Detection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax, Linnaeus 1758) using non‐lethal sampling methods

Detalhes bibliográficos
Autor(a) principal: Ferreira, Inês Almeida
Data de Publicação: 2019
Outros Autores: Costa, Janina Z., Macchia, Valeria, Thompson, Kimberly Dawn, Baptista, Teresa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/5019
Resumo: One of the major disease threats affecting the Mediterranean aquaculture industry is viral encephalopathy and retinopathy (VER). The target organs for Betanodavirus detection are the brain and eyes, obtained through lethal sampling. This study aimed to evaluate the efficacy and suitability of non‐lethal samples for detecting Betanodavirus in European seabass (Dicentrarchus labrax). European seabass juveniles were infected with Betanodavirus, by either an intramuscular injection or immersion (107 TCID50/ml and 106 TCID50/ml, respectively), and samples collected 7, 15 and 30 days post‐infection (dpi). The brain was collected as a lethal sample, and gills, caudal fin and blood as non‐lethal tissues for detecting Betanodavirus by quantitative reverse transcription PCR (RT‐qPCR). The presence of virus in non‐lethal tissues was inconsistent, with lower viral loads than in the brain. For blood, higher viral loads were detected in intramuscular‐infected fish at 15 dpi until the end of the challenge. Serum antibodies against Betanodavirus were assessed using an enzyme‐linked immunosorbent assay (ELISA). Antibodies were detected as early as 7 dpi, with higher mean antibody titres at 15 and 30 dpi. The presence of Betanodavirus‐specific antibodies indicates that this is a suitable evaluation method for detecting early stages of the infection.
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spelling Detection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax, Linnaeus 1758) using non‐lethal sampling methodsOne of the major disease threats affecting the Mediterranean aquaculture industry is viral encephalopathy and retinopathy (VER). The target organs for Betanodavirus detection are the brain and eyes, obtained through lethal sampling. This study aimed to evaluate the efficacy and suitability of non‐lethal samples for detecting Betanodavirus in European seabass (Dicentrarchus labrax). European seabass juveniles were infected with Betanodavirus, by either an intramuscular injection or immersion (107 TCID50/ml and 106 TCID50/ml, respectively), and samples collected 7, 15 and 30 days post‐infection (dpi). The brain was collected as a lethal sample, and gills, caudal fin and blood as non‐lethal tissues for detecting Betanodavirus by quantitative reverse transcription PCR (RT‐qPCR). The presence of virus in non‐lethal tissues was inconsistent, with lower viral loads than in the brain. For blood, higher viral loads were detected in intramuscular‐infected fish at 15 dpi until the end of the challenge. Serum antibodies against Betanodavirus were assessed using an enzyme‐linked immunosorbent assay (ELISA). Antibodies were detected as early as 7 dpi, with higher mean antibody titres at 15 and 30 dpi. The presence of Betanodavirus‐specific antibodies indicates that this is a suitable evaluation method for detecting early stages of the infection.WileyIC-OnlineFerreira, Inês AlmeidaCosta, Janina Z.Macchia, ValeriaThompson, Kimberly DawnBaptista, Teresa2020-07-17T10:45:42Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/5019engFerreira, I. A., Costa, J. Z., Macchia, V., Thompson, K.D., & Baptista, T. (2019). Detection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax, Linnaeus 1758) using non-lethal sampling methods. Journal of fish diseases, 42(8), 1097–1105. https://doi.org/10.1111/jfd.130150140-777510.1111/jfd.13015metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:50:27Zoai:iconline.ipleiria.pt:10400.8/5019Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:48:43.113324Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Detection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax, Linnaeus 1758) using non‐lethal sampling methods
title Detection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax, Linnaeus 1758) using non‐lethal sampling methods
spellingShingle Detection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax, Linnaeus 1758) using non‐lethal sampling methods
Ferreira, Inês Almeida
title_short Detection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax, Linnaeus 1758) using non‐lethal sampling methods
title_full Detection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax, Linnaeus 1758) using non‐lethal sampling methods
title_fullStr Detection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax, Linnaeus 1758) using non‐lethal sampling methods
title_full_unstemmed Detection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax, Linnaeus 1758) using non‐lethal sampling methods
title_sort Detection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax, Linnaeus 1758) using non‐lethal sampling methods
author Ferreira, Inês Almeida
author_facet Ferreira, Inês Almeida
Costa, Janina Z.
Macchia, Valeria
Thompson, Kimberly Dawn
Baptista, Teresa
author_role author
author2 Costa, Janina Z.
Macchia, Valeria
Thompson, Kimberly Dawn
Baptista, Teresa
author2_role author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Ferreira, Inês Almeida
Costa, Janina Z.
Macchia, Valeria
Thompson, Kimberly Dawn
Baptista, Teresa
description One of the major disease threats affecting the Mediterranean aquaculture industry is viral encephalopathy and retinopathy (VER). The target organs for Betanodavirus detection are the brain and eyes, obtained through lethal sampling. This study aimed to evaluate the efficacy and suitability of non‐lethal samples for detecting Betanodavirus in European seabass (Dicentrarchus labrax). European seabass juveniles were infected with Betanodavirus, by either an intramuscular injection or immersion (107 TCID50/ml and 106 TCID50/ml, respectively), and samples collected 7, 15 and 30 days post‐infection (dpi). The brain was collected as a lethal sample, and gills, caudal fin and blood as non‐lethal tissues for detecting Betanodavirus by quantitative reverse transcription PCR (RT‐qPCR). The presence of virus in non‐lethal tissues was inconsistent, with lower viral loads than in the brain. For blood, higher viral loads were detected in intramuscular‐infected fish at 15 dpi until the end of the challenge. Serum antibodies against Betanodavirus were assessed using an enzyme‐linked immunosorbent assay (ELISA). Antibodies were detected as early as 7 dpi, with higher mean antibody titres at 15 and 30 dpi. The presence of Betanodavirus‐specific antibodies indicates that this is a suitable evaluation method for detecting early stages of the infection.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01T00:00:00Z
2020-07-17T10:45:42Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/5019
url http://hdl.handle.net/10400.8/5019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ferreira, I. A., Costa, J. Z., Macchia, V., Thompson, K.D., & Baptista, T. (2019). Detection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax, Linnaeus 1758) using non-lethal sampling methods. Journal of fish diseases, 42(8), 1097–1105. https://doi.org/10.1111/jfd.13015
0140-7775
10.1111/jfd.13015
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