Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.3390/cancers11101461 |
Resumo: | This work was supported by PhD fellowships from Fundação para a Ciência e a Tecnologia(FCT) to A. Faustino, A. Ferreira and P.C. (PD/BD/105898/2014, PD/BD/135506/2018 and PD/BD/128339/2017, respectively), a post-doctoral fellowship from FCT to C.C. (SFRH/BPD/78561/2011), the FCT Investigator Program to D.C.B. (IF/00501/2014/CP1252/CT0001), and grants from FCT (PTDC/BIM-MEC/4905/2014) and iNOVA4Health - UID/Multi/04462/2013, a program financially supported by FCT/ Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement. |
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7160 |
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Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signalingActin cytoskeletonArl proteinsCancer progressionCell-extracellular matrix adhesionIntegrinsOncologyCancer ResearchSDG 3 - Good Health and Well-beingThis work was supported by PhD fellowships from Fundação para a Ciência e a Tecnologia(FCT) to A. Faustino, A. Ferreira and P.C. (PD/BD/105898/2014, PD/BD/135506/2018 and PD/BD/128339/2017, respectively), a post-doctoral fellowship from FCT to C.C. (SFRH/BPD/78561/2011), the FCT Investigator Program to D.C.B. (IF/00501/2014/CP1252/CT0001), and grants from FCT (PTDC/BIM-MEC/4905/2014) and iNOVA4Health - UID/Multi/04462/2013, a program financially supported by FCT/ Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement.Breast cancer is the first cause of cancer-related mortality among women worldwide, according to the most recent estimates. This mortality is mainly caused by the tumors’ ability to form metastases. Cancer cell migration and invasion are essential for metastasis and rely on the interplay between actin cytoskeleton remodeling and cell adhesion. Therefore, understanding the mechanisms by which cancer cell invasion is controlled may provide new strategies to impair cancer progression. We investigated the role of the ADP-ribosylation factor (Arf)-like (Arl) protein Arl13b in breast cancer cell migration and invasion in vitro, using breast cancer cell lines and in vivo, using mouse orthotopic models. We show that Arl13b silencing inhibits breast cancer cell migration and invasion in vitro, as well as cancer progression in vivo. We also observed that Arl13b is upregulated in breast cancer cell lines and patient tissue samples. Moreover, we found that Arl13b localizes to focal adhesions (FAs) and interacts with β3-integrin. Upon Arl13b silencing, β3-integrin cell surface levels and FA size are increased and integrin-mediated signaling is inhibited. Therefore, we uncover a role for Arl13b in breast cancer cell migration and invasion and provide a new mechanism for how ARL13B can function as an oncogene, through the modulation of integrin-mediated signaling.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNCasalou, CristinaFaustino, AlexandraSilva, FernandaFerreira, Inês C.Vaqueirinho, DanielaFerreira, AndreiaCastanheira, PedroBarona, TeresaRamalho, José S.Serpa, JacintaFélix, AnaBarral, Duarte C.2019-11-15T05:27:27Z2019-10-012019-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.3390/cancers11101461eng2072-6694PURE: 15224183http://www.scopus.com/inward/record.url?scp=85073476079&partnerID=8YFLogxKhttps://doi.org/10.3390/cancers11101461info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:38:55Zoai:run.unl.pt:10362/87326Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:36:44.468631Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling |
title |
Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling |
spellingShingle |
Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling Casalou, Cristina Actin cytoskeleton Arl proteins Cancer progression Cell-extracellular matrix adhesion Integrins Oncology Cancer Research SDG 3 - Good Health and Well-being |
title_short |
Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling |
title_full |
Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling |
title_fullStr |
Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling |
title_full_unstemmed |
Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling |
title_sort |
Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling |
author |
Casalou, Cristina |
author_facet |
Casalou, Cristina Faustino, Alexandra Silva, Fernanda Ferreira, Inês C. Vaqueirinho, Daniela Ferreira, Andreia Castanheira, Pedro Barona, Teresa Ramalho, José S. Serpa, Jacinta Félix, Ana Barral, Duarte C. |
author_role |
author |
author2 |
Faustino, Alexandra Silva, Fernanda Ferreira, Inês C. Vaqueirinho, Daniela Ferreira, Andreia Castanheira, Pedro Barona, Teresa Ramalho, José S. Serpa, Jacinta Félix, Ana Barral, Duarte C. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Centro de Estudos de Doenças Crónicas (CEDOC) NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) RUN |
dc.contributor.author.fl_str_mv |
Casalou, Cristina Faustino, Alexandra Silva, Fernanda Ferreira, Inês C. Vaqueirinho, Daniela Ferreira, Andreia Castanheira, Pedro Barona, Teresa Ramalho, José S. Serpa, Jacinta Félix, Ana Barral, Duarte C. |
dc.subject.por.fl_str_mv |
Actin cytoskeleton Arl proteins Cancer progression Cell-extracellular matrix adhesion Integrins Oncology Cancer Research SDG 3 - Good Health and Well-being |
topic |
Actin cytoskeleton Arl proteins Cancer progression Cell-extracellular matrix adhesion Integrins Oncology Cancer Research SDG 3 - Good Health and Well-being |
description |
This work was supported by PhD fellowships from Fundação para a Ciência e a Tecnologia(FCT) to A. Faustino, A. Ferreira and P.C. (PD/BD/105898/2014, PD/BD/135506/2018 and PD/BD/128339/2017, respectively), a post-doctoral fellowship from FCT to C.C. (SFRH/BPD/78561/2011), the FCT Investigator Program to D.C.B. (IF/00501/2014/CP1252/CT0001), and grants from FCT (PTDC/BIM-MEC/4905/2014) and iNOVA4Health - UID/Multi/04462/2013, a program financially supported by FCT/ Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-15T05:27:27Z 2019-10-01 2019-10-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.3390/cancers11101461 |
url |
https://doi.org/10.3390/cancers11101461 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2072-6694 PURE: 15224183 http://www.scopus.com/inward/record.url?scp=85073476079&partnerID=8YFLogxK https://doi.org/10.3390/cancers11101461 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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