Alginate particles for encapsulation of phenolic extract from Spirulina sp. LEB-18: physicochemical characterization and assessment of in vitro gastrointestinal behavior
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/80669 |
Resumo: | Encapsulation can be used as a strategy to protect and control the release of bioactive extracts. In this work, an extract from Spirulina sp. LEB-18, rich in phenolic compounds, was encapsulated in biopolymeric particles (i.e., composed of alginate) and characterized concerning their thermal behavior using differential scanning calorimetry (DSC), size, morphology, swelling index (S), and encapsulation efficiency (EE%); the release profile of the phenolic compounds at different pHs and the particle behavior under in vitro gastrointestinal digestion were also evaluated. It was shown that it is possible to encapsulate the phenolic extract from Spirulina sp. LEB-18 in alginate particles with high encapsulation efficiency (88.97%). It was also observed that the particles are amorphous and that the encapsulated phenolic compounds were released at a pH 7.2 but not at pH 1.5, which means that the alginate particles are able to protect the phenolic compounds from the harsh stomach conditions but lose their integrity under intestinal pH conditions. Regarding bioaccessibility, it was observed that the encapsulated phenolic compounds showed higher bioaccessibility compared to phenolic compounds in free form. This work increases the knowledge about the behavior of alginate particles encapsulating phenolic compounds during in vitro gastrointestinal digestion. It also provides essential information for designing biopolymeric particle formulations encapsulating phenolic compounds for application in pharmaceutical and food products. |
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Alginate particles for encapsulation of phenolic extract from Spirulina sp. LEB-18: physicochemical characterization and assessment of in vitro gastrointestinal behaviorantioxidantbiopolymerscontrolled releasedigestionScience & TechnologyEncapsulation can be used as a strategy to protect and control the release of bioactive extracts. In this work, an extract from Spirulina sp. LEB-18, rich in phenolic compounds, was encapsulated in biopolymeric particles (i.e., composed of alginate) and characterized concerning their thermal behavior using differential scanning calorimetry (DSC), size, morphology, swelling index (S), and encapsulation efficiency (EE%); the release profile of the phenolic compounds at different pHs and the particle behavior under in vitro gastrointestinal digestion were also evaluated. It was shown that it is possible to encapsulate the phenolic extract from Spirulina sp. LEB-18 in alginate particles with high encapsulation efficiency (88.97%). It was also observed that the particles are amorphous and that the encapsulated phenolic compounds were released at a pH 7.2 but not at pH 1.5, which means that the alginate particles are able to protect the phenolic compounds from the harsh stomach conditions but lose their integrity under intestinal pH conditions. Regarding bioaccessibility, it was observed that the encapsulated phenolic compounds showed higher bioaccessibility compared to phenolic compounds in free form. This work increases the knowledge about the behavior of alginate particles encapsulating phenolic compounds during in vitro gastrointestinal digestion. It also provides essential information for designing biopolymeric particle formulations encapsulating phenolic compounds for application in pharmaceutical and food products.The study was financially supported by the following programs and institutions: Science Without Borders—CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), CSF (Ciência sem fronteiras), CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), Brazil, the University of Minho, Braga, Portugal, and Foundation for Science and Technology (FCT, POPH-QREN and FSE Portugal). This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit, and by LABBELS–Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoMachado, Adriana R.Silva, PedroVicente, A. A.Souza-Soares, Leonor A.Pinheiro, A. C.Cerqueira, Miguel Ângelo Parente Ribeiro2022-11-062022-11-06T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/80669engMachado, A.R.; Silva, P.M.P.; Vicente, A.A.; Souza-Soares, L.A.; Pinheiro, A.C.; Cerqueira, M.A. Alginate Particles for Encapsulation of Phenolic Extract from Spirulina sp. LEB-18: Physicochemical Characterization and Assessment of In Vitro Gastrointestinal Behavior. Polymers 2022, 14, 4759. https://doi.org/10.3390/polym142147592073-436010.3390/polym14214759https://www.mdpi.com/2073-4360/14/21/4759info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-12T01:17:33ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Alginate particles for encapsulation of phenolic extract from Spirulina sp. LEB-18: physicochemical characterization and assessment of in vitro gastrointestinal behavior |
title |
Alginate particles for encapsulation of phenolic extract from Spirulina sp. LEB-18: physicochemical characterization and assessment of in vitro gastrointestinal behavior |
spellingShingle |
Alginate particles for encapsulation of phenolic extract from Spirulina sp. LEB-18: physicochemical characterization and assessment of in vitro gastrointestinal behavior Machado, Adriana R. antioxidant biopolymers controlled release digestion Science & Technology |
title_short |
Alginate particles for encapsulation of phenolic extract from Spirulina sp. LEB-18: physicochemical characterization and assessment of in vitro gastrointestinal behavior |
title_full |
Alginate particles for encapsulation of phenolic extract from Spirulina sp. LEB-18: physicochemical characterization and assessment of in vitro gastrointestinal behavior |
title_fullStr |
Alginate particles for encapsulation of phenolic extract from Spirulina sp. LEB-18: physicochemical characterization and assessment of in vitro gastrointestinal behavior |
title_full_unstemmed |
Alginate particles for encapsulation of phenolic extract from Spirulina sp. LEB-18: physicochemical characterization and assessment of in vitro gastrointestinal behavior |
title_sort |
Alginate particles for encapsulation of phenolic extract from Spirulina sp. LEB-18: physicochemical characterization and assessment of in vitro gastrointestinal behavior |
author |
Machado, Adriana R. |
author_facet |
Machado, Adriana R. Silva, Pedro Vicente, A. A. Souza-Soares, Leonor A. Pinheiro, A. C. Cerqueira, Miguel Ângelo Parente Ribeiro |
author_role |
author |
author2 |
Silva, Pedro Vicente, A. A. Souza-Soares, Leonor A. Pinheiro, A. C. Cerqueira, Miguel Ângelo Parente Ribeiro |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Machado, Adriana R. Silva, Pedro Vicente, A. A. Souza-Soares, Leonor A. Pinheiro, A. C. Cerqueira, Miguel Ângelo Parente Ribeiro |
dc.subject.por.fl_str_mv |
antioxidant biopolymers controlled release digestion Science & Technology |
topic |
antioxidant biopolymers controlled release digestion Science & Technology |
description |
Encapsulation can be used as a strategy to protect and control the release of bioactive extracts. In this work, an extract from Spirulina sp. LEB-18, rich in phenolic compounds, was encapsulated in biopolymeric particles (i.e., composed of alginate) and characterized concerning their thermal behavior using differential scanning calorimetry (DSC), size, morphology, swelling index (S), and encapsulation efficiency (EE%); the release profile of the phenolic compounds at different pHs and the particle behavior under in vitro gastrointestinal digestion were also evaluated. It was shown that it is possible to encapsulate the phenolic extract from Spirulina sp. LEB-18 in alginate particles with high encapsulation efficiency (88.97%). It was also observed that the particles are amorphous and that the encapsulated phenolic compounds were released at a pH 7.2 but not at pH 1.5, which means that the alginate particles are able to protect the phenolic compounds from the harsh stomach conditions but lose their integrity under intestinal pH conditions. Regarding bioaccessibility, it was observed that the encapsulated phenolic compounds showed higher bioaccessibility compared to phenolic compounds in free form. This work increases the knowledge about the behavior of alginate particles encapsulating phenolic compounds during in vitro gastrointestinal digestion. It also provides essential information for designing biopolymeric particle formulations encapsulating phenolic compounds for application in pharmaceutical and food products. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-06 2022-11-06T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/80669 |
url |
https://hdl.handle.net/1822/80669 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Machado, A.R.; Silva, P.M.P.; Vicente, A.A.; Souza-Soares, L.A.; Pinheiro, A.C.; Cerqueira, M.A. Alginate Particles for Encapsulation of Phenolic Extract from Spirulina sp. LEB-18: Physicochemical Characterization and Assessment of In Vitro Gastrointestinal Behavior. Polymers 2022, 14, 4759. https://doi.org/10.3390/polym14214759 2073-4360 10.3390/polym14214759 https://www.mdpi.com/2073-4360/14/21/4759 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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