Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients

Detalhes bibliográficos
Autor(a) principal: Quero, Javier
Data de Publicação: 2022
Outros Autores: Ballesteros, Lina F., Santos, Pedro Miguel Ferreira, Velderrain-Rodriguez, Gustavo R., Rocha, Cristina M. R., Pereira, Ricardo Nuno Correia, Teixeira, J. A., Martin-Belloso, Olga, Osada, Jesús, Rodríguez-Yoldi, María Jesús
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/77257
Resumo: Olive pomace (OP) is the main residue that results from olive oil production. OP is rich in bioactive compounds, including polyphenols, so its use in the treatments of diseases related to oxidative stress, such as cancer, could be considered. The present work aimed to study the biological properties of different OP extracts, obtained by ohmic heating-assisted extraction and conventional heating, using water and 50% ethanol, in the treatment and prevention of colorectal cancer through Caco-2 cell models. Additionally, an in-silico analysis was performed to identify the phenolic intestinal absorption and Caco-2 permeability. The extracts were chemically characterized, and it was found that the Ohmic-hydroethanolic (OH-EtOH) extract had the highest antiproliferative effect, probably due to its higher content of phenolic compounds. The OH-EtOH induced potential modifications in the mitochondrial membrane and led to apoptosis by cell cycle arrest in the G1/S phases with activation of p53 and caspase 3 proteins. In addition, this extract protected the intestine against oxidative stress (ROS) caused by H2O2. Therefore, the bioactive compounds present in OP and recovered by applying a green technology such as ohmic-heating, show promising potential to be used in food, nutraceutical, and biomedical applications, reducing this waste and facilitating the circular economy.
id RCAP_70cb2474010ca7bbdfd087192f132a4a
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/77257
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredientsolive pomace wasteohmic heatingapoptosisCaco-2 cellsROSScience & TechnologyOlive pomace (OP) is the main residue that results from olive oil production. OP is rich in bioactive compounds, including polyphenols, so its use in the treatments of diseases related to oxidative stress, such as cancer, could be considered. The present work aimed to study the biological properties of different OP extracts, obtained by ohmic heating-assisted extraction and conventional heating, using water and 50% ethanol, in the treatment and prevention of colorectal cancer through Caco-2 cell models. Additionally, an in-silico analysis was performed to identify the phenolic intestinal absorption and Caco-2 permeability. The extracts were chemically characterized, and it was found that the Ohmic-hydroethanolic (OH-EtOH) extract had the highest antiproliferative effect, probably due to its higher content of phenolic compounds. The OH-EtOH induced potential modifications in the mitochondrial membrane and led to apoptosis by cell cycle arrest in the G1/S phases with activation of p53 and caspase 3 proteins. In addition, this extract protected the intestine against oxidative stress (ROS) caused by H2O2. Therefore, the bioactive compounds present in OP and recovered by applying a green technology such as ohmic-heating, show promising potential to be used in food, nutraceutical, and biomedical applications, reducing this waste and facilitating the circular economy.This work was supported by the Grants from MCIN, CIBEROBN (CB06/03/1012), Red Multimetdrugs (RED2018-102471-T), and the Government of Aragón (B16-20R), the Interreg Sudoe Program (REDVALUE, SOE1/P1/E0123) through the European Regional Development Fund (FEDER) and the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit.info:eu-repo/semantics/publishedVersionMDPI AGUniversidade do MinhoQuero, JavierBallesteros, Lina F.Santos, Pedro Miguel FerreiraVelderrain-Rodriguez, Gustavo R.Rocha, Cristina M. R.Pereira, Ricardo Nuno CorreiaTeixeira, J. A.Martin-Belloso, OlgaOsada, JesúsRodríguez-Yoldi, María Jesús20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/77257engQuero, Javier; Ballesteros, Lina F.; Ferreira-Santos, P.; Velderrain-Rodriguez, Gustavo R.; Rocha, Cristina M. R.; Pereira, Ricardo N.; Teixeira, José A.; Martin-Belloso, Olga; Osada, Jesús; Rodríguez-Yoldi, María Jesús, Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients. Antioxidants, 11(5), 828, 20222076-392110.3390/antiox11050828https://www.mdpi.com/journal/antioxidantsinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:48:19Zoai:repositorium.sdum.uminho.pt:1822/77257Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:46:33.038210Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients
title Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients
spellingShingle Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients
Quero, Javier
olive pomace waste
ohmic heating
apoptosis
Caco-2 cells
ROS
Science & Technology
title_short Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients
title_full Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients
title_fullStr Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients
title_full_unstemmed Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients
title_sort Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients
author Quero, Javier
author_facet Quero, Javier
Ballesteros, Lina F.
Santos, Pedro Miguel Ferreira
Velderrain-Rodriguez, Gustavo R.
Rocha, Cristina M. R.
Pereira, Ricardo Nuno Correia
Teixeira, J. A.
Martin-Belloso, Olga
Osada, Jesús
Rodríguez-Yoldi, María Jesús
author_role author
author2 Ballesteros, Lina F.
Santos, Pedro Miguel Ferreira
Velderrain-Rodriguez, Gustavo R.
Rocha, Cristina M. R.
Pereira, Ricardo Nuno Correia
Teixeira, J. A.
Martin-Belloso, Olga
Osada, Jesús
Rodríguez-Yoldi, María Jesús
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Quero, Javier
Ballesteros, Lina F.
Santos, Pedro Miguel Ferreira
Velderrain-Rodriguez, Gustavo R.
Rocha, Cristina M. R.
Pereira, Ricardo Nuno Correia
Teixeira, J. A.
Martin-Belloso, Olga
Osada, Jesús
Rodríguez-Yoldi, María Jesús
dc.subject.por.fl_str_mv olive pomace waste
ohmic heating
apoptosis
Caco-2 cells
ROS
Science & Technology
topic olive pomace waste
ohmic heating
apoptosis
Caco-2 cells
ROS
Science & Technology
description Olive pomace (OP) is the main residue that results from olive oil production. OP is rich in bioactive compounds, including polyphenols, so its use in the treatments of diseases related to oxidative stress, such as cancer, could be considered. The present work aimed to study the biological properties of different OP extracts, obtained by ohmic heating-assisted extraction and conventional heating, using water and 50% ethanol, in the treatment and prevention of colorectal cancer through Caco-2 cell models. Additionally, an in-silico analysis was performed to identify the phenolic intestinal absorption and Caco-2 permeability. The extracts were chemically characterized, and it was found that the Ohmic-hydroethanolic (OH-EtOH) extract had the highest antiproliferative effect, probably due to its higher content of phenolic compounds. The OH-EtOH induced potential modifications in the mitochondrial membrane and led to apoptosis by cell cycle arrest in the G1/S phases with activation of p53 and caspase 3 proteins. In addition, this extract protected the intestine against oxidative stress (ROS) caused by H2O2. Therefore, the bioactive compounds present in OP and recovered by applying a green technology such as ohmic-heating, show promising potential to be used in food, nutraceutical, and biomedical applications, reducing this waste and facilitating the circular economy.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/77257
url https://hdl.handle.net/1822/77257
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Quero, Javier; Ballesteros, Lina F.; Ferreira-Santos, P.; Velderrain-Rodriguez, Gustavo R.; Rocha, Cristina M. R.; Pereira, Ricardo N.; Teixeira, José A.; Martin-Belloso, Olga; Osada, Jesús; Rodríguez-Yoldi, María Jesús, Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients. Antioxidants, 11(5), 828, 2022
2076-3921
10.3390/antiox11050828
https://www.mdpi.com/journal/antioxidants
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799133034779246592