The splicing factor XAB2 interacts with ERCC1-XPF and XPG for R-loop processing

Detalhes bibliográficos
Autor(a) principal: Goulielmaki, Evi
Data de Publicação: 2021
Outros Autores: Tsekrekou, Maria, Batsiotos, Nikos, Ascensão-Ferreira, Mariana, Ledaki, Eleftheria, Stratigi, Kalliopi, Chatzinikolaou, Georgia, Topalis, Pantelis, Kosteas, Theodore, Altmüller, Janine, Demmers, Jeroen A., Barbosa-Morais, Nuno, Garinis, George A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/48990
Resumo: © The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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spelling The splicing factor XAB2 interacts with ERCC1-XPF and XPG for R-loop processing© The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.RNA splicing, transcription and the DNA damage response are intriguingly linked in mammals but the underlying mechanisms remain poorly understood. Using an in vivo biotinylation tagging approach in mice, we show that the splicing factor XAB2 interacts with the core spliceosome and that it binds to spliceosomal U4 and U6 snRNAs and pre-mRNAs in developing livers. XAB2 depletion leads to aberrant intron retention, R-loop formation and DNA damage in cells. Studies in illudin S-treated cells and Csbm/m developing livers reveal that transcription-blocking DNA lesions trigger the release of XAB2 from all RNA targets tested. Immunoprecipitation studies reveal that XAB2 interacts with ERCC1-XPF and XPG endonucleases outside nucleotide excision repair and that the trimeric protein complex binds RNA:DNA hybrids under conditions that favor the formation of R-loops. Thus, XAB2 functionally links the spliceosomal response to DNA damage with R-loop processing with important ramifications for transcription-coupled DNA repair disorders.The Horizon 2020 ERC Consolidator grant “DeFiNER” (GA64663), the Horizon 2020 Marie Curie ITNs “Chromatin3D” (GA622934), “aDDRess” (GA812829) and “HealthAge” “(GA812830), the Hellenic Foundation for Research and Innovation (HFRI) and the General Secretariat for Research and Technology (GSRT) under grant agreement HFRI-1059 and HFRI-FM17-631 and the “Fondation Santé” grant supported this work. G.C. is supported by the IKY postdoctoral research fellowship program (MIS: 5001552), co-financed by the European Social Fund- ESF and the Greek government. N.L.B.-M. is supported by an EMBO Installation Grant (3057) and an Investigador FCT (Fundação para a Ciência e a Tecnologia) Starting Grant (IF/00595/2014); M.A.-F. is supported by an FCT PhD fellowship (PD/BD/128283/2017) and Fundação AstraZeneca.Springer NatureRepositório da Universidade de LisboaGoulielmaki, EviTsekrekou, MariaBatsiotos, NikosAscensão-Ferreira, MarianaLedaki, EleftheriaStratigi, KalliopiChatzinikolaou, GeorgiaTopalis, PantelisKosteas, TheodoreAltmüller, JanineDemmers, Jeroen A.Barbosa-Morais, NunoGarinis, George A.2021-07-19T12:12:57Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/48990engNat Commun. 2021 May 26;12(1):3153.10.1038/s41467-021-23505-12041-1723info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:52:11Zoai:repositorio.ul.pt:10451/48990Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:00:31.709447Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The splicing factor XAB2 interacts with ERCC1-XPF and XPG for R-loop processing
title The splicing factor XAB2 interacts with ERCC1-XPF and XPG for R-loop processing
spellingShingle The splicing factor XAB2 interacts with ERCC1-XPF and XPG for R-loop processing
Goulielmaki, Evi
title_short The splicing factor XAB2 interacts with ERCC1-XPF and XPG for R-loop processing
title_full The splicing factor XAB2 interacts with ERCC1-XPF and XPG for R-loop processing
title_fullStr The splicing factor XAB2 interacts with ERCC1-XPF and XPG for R-loop processing
title_full_unstemmed The splicing factor XAB2 interacts with ERCC1-XPF and XPG for R-loop processing
title_sort The splicing factor XAB2 interacts with ERCC1-XPF and XPG for R-loop processing
author Goulielmaki, Evi
author_facet Goulielmaki, Evi
Tsekrekou, Maria
Batsiotos, Nikos
Ascensão-Ferreira, Mariana
Ledaki, Eleftheria
Stratigi, Kalliopi
Chatzinikolaou, Georgia
Topalis, Pantelis
Kosteas, Theodore
Altmüller, Janine
Demmers, Jeroen A.
Barbosa-Morais, Nuno
Garinis, George A.
author_role author
author2 Tsekrekou, Maria
Batsiotos, Nikos
Ascensão-Ferreira, Mariana
Ledaki, Eleftheria
Stratigi, Kalliopi
Chatzinikolaou, Georgia
Topalis, Pantelis
Kosteas, Theodore
Altmüller, Janine
Demmers, Jeroen A.
Barbosa-Morais, Nuno
Garinis, George A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Goulielmaki, Evi
Tsekrekou, Maria
Batsiotos, Nikos
Ascensão-Ferreira, Mariana
Ledaki, Eleftheria
Stratigi, Kalliopi
Chatzinikolaou, Georgia
Topalis, Pantelis
Kosteas, Theodore
Altmüller, Janine
Demmers, Jeroen A.
Barbosa-Morais, Nuno
Garinis, George A.
description © The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-19T12:12:57Z
2021
2021-01-01T00:00:00Z
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dc.relation.none.fl_str_mv Nat Commun. 2021 May 26;12(1):3153.
10.1038/s41467-021-23505-1
2041-1723
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