Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis

Detalhes bibliográficos
Autor(a) principal: Ferreira, Renato
Data de Publicação: 2023
Outros Autores: Ribeiro, Paulo A., Canário, Adelino V. M., Raposo, Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/164222
Resumo: Funding Information: This research was funded by the project PTDC/FIS-NAN/0909/2014 from Fundação para a Ciência e a Tecnologia (FCT). The authors also thank the research center Grants No. UIDB/FIS/04559/2020 and No. UIDP/FIS/04559/2020 (LIBPhys), from FCT/MCTES, Portugal. Publisher Copyright: © 2023 by the authors.
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spelling Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosiselectronic tongueimpedance spectroscopylayer-by-layer filmspolyelectrolytesprincipal component analysisprostate cancerstanniocalcinSTC1Analytical ChemistryBiotechnologyBiomedical EngineeringInstrumentationEngineering (miscellaneous)Clinical BiochemistrySDG 3 - Good Health and Well-beingFunding Information: This research was funded by the project PTDC/FIS-NAN/0909/2014 from Fundação para a Ciência e a Tecnologia (FCT). The authors also thank the research center Grants No. UIDB/FIS/04559/2020 and No. UIDP/FIS/04559/2020 (LIBPhys), from FCT/MCTES, Portugal. Publisher Copyright: © 2023 by the authors.Prostate cancer is one of the most prevalent tumors in men, accounting for about 7.3% of cancer deaths. Although there are several strategies for diagnosing prostate cancer, these are only accurate when the tumor is already at a very advanced stage, so early diagnosis is essential. Stanniocalcin 1 (STC1) is a secreted glycoprotein, which has been suggested as a tumor marker as its increased expression is associated with the development and/or progression of different types of malignant tumors. In this work, an electronic tongue (ET) prototype, based on a set of four sensors prepared from thin films that included STC1 antibodies for detecting prostate cancer, was developed. In the preparation of the thin films, polyelectrolytes of polyallylamine hydrochloride, polystyrene sulfonate of sodium and polyethyleneimine, and the biomolecules chitosan, protein A, and STC1 antibody were used. These films were deposited on quartz lamellae and on solid supports using layer-on-layer and self-assembly techniques. The deposition of the films was analyzed by ultraviolet-visible spectroscopy, and the detection of STC1 in aqueous solutions of PBS was analyzed by impedance spectroscopy. The impedance data were statistically analyzed using principal component analysis. The ETs formed by the four sensors and the three best sensors could detect the antigen at concentrations in the range from 5 × 10−11 to 5 × 10−4 M. They showed a linear dependence with the logarithm of the antigen concentration and a sensitivity of 5371 ± 820 and 4863 ± 634 per decade of concentration, respectively. Finally, the results allow us to conclude that this prototype can advance to the calibration phase with patient samples.DF – Departamento de FísicaLIBPhys-UNLRUNFerreira, RenatoRibeiro, Paulo A.Canário, Adelino V. M.Raposo, Maria2024-02-27T23:53:12Z2023-11-102023-11-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17application/pdfhttp://hdl.handle.net/10362/164222eng2079-6374PURE: 83890037https://doi.org/10.3390/bios13110981info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:51:18Zoai:run.unl.pt:10362/164222Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T04:00:05.558775Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis
title Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis
spellingShingle Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis
Ferreira, Renato
electronic tongue
impedance spectroscopy
layer-by-layer films
polyelectrolytes
principal component analysis
prostate cancer
stanniocalcin
STC1
Analytical Chemistry
Biotechnology
Biomedical Engineering
Instrumentation
Engineering (miscellaneous)
Clinical Biochemistry
SDG 3 - Good Health and Well-being
title_short Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis
title_full Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis
title_fullStr Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis
title_full_unstemmed Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis
title_sort Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis
author Ferreira, Renato
author_facet Ferreira, Renato
Ribeiro, Paulo A.
Canário, Adelino V. M.
Raposo, Maria
author_role author
author2 Ribeiro, Paulo A.
Canário, Adelino V. M.
Raposo, Maria
author2_role author
author
author
dc.contributor.none.fl_str_mv DF – Departamento de Física
LIBPhys-UNL
RUN
dc.contributor.author.fl_str_mv Ferreira, Renato
Ribeiro, Paulo A.
Canário, Adelino V. M.
Raposo, Maria
dc.subject.por.fl_str_mv electronic tongue
impedance spectroscopy
layer-by-layer films
polyelectrolytes
principal component analysis
prostate cancer
stanniocalcin
STC1
Analytical Chemistry
Biotechnology
Biomedical Engineering
Instrumentation
Engineering (miscellaneous)
Clinical Biochemistry
SDG 3 - Good Health and Well-being
topic electronic tongue
impedance spectroscopy
layer-by-layer films
polyelectrolytes
principal component analysis
prostate cancer
stanniocalcin
STC1
Analytical Chemistry
Biotechnology
Biomedical Engineering
Instrumentation
Engineering (miscellaneous)
Clinical Biochemistry
SDG 3 - Good Health and Well-being
description Funding Information: This research was funded by the project PTDC/FIS-NAN/0909/2014 from Fundação para a Ciência e a Tecnologia (FCT). The authors also thank the research center Grants No. UIDB/FIS/04559/2020 and No. UIDP/FIS/04559/2020 (LIBPhys), from FCT/MCTES, Portugal. Publisher Copyright: © 2023 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-11-10
2023-11-10T00:00:00Z
2024-02-27T23:53:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/164222
url http://hdl.handle.net/10362/164222
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2079-6374
PURE: 83890037
https://doi.org/10.3390/bios13110981
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 17
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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