the presence of nitrate under microoxic conditions. Using either a batch- or a closed system, nitrate is completely consumed in the beginning of the growth, with transient formation of nitrite, and whole-cells can reduce nitric oxide and nitrous oxide from mid-exponential phase until being collected (time-point 50 h). Discussion. M. hydrocarbonoclasticus cells can reduce nitric and nitrous oxide in vivo, indicating that the four denitrification steps are active. Gene expression profile together with promoter regions analysis indicates the involvement of a cascade regulatory mechanism triggered by FNR-type in response to low oxygen tension, with nitric oxide and nitrate as secondary effectors, through DNR and NarXL, respectively. This global characterization of the denitrification pathway of a strict marine bacterium, contributes to the understanding of the N-cycle and nitrous oxide release in marine environments.LAQV@REQUIMTEUCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNCarreira, CíntiaMestre, OlgaNunes, Rute F.Moura, IsabelPauleta, Sofia R.2021-03-19T23:22:05Z2018-01-012018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/114130eng2167-8359PURE: 6550954https://doi.org/10.7717/peerj.5603info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:56:50Zoai:run.unl.pt:10362/114130Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:42:26.942383Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genomic organization, gene expression and activity profile of Marinobacter hydrocarbonoclasticus denitrification enzymes
title Genomic organization, gene expression and activity profile of Marinobacter hydrocarbonoclasticus denitrification enzymes
spellingShingle Genomic organization, gene expression and activity profile of Marinobacter hydrocarbonoclasticus denitrification enzymes
Carreira, Cíntia
Denitrification
Gene regulation
Marinobacter
Nitrogen biogeochemical cycle
Nitrous oxide
Transcription
Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
SDG 14 - Life Below Water
title_short Genomic organization, gene expression and activity profile of Marinobacter hydrocarbonoclasticus denitrification enzymes
title_full Genomic organization, gene expression and activity profile of Marinobacter hydrocarbonoclasticus denitrification enzymes
title_fullStr Genomic organization, gene expression and activity profile of Marinobacter hydrocarbonoclasticus denitrification enzymes
title_full_unstemmed Genomic organization, gene expression and activity profile of Marinobacter hydrocarbonoclasticus denitrification enzymes
title_sort Genomic organization, gene expression and activity profile of Marinobacter hydrocarbonoclasticus denitrification enzymes
author Carreira, Cíntia
author_facet Carreira, Cíntia
Mestre, Olga
Nunes, Rute F.
Moura, Isabel
Pauleta, Sofia R.
author_role author
author2 Mestre, Olga
Nunes, Rute F.
Moura, Isabel
Pauleta, Sofia R.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Carreira, Cíntia
Mestre, Olga
Nunes, Rute F.
Moura, Isabel
Pauleta, Sofia R.
dc.subject.por.fl_str_mv Denitrification
Gene regulation
Marinobacter
Nitrogen biogeochemical cycle
Nitrous oxide
Transcription
Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
SDG 14 - Life Below Water
topic Denitrification
Gene regulation
Marinobacter
Nitrogen biogeochemical cycle
Nitrous oxide
Transcription
Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
SDG 14 - Life Below Water
description POCI-01-0145-FEDER-007728
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01
2018-01-01T00:00:00Z
2021-03-19T23:22:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/114130
url http://hdl.handle.net/10362/114130
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2167-8359
PURE: 6550954
https://doi.org/10.7717/peerj.5603
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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