Redox-Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy

Detalhes bibliográficos
Autor(a) principal: Valentim-Coelho, Cristina
Data de Publicação: 2020
Outros Autores: Vaz, Fátima, Antunes, Marília, Neves, Sofia, Martins, Inês L, Osório, Hugo, Feliciano, Amélia, Pinto, Paula, Bárbara, Cristina, Penque, Deborah
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/115883
Resumo: Funding: Project partially supported by Harvard Medical School-Portugal Program (HMSPICJ/0022/2011), ToxOmics—Centre for Toxicogenomics and Human Health (FCT-UID/BIM/00009/2013), Fundação para a Ciência e a Tecnologia (FCT)/Poly-Annual Funding Program and FEDER/Saúde XXI Program, Portugal, Antioxidants 2020, 9, 1184 26 of 29 RNEM- National Mass Spectrometry Networking—FCT Strategic Infrastructure and PhD fellowship, FCT-SFRH/BD/133511/2017., Portugal
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spelling Redox-Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure TherapyFunding: Project partially supported by Harvard Medical School-Portugal Program (HMSPICJ/0022/2011), ToxOmics—Centre for Toxicogenomics and Human Health (FCT-UID/BIM/00009/2013), Fundação para a Ciência e a Tecnologia (FCT)/Poly-Annual Funding Program and FEDER/Saúde XXI Program, Portugal, Antioxidants 2020, 9, 1184 26 of 29 RNEM- National Mass Spectrometry Networking—FCT Strategic Infrastructure and PhD fellowship, FCT-SFRH/BD/133511/2017., PortugalIn this study, we examined the effect of six months of positive airway pressure (PAP) therapy on Obstructive Sleep Apnea (OSA) red blood cell (RBC) proteome by two dimensional difference gel electrophoresis (2D-DIGE) - based proteomics followed by Western blotting (WB) validation. The discovered dysregulated proteins/proteoforms are associated with cell death, H2O2 catabolic/metabolic process, stress response, and protein oligomerization. Validation by nonreducing WB was performed for peroxiredoxin-2 (PRDX2) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by using antibodies against the sulfinylated/sulfonylated cysteine of these proteins to better evaluate their redox-oligomeric states under OSA and/or in response to PAP therapy. The results indicated that the redox-oligomeric state of GAPDH and PRDX2 involving overoxidation by sulfinic/sulfonic acids were differentially modulated in OSA RBC, which might be compromising RBC homeostasis. PAP therapy by restoring this modulation induced a higher oligomerization of overoxidized GAPDH and PRDX2 in some patients that could be associated with eryptosis and the chaperone "gain" of function, respectively. This varied response following PAP may result from the complex interplay between OSA and OSA metabolic comorbidity. Hence, information on the redox status of PRDX2 and GAPDH in RBC will help to better recognize OSA subtypes and predict the therapeutic response in these patients. GAPDH monomer combined with body mass index (BMI) and PRDX2 S-S dimer combined with homeostatic model assessment for insulin resistance (HOMA-IR) showed to be very promising biomarkers to predict OSA and OSA severity, respectively.Centre for Toxicogenomics and Human Health (ToxOmics)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNValentim-Coelho, CristinaVaz, FátimaAntunes, MaríliaNeves, SofiaMartins, Inês LOsório, HugoFeliciano, AméliaPinto, PaulaBárbara, CristinaPenque, Deborah2021-04-20T22:51:04Z2020-11-262020-11-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/115883eng2076-3921PURE: 29186171https://doi.org/10.3390/antiox9121184info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:58:26Zoai:run.unl.pt:10362/115883Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:42:52.153608Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Redox-Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy
title Redox-Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy
spellingShingle Redox-Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy
Valentim-Coelho, Cristina
title_short Redox-Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy
title_full Redox-Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy
title_fullStr Redox-Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy
title_full_unstemmed Redox-Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy
title_sort Redox-Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy
author Valentim-Coelho, Cristina
author_facet Valentim-Coelho, Cristina
Vaz, Fátima
Antunes, Marília
Neves, Sofia
Martins, Inês L
Osório, Hugo
Feliciano, Amélia
Pinto, Paula
Bárbara, Cristina
Penque, Deborah
author_role author
author2 Vaz, Fátima
Antunes, Marília
Neves, Sofia
Martins, Inês L
Osório, Hugo
Feliciano, Amélia
Pinto, Paula
Bárbara, Cristina
Penque, Deborah
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centre for Toxicogenomics and Human Health (ToxOmics)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Valentim-Coelho, Cristina
Vaz, Fátima
Antunes, Marília
Neves, Sofia
Martins, Inês L
Osório, Hugo
Feliciano, Amélia
Pinto, Paula
Bárbara, Cristina
Penque, Deborah
description Funding: Project partially supported by Harvard Medical School-Portugal Program (HMSPICJ/0022/2011), ToxOmics—Centre for Toxicogenomics and Human Health (FCT-UID/BIM/00009/2013), Fundação para a Ciência e a Tecnologia (FCT)/Poly-Annual Funding Program and FEDER/Saúde XXI Program, Portugal, Antioxidants 2020, 9, 1184 26 of 29 RNEM- National Mass Spectrometry Networking—FCT Strategic Infrastructure and PhD fellowship, FCT-SFRH/BD/133511/2017., Portugal
publishDate 2020
dc.date.none.fl_str_mv 2020-11-26
2020-11-26T00:00:00Z
2021-04-20T22:51:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/115883
url http://hdl.handle.net/10362/115883
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2076-3921
PURE: 29186171
https://doi.org/10.3390/antiox9121184
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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