Scavenging capacity of strawberry tree (Arbutus unedo L.) leaves on free radicals
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/3106 |
Resumo: | Despite strawberry tree (Arbutus unedo L.) leaves had a long use in traditional medicine due to its antiseptic, diuretic, astringent and depurative properties, the potential of their antioxidant activity are still lacking. Our study goals to assess the antioxidant and free radical scavenging potential of water, ethanol, methanol and diethyl ether extracts of A. unedo leaves. Total phenols content was achieved spectrophotometrically using Folin–Ciocalteau reagent with gallic acid as standard. Antioxidant activity was evaluated using three different methods: reducing power of iron (III)/ferricyanide complex assay, scavenging effect on DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and scavenging effect on superoxide radicals by using the PMS–NADH–nitroblue tetrazolium system. Ethanol extracts of A. unedo leaves were the highest in reducing power (IC50 232.7 lg/mL) and DPPH scavenging effect (IC50 63.2 lg/mL) followed by water extracts (with IC50 of 287.7 and 73.7 lg/mL, respectively); whereas diethyl ether extracts were the lowest. In the scavenging on superoxide radical assay, methanol extracts obtained the best results (IC50 6.9 lg/mL). For all the methods tested the antioxidant activity was concentration dependent. In accordance with antioxidant activity, highest total phenols content were found in ethanol, followed by water, methanol and diethyl ether extract. The results indicated that A. unedo leaves are a potential source of natural antioxidants. |
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Scavenging capacity of strawberry tree (Arbutus unedo L.) leaves on free radicalsArbutus unedo L. leavesExtraction methodTotal phenolsAntioxidant activitySuperoxide scavengingDespite strawberry tree (Arbutus unedo L.) leaves had a long use in traditional medicine due to its antiseptic, diuretic, astringent and depurative properties, the potential of their antioxidant activity are still lacking. Our study goals to assess the antioxidant and free radical scavenging potential of water, ethanol, methanol and diethyl ether extracts of A. unedo leaves. Total phenols content was achieved spectrophotometrically using Folin–Ciocalteau reagent with gallic acid as standard. Antioxidant activity was evaluated using three different methods: reducing power of iron (III)/ferricyanide complex assay, scavenging effect on DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and scavenging effect on superoxide radicals by using the PMS–NADH–nitroblue tetrazolium system. Ethanol extracts of A. unedo leaves were the highest in reducing power (IC50 232.7 lg/mL) and DPPH scavenging effect (IC50 63.2 lg/mL) followed by water extracts (with IC50 of 287.7 and 73.7 lg/mL, respectively); whereas diethyl ether extracts were the lowest. In the scavenging on superoxide radical assay, methanol extracts obtained the best results (IC50 6.9 lg/mL). For all the methods tested the antioxidant activity was concentration dependent. In accordance with antioxidant activity, highest total phenols content were found in ethanol, followed by water, methanol and diethyl ether extract. The results indicated that A. unedo leaves are a potential source of natural antioxidants.ElsevierBiblioteca Digital do IPBOliveira, IvoCoelho, ValentimBaltasar, RaquelPereira, J.A.Baptista, Paula2011-01-13T16:06:14Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/3106engOliveira, I.; Coelho, V.; Baltasar, R.; Pereira, J.A.; Baptista, P. (2009). Scavenging capacity of strawberry tree (Arbutus unedo L.) leaves on free radicals. Food and Chemical Toxicology. ISSN 0278-6915. 47:7, p. 1507-15110278-691510.1016/j.fct.2009.03.042info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:07:59Zoai:bibliotecadigital.ipb.pt:10198/3106Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:55:59.659368Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Scavenging capacity of strawberry tree (Arbutus unedo L.) leaves on free radicals |
title |
Scavenging capacity of strawberry tree (Arbutus unedo L.) leaves on free radicals |
spellingShingle |
Scavenging capacity of strawberry tree (Arbutus unedo L.) leaves on free radicals Oliveira, Ivo Arbutus unedo L. leaves Extraction method Total phenols Antioxidant activity Superoxide scavenging |
title_short |
Scavenging capacity of strawberry tree (Arbutus unedo L.) leaves on free radicals |
title_full |
Scavenging capacity of strawberry tree (Arbutus unedo L.) leaves on free radicals |
title_fullStr |
Scavenging capacity of strawberry tree (Arbutus unedo L.) leaves on free radicals |
title_full_unstemmed |
Scavenging capacity of strawberry tree (Arbutus unedo L.) leaves on free radicals |
title_sort |
Scavenging capacity of strawberry tree (Arbutus unedo L.) leaves on free radicals |
author |
Oliveira, Ivo |
author_facet |
Oliveira, Ivo Coelho, Valentim Baltasar, Raquel Pereira, J.A. Baptista, Paula |
author_role |
author |
author2 |
Coelho, Valentim Baltasar, Raquel Pereira, J.A. Baptista, Paula |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Oliveira, Ivo Coelho, Valentim Baltasar, Raquel Pereira, J.A. Baptista, Paula |
dc.subject.por.fl_str_mv |
Arbutus unedo L. leaves Extraction method Total phenols Antioxidant activity Superoxide scavenging |
topic |
Arbutus unedo L. leaves Extraction method Total phenols Antioxidant activity Superoxide scavenging |
description |
Despite strawberry tree (Arbutus unedo L.) leaves had a long use in traditional medicine due to its antiseptic, diuretic, astringent and depurative properties, the potential of their antioxidant activity are still lacking. Our study goals to assess the antioxidant and free radical scavenging potential of water, ethanol, methanol and diethyl ether extracts of A. unedo leaves. Total phenols content was achieved spectrophotometrically using Folin–Ciocalteau reagent with gallic acid as standard. Antioxidant activity was evaluated using three different methods: reducing power of iron (III)/ferricyanide complex assay, scavenging effect on DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and scavenging effect on superoxide radicals by using the PMS–NADH–nitroblue tetrazolium system. Ethanol extracts of A. unedo leaves were the highest in reducing power (IC50 232.7 lg/mL) and DPPH scavenging effect (IC50 63.2 lg/mL) followed by water extracts (with IC50 of 287.7 and 73.7 lg/mL, respectively); whereas diethyl ether extracts were the lowest. In the scavenging on superoxide radical assay, methanol extracts obtained the best results (IC50 6.9 lg/mL). For all the methods tested the antioxidant activity was concentration dependent. In accordance with antioxidant activity, highest total phenols content were found in ethanol, followed by water, methanol and diethyl ether extract. The results indicated that A. unedo leaves are a potential source of natural antioxidants. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2009-01-01T00:00:00Z 2011-01-13T16:06:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/3106 |
url |
http://hdl.handle.net/10198/3106 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oliveira, I.; Coelho, V.; Baltasar, R.; Pereira, J.A.; Baptista, P. (2009). Scavenging capacity of strawberry tree (Arbutus unedo L.) leaves on free radicals. Food and Chemical Toxicology. ISSN 0278-6915. 47:7, p. 1507-1511 0278-6915 10.1016/j.fct.2009.03.042 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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