Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model

Detalhes bibliográficos
Autor(a) principal: Casimiro, Maria Helena
Data de Publicação: 2022
Outros Autores: Ferreira, Luís M., Santos, Pedro M.P., Leal, João P., Rodrigues, Gabriela, Iria, Inês, Alves, Sara, Pais, Diogo, Casal, Diogo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/147552
Resumo: Funding Information: This research was funded by the International Atomic Energy Agency under Research Contract No. 18202 (CRP F23030). Authors acknowledge FCT—Fundação para a Ciência e a Tecnologia I.P. for the national funds in the scope of the project UIDB/04349/2020+UIDP/04349/2020, UIDB/00100/2020 and UIDB/00329/2020. Publisher Copyright: © 2022 by the authors.
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spelling Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Modelcell proliferationchitosangamma radiationgelatinPVAregenerative medicineskin scaffoldsVPPharmaceutical ScienceFunding Information: This research was funded by the International Atomic Energy Agency under Research Contract No. 18202 (CRP F23030). Authors acknowledge FCT—Fundação para a Ciência e a Tecnologia I.P. for the national funds in the scope of the project UIDB/04349/2020+UIDP/04349/2020, UIDB/00100/2020 and UIDB/00329/2020. Publisher Copyright: © 2022 by the authors.Frequently, deep partial and full-thickness skin wounds do not spontaneously regenerate. To restore the normal function of skin, epidermal and dermal components have to be supplied to the wound bed by grafting various substrates. Available options are limited and frequently costly. Herein, authors present a possible approach using 3D skin scaffolds capable of mimicking structure and biological functions of the extracellular matrix, providing, in parallel, a good environment for cell attachment, proliferation and differentiation. Low-molecular weight chitosan-based membranes were prepared by freeze-drying and ionizing radiation techniques to be used as skin scaffolds. Poly (vinyl alcohol), PVA, vinyl pyrrolidone, VP, and gelatin from cold water fish were incorporated. Information regarding membranes’ physical-chemical properties from SEM analysis, swelling and weight loss, together with biological response through in vitro assays (using Human Caucasian Fetal Foreskin Fibroblast) allowed the selection of an optimized batch of membranes that was used as skin scaffold in a dorsal rat model wound. The in vivo implantation assays (in Wistar rats) resulted in very promising results: (i) healing process faster than control; (ii) good vascularization; (iii) viable new tissues morphologically functional.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)RUNCasimiro, Maria HelenaFerreira, Luís M.Santos, Pedro M.P.Leal, João P.Rodrigues, GabrielaIria, InêsAlves, SaraPais, DiogoCasal, Diogo2023-01-13T22:20:12Z2022-122022-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/147552eng1999-4923PURE: 49547609https://doi.org/10.3390/pharmaceutics14122736info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:28:46Zoai:run.unl.pt:10362/147552Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:59.780832Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model
title Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model
spellingShingle Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model
Casimiro, Maria Helena
cell proliferation
chitosan
gamma radiation
gelatin
PVA
regenerative medicine
skin scaffolds
VP
Pharmaceutical Science
title_short Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model
title_full Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model
title_fullStr Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model
title_full_unstemmed Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model
title_sort Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model
author Casimiro, Maria Helena
author_facet Casimiro, Maria Helena
Ferreira, Luís M.
Santos, Pedro M.P.
Leal, João P.
Rodrigues, Gabriela
Iria, Inês
Alves, Sara
Pais, Diogo
Casal, Diogo
author_role author
author2 Ferreira, Luís M.
Santos, Pedro M.P.
Leal, João P.
Rodrigues, Gabriela
Iria, Inês
Alves, Sara
Pais, Diogo
Casal, Diogo
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Centro de Estudos de Doenças Crónicas (CEDOC)
RUN
dc.contributor.author.fl_str_mv Casimiro, Maria Helena
Ferreira, Luís M.
Santos, Pedro M.P.
Leal, João P.
Rodrigues, Gabriela
Iria, Inês
Alves, Sara
Pais, Diogo
Casal, Diogo
dc.subject.por.fl_str_mv cell proliferation
chitosan
gamma radiation
gelatin
PVA
regenerative medicine
skin scaffolds
VP
Pharmaceutical Science
topic cell proliferation
chitosan
gamma radiation
gelatin
PVA
regenerative medicine
skin scaffolds
VP
Pharmaceutical Science
description Funding Information: This research was funded by the International Atomic Energy Agency under Research Contract No. 18202 (CRP F23030). Authors acknowledge FCT—Fundação para a Ciência e a Tecnologia I.P. for the national funds in the scope of the project UIDB/04349/2020+UIDP/04349/2020, UIDB/00100/2020 and UIDB/00329/2020. Publisher Copyright: © 2022 by the authors.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
2022-12-01T00:00:00Z
2023-01-13T22:20:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/147552
url http://hdl.handle.net/10362/147552
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1999-4923
PURE: 49547609
https://doi.org/10.3390/pharmaceutics14122736
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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