An integrated BAC approach to the genomics of cork formation
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/6107 |
Resumo: | Mestrado em Biologia Funcional - Instituto Superior de Agronomia |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
An integrated BAC approach to the genomics of cork formationcorksuberinBAC libraryBESsFISHquantitative real time PCRMestrado em Biologia Funcional - Instituto Superior de AgronomiaCork is produced by Quercus suber, an evergreen oak with major economic and environmental importance in Portugal. Suberin is the main component of cork but little is known about the molecular processes underlying suberin biosynthesis. We screened a Quercus suber bacterial artificial chromosome (BAC) library for the presence of relevant genes for suberin biosynthesis through high-density BAC filters hybridizations and we validated hybridization positive clones by Colony PCR. We analyzed BAC-end sequences (BESs) to characterize the structure and composition of the cork oak genome. And we hybridized BAC DNA with mitotic cork oak chromosomes to obtain a cytogenetic map of Q. suber. Finally, we analyzed expression profiles of candidate genes of cork samples from cork oaks with different cork qualities, using quantitative real-time PCR. We identified several genes involved in suberin biosynthesis pathways. BESs analysis showed high identity with coding regions and suggest the existence of conserved regions within the Fagacea family. We found strong evidence that the genes Cyp 86A1 and GDSL involved in lipid suberin biosynthesis pathway are closely located in the genome. However, we did not succeed in mapping genes of interest in the cork oak genome and we did not find any relation between the genes relative expression and cork qualityThe work was partially supported by a grant from the Fundação para a Ciência e Tecnologia (FCT), project SuberGene (PTDC/AGR-GPL/101785/2008, Genomics of Cork Formation: an integrated approach of cork qualityISAPaiva, Jorge PintoCecílio, Leonor MoraisRepositório da Universidade de LisboaPinto, Maria Joana Amado Teixeira2013-11-06T15:41:23Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/6107TID:201847272engPinto, M.J.A.T. - An integrated BAC approach to the genomics of cork formation. Lisboa: ISA, 2013, 129 p.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:36:58Zoai:www.repository.utl.pt:10400.5/6107Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:53:32.583820Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
An integrated BAC approach to the genomics of cork formation |
title |
An integrated BAC approach to the genomics of cork formation |
spellingShingle |
An integrated BAC approach to the genomics of cork formation Pinto, Maria Joana Amado Teixeira cork suberin BAC library BESs FISH quantitative real time PCR |
title_short |
An integrated BAC approach to the genomics of cork formation |
title_full |
An integrated BAC approach to the genomics of cork formation |
title_fullStr |
An integrated BAC approach to the genomics of cork formation |
title_full_unstemmed |
An integrated BAC approach to the genomics of cork formation |
title_sort |
An integrated BAC approach to the genomics of cork formation |
author |
Pinto, Maria Joana Amado Teixeira |
author_facet |
Pinto, Maria Joana Amado Teixeira |
author_role |
author |
dc.contributor.none.fl_str_mv |
Paiva, Jorge Pinto Cecílio, Leonor Morais Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Pinto, Maria Joana Amado Teixeira |
dc.subject.por.fl_str_mv |
cork suberin BAC library BESs FISH quantitative real time PCR |
topic |
cork suberin BAC library BESs FISH quantitative real time PCR |
description |
Mestrado em Biologia Funcional - Instituto Superior de Agronomia |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-11-06T15:41:23Z 2013 2013-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/6107 TID:201847272 |
url |
http://hdl.handle.net/10400.5/6107 |
identifier_str_mv |
TID:201847272 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pinto, M.J.A.T. - An integrated BAC approach to the genomics of cork formation. Lisboa: ISA, 2013, 129 p. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
ISA |
publisher.none.fl_str_mv |
ISA |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799131011251961856 |