The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/111967 |
Resumo: | PTDC/BBB-BQB/0129/2014 (IM). This work was supported by the Applied Molecular Biosciences Unit-UCIBIO, and Associate Laboratory for Green Chemistry-LAQV, which is financed by national funds from FCT (UIDB/04378/2020 and UIDB/50006/2020, respectively). |
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The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductaseAcidic pHDenitrificationMarine bacteriaMarinobacter hydrocarbonoclasticusNitrous oxide reductase“CuZ” centerBiochemistryInorganic ChemistrySDG 14 - Life Below WaterPTDC/BBB-BQB/0129/2014 (IM). This work was supported by the Applied Molecular Biosciences Unit-UCIBIO, and Associate Laboratory for Green Chemistry-LAQV, which is financed by national funds from FCT (UIDB/04378/2020 and UIDB/50006/2020, respectively).Abstract: Increasing atmospheric concentration of N2O has been a concern, as it is a potent greenhouse gas and promotes ozone layer destruction. In the N-cycle, release of N2O is boosted upon a drop of pH in the environment. Here, Marinobacter hydrocarbonoclasticus was grown in batch mode in the presence of nitrate, to study the effect of pH in the denitrification pathway by gene expression profiling, quantification of nitrate and nitrite, and evaluating the ability of whole cells to reduce NO and N2O. At pH 6.5, accumulation of nitrite in the medium occurs and the cells were unable to reduce N2O. In addition, the biochemical properties of N2O reductase isolated from cells grown at pH 6.5, 7.5 and 8.5 were compared for the first time. The amount of this enzyme at acidic pH was lower than that at pH 7.5 and 8.5, pinpointing to a post-transcriptional regulation, though pH did not affect gene expression of N2O reductase accessory genes. N2O reductase isolated from cells grown at pH 6.5 has its catalytic center mainly as CuZ(4Cu1S), while that from cells grown at pH 7.5 or 8.5 has it as CuZ(4Cu2S). This study evidences that an in vivo secondary level of regulation is required to maintain N2O reductase in an active state. Graphic abstract: [Figure not available: see fulltext.].LAQV@REQUIMTEUCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNCarreira, CíntiaNunes, Rute F.Mestre, OlgaMoura, IsabelPauleta, Sofia R.2021-02-16T23:30:21Z2020-10-012020-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14application/pdfhttp://hdl.handle.net/10362/111967eng0949-8257PURE: 27897711https://doi.org/10.1007/s00775-020-01812-0info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:55:44Zoai:run.unl.pt:10362/111967Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:42:02.358122Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase |
title |
The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase |
spellingShingle |
The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase Carreira, Cíntia Acidic pH Denitrification Marine bacteria Marinobacter hydrocarbonoclasticus Nitrous oxide reductase “CuZ” center Biochemistry Inorganic Chemistry SDG 14 - Life Below Water |
title_short |
The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase |
title_full |
The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase |
title_fullStr |
The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase |
title_full_unstemmed |
The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase |
title_sort |
The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase |
author |
Carreira, Cíntia |
author_facet |
Carreira, Cíntia Nunes, Rute F. Mestre, Olga Moura, Isabel Pauleta, Sofia R. |
author_role |
author |
author2 |
Nunes, Rute F. Mestre, Olga Moura, Isabel Pauleta, Sofia R. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
LAQV@REQUIMTE UCIBIO - Applied Molecular Biosciences Unit DQ - Departamento de Química RUN |
dc.contributor.author.fl_str_mv |
Carreira, Cíntia Nunes, Rute F. Mestre, Olga Moura, Isabel Pauleta, Sofia R. |
dc.subject.por.fl_str_mv |
Acidic pH Denitrification Marine bacteria Marinobacter hydrocarbonoclasticus Nitrous oxide reductase “CuZ” center Biochemistry Inorganic Chemistry SDG 14 - Life Below Water |
topic |
Acidic pH Denitrification Marine bacteria Marinobacter hydrocarbonoclasticus Nitrous oxide reductase “CuZ” center Biochemistry Inorganic Chemistry SDG 14 - Life Below Water |
description |
PTDC/BBB-BQB/0129/2014 (IM). This work was supported by the Applied Molecular Biosciences Unit-UCIBIO, and Associate Laboratory for Green Chemistry-LAQV, which is financed by national funds from FCT (UIDB/04378/2020 and UIDB/50006/2020, respectively). |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-01 2020-10-01T00:00:00Z 2021-02-16T23:30:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/111967 |
url |
http://hdl.handle.net/10362/111967 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0949-8257 PURE: 27897711 https://doi.org/10.1007/s00775-020-01812-0 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799138032905879552 |