Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/18944 |
Resumo: | This work addresses the development of a disposable electrochemical genosensor for the detection of the toxic dinoflagellate, Alexandrium minutum. Analyzing public databases, a specific 70 bp DNA probe, targeting A. minutum, was selected and designed. The genosensor methodology implied the immobilization of a A. minutum-specific DNA-capture probe onto screen-printed gold electrodes (SPGE). To improve both the selectivity and to avoid strong secondary structures, that could hinder the hybridization efficiency, a sandwich format of the A. minutum gene was designed using a fluorescein isothiocyanate-labelled signaling DNA probe and enzymatic amplification of the electrochemical signal. Using this electrochemical genosensor, a oncentration range from 0.12 to 1.0 nM, a LD of 24.78 pM with a RSD <5.2% was determined. The genosensor was successfully applied to the selective analysis of the targeted A. minutum specific region denatured genomic DNA extracted from toxic dinoflagellates present in the Atlantic Ocean. |
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Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellatesAlexandrium minutumDinoflagellateChronoamperometryElectrochemical genosensorSandwich format hybridizationThis work addresses the development of a disposable electrochemical genosensor for the detection of the toxic dinoflagellate, Alexandrium minutum. Analyzing public databases, a specific 70 bp DNA probe, targeting A. minutum, was selected and designed. The genosensor methodology implied the immobilization of a A. minutum-specific DNA-capture probe onto screen-printed gold electrodes (SPGE). To improve both the selectivity and to avoid strong secondary structures, that could hinder the hybridization efficiency, a sandwich format of the A. minutum gene was designed using a fluorescein isothiocyanate-labelled signaling DNA probe and enzymatic amplification of the electrochemical signal. Using this electrochemical genosensor, a oncentration range from 0.12 to 1.0 nM, a LD of 24.78 pM with a RSD <5.2% was determined. The genosensor was successfully applied to the selective analysis of the targeted A. minutum specific region denatured genomic DNA extracted from toxic dinoflagellates present in the Atlantic Ocean.ElsevierRepositório Científico do Instituto Politécnico do PortoMorais, Stephanie L.Barros, PiedadeSantos, MarleneDelerue-Matos, CristinaGomes, Andreia C.Barroso, M. Fátima2021-11-24T16:39:44Z2021-01-152021-01-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/18944engMorais, S. L., Barros, P., Santos, M., Delerue-Matos, C., Gomes, A. C., & Fátima Barroso, M. (2021). Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates. Talanta, 222, 121416. https://doi.org/10.1016/j.talanta.2020.1214160039-914010.1016/j.talanta.2020.121416metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:12:13Zoai:recipp.ipp.pt:10400.22/18944Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:38:59.665946Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates |
title |
Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates |
spellingShingle |
Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates Morais, Stephanie L. Alexandrium minutum Dinoflagellate Chronoamperometry Electrochemical genosensor Sandwich format hybridization |
title_short |
Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates |
title_full |
Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates |
title_fullStr |
Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates |
title_full_unstemmed |
Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates |
title_sort |
Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates |
author |
Morais, Stephanie L. |
author_facet |
Morais, Stephanie L. Barros, Piedade Santos, Marlene Delerue-Matos, Cristina Gomes, Andreia C. Barroso, M. Fátima |
author_role |
author |
author2 |
Barros, Piedade Santos, Marlene Delerue-Matos, Cristina Gomes, Andreia C. Barroso, M. Fátima |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Morais, Stephanie L. Barros, Piedade Santos, Marlene Delerue-Matos, Cristina Gomes, Andreia C. Barroso, M. Fátima |
dc.subject.por.fl_str_mv |
Alexandrium minutum Dinoflagellate Chronoamperometry Electrochemical genosensor Sandwich format hybridization |
topic |
Alexandrium minutum Dinoflagellate Chronoamperometry Electrochemical genosensor Sandwich format hybridization |
description |
This work addresses the development of a disposable electrochemical genosensor for the detection of the toxic dinoflagellate, Alexandrium minutum. Analyzing public databases, a specific 70 bp DNA probe, targeting A. minutum, was selected and designed. The genosensor methodology implied the immobilization of a A. minutum-specific DNA-capture probe onto screen-printed gold electrodes (SPGE). To improve both the selectivity and to avoid strong secondary structures, that could hinder the hybridization efficiency, a sandwich format of the A. minutum gene was designed using a fluorescein isothiocyanate-labelled signaling DNA probe and enzymatic amplification of the electrochemical signal. Using this electrochemical genosensor, a oncentration range from 0.12 to 1.0 nM, a LD of 24.78 pM with a RSD <5.2% was determined. The genosensor was successfully applied to the selective analysis of the targeted A. minutum specific region denatured genomic DNA extracted from toxic dinoflagellates present in the Atlantic Ocean. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-24T16:39:44Z 2021-01-15 2021-01-15T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/18944 |
url |
http://hdl.handle.net/10400.22/18944 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Morais, S. L., Barros, P., Santos, M., Delerue-Matos, C., Gomes, A. C., & Fátima Barroso, M. (2021). Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates. Talanta, 222, 121416. https://doi.org/10.1016/j.talanta.2020.121416 0039-9140 10.1016/j.talanta.2020.121416 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
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metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131477978382336 |