In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/51278 |
Resumo: | Copyright: © 2014 Murta et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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In vivo notch signaling blockade induces abnormal spermatogenesis in the mouseCopyright: © 2014 Murta et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.In a previous study we identified active Notch signaling in key cellular events occurring at adult spermatogenesis. In this study, we evaluated the function of Notch signaling in spermatogenesis through the effects of in vivo Notch blockade. Adult CD1 male mice were either submitted to a long term DAPT (?-secretase inhibitor) or vehicle treatment. Treatment duration was designed to attain one half the time (25 days) or the time (43 days) required to accomplish a complete cycle of spermatogenesis. Blockade of Notch signaling was depicted from decreased transcription of Notch effector genes. Notch signaling blockade disrupted the expression patterns of Notch components in the testis, induced male germ cell fate aberrations, and significantly increased germ cell apoptosis, mainly in the last stages of the spermatogenic cycle, and epididymis spermatozoa morphological defects. These effects were more pronounced following the 43 day than the 25 day DAPT treatment schedule. These results indicate a relevant regulatory role of Notch signaling in mammalian spermatogenesis.We thank the Portuguese Foundation for Science and Technology (FCT) for the financial support (Project PTDC/CVT/105022/2008) and (Project PEst-OE/AGR/U10276/2014). DM is a PhD student supported by grant SFRH/BD/64416/2009 from FCT. MB has a research contract under the grant PTDC/CVT/105022/2008. ES is a Postdoctoral Researcher under the Portuguese Government initiative for research jobs, Ciência 2008-FCT. AT is a Postdoctoral Researcher supported by grant SFRH/BPD/47079/2008 from FCT.PLoSRepositório da Universidade de LisboaMurta, DanielBatista, MartaTrindade, AlexandreSilva, ElisabeteHenrique, DomingosDuarte, AntónioLopes-da-Costa, Luís2022-02-14T14:37:50Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/51278enghttps://journals.plos.org/plosone/1932-620310.1371/journal.pone.0113365info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:55:52Zoai:repositorio.ul.pt:10451/51278Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:02:35.540523Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse |
title |
In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse |
spellingShingle |
In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse Murta, Daniel |
title_short |
In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse |
title_full |
In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse |
title_fullStr |
In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse |
title_full_unstemmed |
In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse |
title_sort |
In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse |
author |
Murta, Daniel |
author_facet |
Murta, Daniel Batista, Marta Trindade, Alexandre Silva, Elisabete Henrique, Domingos Duarte, António Lopes-da-Costa, Luís |
author_role |
author |
author2 |
Batista, Marta Trindade, Alexandre Silva, Elisabete Henrique, Domingos Duarte, António Lopes-da-Costa, Luís |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Murta, Daniel Batista, Marta Trindade, Alexandre Silva, Elisabete Henrique, Domingos Duarte, António Lopes-da-Costa, Luís |
description |
Copyright: © 2014 Murta et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z 2022-02-14T14:37:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/51278 |
url |
http://hdl.handle.net/10451/51278 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://journals.plos.org/plosone/ 1932-6203 10.1371/journal.pone.0113365 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
PLoS |
publisher.none.fl_str_mv |
PLoS |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799134576000368640 |