Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/98795 |
Resumo: | The authors would also like to acknowledge the Fundacao para a Ciencia e a Tecnologia (Portugal) for funding through SFRH/BPD/101642/2014. co-financed by ERDF under PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). |
id |
RCAP_6e337923dbd3af52a11986acf41c15cd |
---|---|
oai_identifier_str |
oai:run.unl.pt:10362/98795 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditionsFermented cheese whey (FCW)Phototrophic mixed cultures (PMCs)Polyhydroxyalkanoates (PHA)Purple phototrophic bacteria (PPB)Volatile fatty acids (VFAs)Structural BiologyBiochemistryMolecular BiologyThe authors would also like to acknowledge the Fundacao para a Ciencia e a Tecnologia (Portugal) for funding through SFRH/BPD/101642/2014. co-financed by ERDF under PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728).Polyhydroxyalkanoates (PHAs) production with phototrophic mixed cultures (PMCs) has been recently proposed. These cultures can be selected under the permanent presence of carbon and the PHA production can be enhanced in subsequent accumulation steps. To optimize the PHA production in accumulator reactors, this work evaluated the impact of 1) initial acetate concentration, 2) light intensity, 3) removal of residual nitrogen on the culture performance. Results indicate that low acetate concentration (<30 CmM) and specific light intensities around 20 W/gX are optimal operating conditions that lead to high polyhydroxybutyrate (PHB) storage yields (0.83 ± 0.07 Cmol-PHB/Cmol-Acet) and specific PHB production rates of 2.21 ± 0.07 Cmol-PHB/Cmol X d. This rate is three times higher than previously registered in non-optimized accumulation tests and enabled a PHA content increase from 15 to 30% in <4 h. Also, it was shown for the first time, the capability of a PMC to use a real waste, fermented cheese whey, to produce PHA with a hydroxyvalerate (HV) content of 12%. These results confirm that fermented wastes can be used as substrates for PHA production with PMCs and that the energy levels in sunlight that lead to specific light intensities from 10 to 20 W/gX are sufficient to drive phototrophic PHA production processes.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNFradinho, J. C.Oehmen, A.Reis, M. A. M.2020-06-03T10:25:41Z2019-04-012019-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://hdl.handle.net/10362/98795eng0141-8130PURE: 11474735https://doi.org/10.1016/j.ijbiomac.2018.12.270info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:45:59Zoai:run.unl.pt:10362/98795Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:39:03.273362Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions |
title |
Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions |
spellingShingle |
Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions Fradinho, J. C. Fermented cheese whey (FCW) Phototrophic mixed cultures (PMCs) Polyhydroxyalkanoates (PHA) Purple phototrophic bacteria (PPB) Volatile fatty acids (VFAs) Structural Biology Biochemistry Molecular Biology |
title_short |
Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions |
title_full |
Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions |
title_fullStr |
Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions |
title_full_unstemmed |
Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions |
title_sort |
Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions |
author |
Fradinho, J. C. |
author_facet |
Fradinho, J. C. Oehmen, A. Reis, M. A. M. |
author_role |
author |
author2 |
Oehmen, A. Reis, M. A. M. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
UCIBIO - Applied Molecular Biosciences Unit DQ - Departamento de Química RUN |
dc.contributor.author.fl_str_mv |
Fradinho, J. C. Oehmen, A. Reis, M. A. M. |
dc.subject.por.fl_str_mv |
Fermented cheese whey (FCW) Phototrophic mixed cultures (PMCs) Polyhydroxyalkanoates (PHA) Purple phototrophic bacteria (PPB) Volatile fatty acids (VFAs) Structural Biology Biochemistry Molecular Biology |
topic |
Fermented cheese whey (FCW) Phototrophic mixed cultures (PMCs) Polyhydroxyalkanoates (PHA) Purple phototrophic bacteria (PPB) Volatile fatty acids (VFAs) Structural Biology Biochemistry Molecular Biology |
description |
The authors would also like to acknowledge the Fundacao para a Ciencia e a Tecnologia (Portugal) for funding through SFRH/BPD/101642/2014. co-financed by ERDF under PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-04-01 2019-04-01T00:00:00Z 2020-06-03T10:25:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/98795 |
url |
http://hdl.handle.net/10362/98795 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0141-8130 PURE: 11474735 https://doi.org/10.1016/j.ijbiomac.2018.12.270 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799138006846668800 |