Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/77257 |
Resumo: | Olive pomace (OP) is the main residue that results from olive oil production. OP is rich in bioactive compounds, including polyphenols, so its use in the treatments of diseases related to oxidative stress, such as cancer, could be considered. The present work aimed to study the biological properties of different OP extracts, obtained by ohmic heating-assisted extraction and conventional heating, using water and 50% ethanol, in the treatment and prevention of colorectal cancer through Caco-2 cell models. Additionally, an in-silico analysis was performed to identify the phenolic intestinal absorption and Caco-2 permeability. The extracts were chemically characterized, and it was found that the Ohmic-hydroethanolic (OH-EtOH) extract had the highest antiproliferative effect, probably due to its higher content of phenolic compounds. The OH-EtOH induced potential modifications in the mitochondrial membrane and led to apoptosis by cell cycle arrest in the G1/S phases with activation of p53 and caspase 3 proteins. In addition, this extract protected the intestine against oxidative stress (ROS) caused by H2O2. Therefore, the bioactive compounds present in OP and recovered by applying a green technology such as ohmic-heating, show promising potential to be used in food, nutraceutical, and biomedical applications, reducing this waste and facilitating the circular economy. |
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Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredientsolive pomace wasteohmic heatingapoptosisCaco-2 cellsROSScience & TechnologyOlive pomace (OP) is the main residue that results from olive oil production. OP is rich in bioactive compounds, including polyphenols, so its use in the treatments of diseases related to oxidative stress, such as cancer, could be considered. The present work aimed to study the biological properties of different OP extracts, obtained by ohmic heating-assisted extraction and conventional heating, using water and 50% ethanol, in the treatment and prevention of colorectal cancer through Caco-2 cell models. Additionally, an in-silico analysis was performed to identify the phenolic intestinal absorption and Caco-2 permeability. The extracts were chemically characterized, and it was found that the Ohmic-hydroethanolic (OH-EtOH) extract had the highest antiproliferative effect, probably due to its higher content of phenolic compounds. The OH-EtOH induced potential modifications in the mitochondrial membrane and led to apoptosis by cell cycle arrest in the G1/S phases with activation of p53 and caspase 3 proteins. In addition, this extract protected the intestine against oxidative stress (ROS) caused by H2O2. Therefore, the bioactive compounds present in OP and recovered by applying a green technology such as ohmic-heating, show promising potential to be used in food, nutraceutical, and biomedical applications, reducing this waste and facilitating the circular economy.This work was supported by the Grants from MCIN, CIBEROBN (CB06/03/1012), Red Multimetdrugs (RED2018-102471-T), and the Government of Aragón (B16-20R), the Interreg Sudoe Program (REDVALUE, SOE1/P1/E0123) through the European Regional Development Fund (FEDER) and the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit.info:eu-repo/semantics/publishedVersionMDPI AGUniversidade do MinhoQuero, JavierBallesteros, Lina F.Santos, Pedro Miguel FerreiraVelderrain-Rodriguez, Gustavo R.Rocha, Cristina M. R.Pereira, Ricardo Nuno CorreiaTeixeira, J. A.Martin-Belloso, OlgaOsada, JesúsRodríguez-Yoldi, María Jesús20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/77257engQuero, Javier; Ballesteros, Lina F.; Ferreira-Santos, P.; Velderrain-Rodriguez, Gustavo R.; Rocha, Cristina M. R.; Pereira, Ricardo N.; Teixeira, José A.; Martin-Belloso, Olga; Osada, Jesús; Rodríguez-Yoldi, María Jesús, Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients. Antioxidants, 11(5), 828, 20222076-392110.3390/antiox11050828https://www.mdpi.com/journal/antioxidantsinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:48:19Zoai:repositorium.sdum.uminho.pt:1822/77257Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:46:33.038210Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients |
title |
Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients |
spellingShingle |
Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients Quero, Javier olive pomace waste ohmic heating apoptosis Caco-2 cells ROS Science & Technology |
title_short |
Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients |
title_full |
Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients |
title_fullStr |
Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients |
title_full_unstemmed |
Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients |
title_sort |
Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients |
author |
Quero, Javier |
author_facet |
Quero, Javier Ballesteros, Lina F. Santos, Pedro Miguel Ferreira Velderrain-Rodriguez, Gustavo R. Rocha, Cristina M. R. Pereira, Ricardo Nuno Correia Teixeira, J. A. Martin-Belloso, Olga Osada, Jesús Rodríguez-Yoldi, María Jesús |
author_role |
author |
author2 |
Ballesteros, Lina F. Santos, Pedro Miguel Ferreira Velderrain-Rodriguez, Gustavo R. Rocha, Cristina M. R. Pereira, Ricardo Nuno Correia Teixeira, J. A. Martin-Belloso, Olga Osada, Jesús Rodríguez-Yoldi, María Jesús |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Quero, Javier Ballesteros, Lina F. Santos, Pedro Miguel Ferreira Velderrain-Rodriguez, Gustavo R. Rocha, Cristina M. R. Pereira, Ricardo Nuno Correia Teixeira, J. A. Martin-Belloso, Olga Osada, Jesús Rodríguez-Yoldi, María Jesús |
dc.subject.por.fl_str_mv |
olive pomace waste ohmic heating apoptosis Caco-2 cells ROS Science & Technology |
topic |
olive pomace waste ohmic heating apoptosis Caco-2 cells ROS Science & Technology |
description |
Olive pomace (OP) is the main residue that results from olive oil production. OP is rich in bioactive compounds, including polyphenols, so its use in the treatments of diseases related to oxidative stress, such as cancer, could be considered. The present work aimed to study the biological properties of different OP extracts, obtained by ohmic heating-assisted extraction and conventional heating, using water and 50% ethanol, in the treatment and prevention of colorectal cancer through Caco-2 cell models. Additionally, an in-silico analysis was performed to identify the phenolic intestinal absorption and Caco-2 permeability. The extracts were chemically characterized, and it was found that the Ohmic-hydroethanolic (OH-EtOH) extract had the highest antiproliferative effect, probably due to its higher content of phenolic compounds. The OH-EtOH induced potential modifications in the mitochondrial membrane and led to apoptosis by cell cycle arrest in the G1/S phases with activation of p53 and caspase 3 proteins. In addition, this extract protected the intestine against oxidative stress (ROS) caused by H2O2. Therefore, the bioactive compounds present in OP and recovered by applying a green technology such as ohmic-heating, show promising potential to be used in food, nutraceutical, and biomedical applications, reducing this waste and facilitating the circular economy. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2022-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/77257 |
url |
https://hdl.handle.net/1822/77257 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Quero, Javier; Ballesteros, Lina F.; Ferreira-Santos, P.; Velderrain-Rodriguez, Gustavo R.; Rocha, Cristina M. R.; Pereira, Ricardo N.; Teixeira, José A.; Martin-Belloso, Olga; Osada, Jesús; Rodríguez-Yoldi, María Jesús, Unveiling the antioxidant therapeutic functionality of sustainable olive pomace active ingredients. Antioxidants, 11(5), 828, 2022 2076-3921 10.3390/antiox11050828 https://www.mdpi.com/journal/antioxidants |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI AG |
publisher.none.fl_str_mv |
MDPI AG |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133034779246592 |