Electrochemical genosensor for the detection of Dynophisis spp. dinoflagellates
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/24768 |
Resumo: | Harmful algal blooms (HABs) pose a significant threat to the environment and public health. To minimize their impact, it is necessary to develop technological devices towards their fast and precise detection. Therefore, the aim of this work was to develop an analytical approach based on an electrochemical genosensor device in order to create a low-cost platform to monitor the presence of dinoflagellates from the genus Dinophysis. The design of this deoxyribonucleic acid (DNA)-based sensor consists in three steps: (i) Sensing phase, where a mixed self-assembled monolayer composed by a linear DNA-capture probe and mercaptohexanol onto the electrode’s surface is generated; (ii) Hybridization of complementary DNA sequence through a “sandwich” hybridization assay; and (iii) Electrochemical detection by chronoamperometry, taking advantage of an enzymatic scheme to amplify the signal. The best analytical conditions were used to study the relationship between the electrochemical signal and the DNA-target concentration, to produce the best electrochemical genosensor device. Polymerase chain reaction (PCR) was used for validation of the electrochemical genosensor. These advancements contribute to the ongoing efforts in environment and public health’s protection by providing effective tools for mitigating the risks of HABs. Further research and implementation of these methods hold great potential in ensuring the safety and sustainability of aquatic ecosystems. |
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Electrochemical genosensor for the detection of Dynophisis spp. dinoflagellatesDinoflagellatesDynophisis spp.GenosensorMolecular biologyHarmful algal blooms (HABs) pose a significant threat to the environment and public health. To minimize their impact, it is necessary to develop technological devices towards their fast and precise detection. Therefore, the aim of this work was to develop an analytical approach based on an electrochemical genosensor device in order to create a low-cost platform to monitor the presence of dinoflagellates from the genus Dinophysis. The design of this deoxyribonucleic acid (DNA)-based sensor consists in three steps: (i) Sensing phase, where a mixed self-assembled monolayer composed by a linear DNA-capture probe and mercaptohexanol onto the electrode’s surface is generated; (ii) Hybridization of complementary DNA sequence through a “sandwich” hybridization assay; and (iii) Electrochemical detection by chronoamperometry, taking advantage of an enzymatic scheme to amplify the signal. The best analytical conditions were used to study the relationship between the electrochemical signal and the DNA-target concentration, to produce the best electrochemical genosensor device. Polymerase chain reaction (PCR) was used for validation of the electrochemical genosensor. These advancements contribute to the ongoing efforts in environment and public health’s protection by providing effective tools for mitigating the risks of HABs. Further research and implementation of these methods hold great potential in ensuring the safety and sustainability of aquatic ecosystems.Santos, MarleneBarroso, FátimaSilva, NádiaVieira, MónicaRepositório Científico do Instituto Politécnico do PortoPereira, Eduarda Almeida Rufo2023-11-212026-11-21T00:00:00Z2023-11-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.22/24768TID:203472780enginfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-31T01:51:04Zoai:recipp.ipp.pt:10400.22/24768Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:59:08.324704Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Electrochemical genosensor for the detection of Dynophisis spp. dinoflagellates |
title |
Electrochemical genosensor for the detection of Dynophisis spp. dinoflagellates |
spellingShingle |
Electrochemical genosensor for the detection of Dynophisis spp. dinoflagellates Pereira, Eduarda Almeida Rufo Dinoflagellates Dynophisis spp. Genosensor Molecular biology |
title_short |
Electrochemical genosensor for the detection of Dynophisis spp. dinoflagellates |
title_full |
Electrochemical genosensor for the detection of Dynophisis spp. dinoflagellates |
title_fullStr |
Electrochemical genosensor for the detection of Dynophisis spp. dinoflagellates |
title_full_unstemmed |
Electrochemical genosensor for the detection of Dynophisis spp. dinoflagellates |
title_sort |
Electrochemical genosensor for the detection of Dynophisis spp. dinoflagellates |
author |
Pereira, Eduarda Almeida Rufo |
author_facet |
Pereira, Eduarda Almeida Rufo |
author_role |
author |
dc.contributor.none.fl_str_mv |
Santos, Marlene Barroso, Fátima Silva, Nádia Vieira, Mónica Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Pereira, Eduarda Almeida Rufo |
dc.subject.por.fl_str_mv |
Dinoflagellates Dynophisis spp. Genosensor Molecular biology |
topic |
Dinoflagellates Dynophisis spp. Genosensor Molecular biology |
description |
Harmful algal blooms (HABs) pose a significant threat to the environment and public health. To minimize their impact, it is necessary to develop technological devices towards their fast and precise detection. Therefore, the aim of this work was to develop an analytical approach based on an electrochemical genosensor device in order to create a low-cost platform to monitor the presence of dinoflagellates from the genus Dinophysis. The design of this deoxyribonucleic acid (DNA)-based sensor consists in three steps: (i) Sensing phase, where a mixed self-assembled monolayer composed by a linear DNA-capture probe and mercaptohexanol onto the electrode’s surface is generated; (ii) Hybridization of complementary DNA sequence through a “sandwich” hybridization assay; and (iii) Electrochemical detection by chronoamperometry, taking advantage of an enzymatic scheme to amplify the signal. The best analytical conditions were used to study the relationship between the electrochemical signal and the DNA-target concentration, to produce the best electrochemical genosensor device. Polymerase chain reaction (PCR) was used for validation of the electrochemical genosensor. These advancements contribute to the ongoing efforts in environment and public health’s protection by providing effective tools for mitigating the risks of HABs. Further research and implementation of these methods hold great potential in ensuring the safety and sustainability of aquatic ecosystems. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-11-21 2023-11-21T00:00:00Z 2026-11-21T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/24768 TID:203472780 |
url |
http://hdl.handle.net/10400.22/24768 |
identifier_str_mv |
TID:203472780 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137075406045184 |