Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/164222 |
Resumo: | Funding Information: This research was funded by the project PTDC/FIS-NAN/0909/2014 from Fundação para a Ciência e a Tecnologia (FCT). The authors also thank the research center Grants No. UIDB/FIS/04559/2020 and No. UIDP/FIS/04559/2020 (LIBPhys), from FCT/MCTES, Portugal. Publisher Copyright: © 2023 by the authors. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosiselectronic tongueimpedance spectroscopylayer-by-layer filmspolyelectrolytesprincipal component analysisprostate cancerstanniocalcinSTC1Analytical ChemistryBiotechnologyBiomedical EngineeringInstrumentationEngineering (miscellaneous)Clinical BiochemistrySDG 3 - Good Health and Well-beingFunding Information: This research was funded by the project PTDC/FIS-NAN/0909/2014 from Fundação para a Ciência e a Tecnologia (FCT). The authors also thank the research center Grants No. UIDB/FIS/04559/2020 and No. UIDP/FIS/04559/2020 (LIBPhys), from FCT/MCTES, Portugal. Publisher Copyright: © 2023 by the authors.Prostate cancer is one of the most prevalent tumors in men, accounting for about 7.3% of cancer deaths. Although there are several strategies for diagnosing prostate cancer, these are only accurate when the tumor is already at a very advanced stage, so early diagnosis is essential. Stanniocalcin 1 (STC1) is a secreted glycoprotein, which has been suggested as a tumor marker as its increased expression is associated with the development and/or progression of different types of malignant tumors. In this work, an electronic tongue (ET) prototype, based on a set of four sensors prepared from thin films that included STC1 antibodies for detecting prostate cancer, was developed. In the preparation of the thin films, polyelectrolytes of polyallylamine hydrochloride, polystyrene sulfonate of sodium and polyethyleneimine, and the biomolecules chitosan, protein A, and STC1 antibody were used. These films were deposited on quartz lamellae and on solid supports using layer-on-layer and self-assembly techniques. The deposition of the films was analyzed by ultraviolet-visible spectroscopy, and the detection of STC1 in aqueous solutions of PBS was analyzed by impedance spectroscopy. The impedance data were statistically analyzed using principal component analysis. The ETs formed by the four sensors and the three best sensors could detect the antigen at concentrations in the range from 5 × 10−11 to 5 × 10−4 M. They showed a linear dependence with the logarithm of the antigen concentration and a sensitivity of 5371 ± 820 and 4863 ± 634 per decade of concentration, respectively. Finally, the results allow us to conclude that this prototype can advance to the calibration phase with patient samples.DF – Departamento de FísicaLIBPhys-UNLRUNFerreira, RenatoRibeiro, Paulo A.Canário, Adelino V. M.Raposo, Maria2024-02-27T23:53:12Z2023-11-102023-11-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17application/pdfhttp://hdl.handle.net/10362/164222eng2079-6374PURE: 83890037https://doi.org/10.3390/bios13110981info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:51:18Zoai:run.unl.pt:10362/164222Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T04:00:05.558775Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis |
title |
Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis |
spellingShingle |
Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis Ferreira, Renato electronic tongue impedance spectroscopy layer-by-layer films polyelectrolytes principal component analysis prostate cancer stanniocalcin STC1 Analytical Chemistry Biotechnology Biomedical Engineering Instrumentation Engineering (miscellaneous) Clinical Biochemistry SDG 3 - Good Health and Well-being |
title_short |
Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis |
title_full |
Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis |
title_fullStr |
Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis |
title_full_unstemmed |
Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis |
title_sort |
Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis |
author |
Ferreira, Renato |
author_facet |
Ferreira, Renato Ribeiro, Paulo A. Canário, Adelino V. M. Raposo, Maria |
author_role |
author |
author2 |
Ribeiro, Paulo A. Canário, Adelino V. M. Raposo, Maria |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
DF – Departamento de Física LIBPhys-UNL RUN |
dc.contributor.author.fl_str_mv |
Ferreira, Renato Ribeiro, Paulo A. Canário, Adelino V. M. Raposo, Maria |
dc.subject.por.fl_str_mv |
electronic tongue impedance spectroscopy layer-by-layer films polyelectrolytes principal component analysis prostate cancer stanniocalcin STC1 Analytical Chemistry Biotechnology Biomedical Engineering Instrumentation Engineering (miscellaneous) Clinical Biochemistry SDG 3 - Good Health and Well-being |
topic |
electronic tongue impedance spectroscopy layer-by-layer films polyelectrolytes principal component analysis prostate cancer stanniocalcin STC1 Analytical Chemistry Biotechnology Biomedical Engineering Instrumentation Engineering (miscellaneous) Clinical Biochemistry SDG 3 - Good Health and Well-being |
description |
Funding Information: This research was funded by the project PTDC/FIS-NAN/0909/2014 from Fundação para a Ciência e a Tecnologia (FCT). The authors also thank the research center Grants No. UIDB/FIS/04559/2020 and No. UIDP/FIS/04559/2020 (LIBPhys), from FCT/MCTES, Portugal. Publisher Copyright: © 2023 by the authors. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-11-10 2023-11-10T00:00:00Z 2024-02-27T23:53:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/164222 |
url |
http://hdl.handle.net/10362/164222 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2079-6374 PURE: 83890037 https://doi.org/10.3390/bios13110981 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
17 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799138177111293952 |