In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse

Detalhes bibliográficos
Autor(a) principal: Murta, Daniel
Data de Publicação: 2014
Outros Autores: Batista, Marta, Trindade, Alexandre, Silva, Elisabete, Henrique, Domingos, Duarte, António, Lopes-da-Costa, Luís
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/51278
Resumo: Copyright: © 2014 Murta et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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spelling In vivo notch signaling blockade induces abnormal spermatogenesis in the mouseCopyright: © 2014 Murta et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.In a previous study we identified active Notch signaling in key cellular events occurring at adult spermatogenesis. In this study, we evaluated the function of Notch signaling in spermatogenesis through the effects of in vivo Notch blockade. Adult CD1 male mice were either submitted to a long term DAPT (?-secretase inhibitor) or vehicle treatment. Treatment duration was designed to attain one half the time (25 days) or the time (43 days) required to accomplish a complete cycle of spermatogenesis. Blockade of Notch signaling was depicted from decreased transcription of Notch effector genes. Notch signaling blockade disrupted the expression patterns of Notch components in the testis, induced male germ cell fate aberrations, and significantly increased germ cell apoptosis, mainly in the last stages of the spermatogenic cycle, and epididymis spermatozoa morphological defects. These effects were more pronounced following the 43 day than the 25 day DAPT treatment schedule. These results indicate a relevant regulatory role of Notch signaling in mammalian spermatogenesis.We thank the Portuguese Foundation for Science and Technology (FCT) for the financial support (Project PTDC/CVT/105022/2008) and (Project PEst-OE/AGR/U10276/2014). DM is a PhD student supported by grant SFRH/BD/64416/2009 from FCT. MB has a research contract under the grant PTDC/CVT/105022/2008. ES is a Postdoctoral Researcher under the Portuguese Government initiative for research jobs, Ciência 2008-FCT. AT is a Postdoctoral Researcher supported by grant SFRH/BPD/47079/2008 from FCT.PLoSRepositório da Universidade de LisboaMurta, DanielBatista, MartaTrindade, AlexandreSilva, ElisabeteHenrique, DomingosDuarte, AntónioLopes-da-Costa, Luís2022-02-14T14:37:50Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/51278enghttps://journals.plos.org/plosone/1932-620310.1371/journal.pone.0113365info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:55:52Zoai:repositorio.ul.pt:10451/51278Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:02:35.540523Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse
title In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse
spellingShingle In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse
Murta, Daniel
title_short In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse
title_full In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse
title_fullStr In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse
title_full_unstemmed In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse
title_sort In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse
author Murta, Daniel
author_facet Murta, Daniel
Batista, Marta
Trindade, Alexandre
Silva, Elisabete
Henrique, Domingos
Duarte, António
Lopes-da-Costa, Luís
author_role author
author2 Batista, Marta
Trindade, Alexandre
Silva, Elisabete
Henrique, Domingos
Duarte, António
Lopes-da-Costa, Luís
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Murta, Daniel
Batista, Marta
Trindade, Alexandre
Silva, Elisabete
Henrique, Domingos
Duarte, António
Lopes-da-Costa, Luís
description Copyright: © 2014 Murta et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
2022-02-14T14:37:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/51278
url http://hdl.handle.net/10451/51278
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://journals.plos.org/plosone/
1932-6203
10.1371/journal.pone.0113365
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PLoS
publisher.none.fl_str_mv PLoS
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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