Efficiency of purification methods on the recovery of exopolysaccharides from fermentation media
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/37103 |
Resumo: | De-Man Rogosa and Sharpe (MRS) is a complex medium commonly used to obtain exopolysaccharides (EPS) from lactic acid bacteria. However, the various nutrients (carbon and nitrogen sources) of media and supplements added to enhance the bacterial growth and EPS production, may interfere with the purification of the extracts resulting in an over-estimation of the EPS and erroneous structural assignments. The efficiency of trichloroacetic acid (TCA)/pronase and 5-sulfosalicylic acid - SSA methods was evaluated. In addition, the interference of the major MRS broth components as well as lactose was evaluated using xanthan gum as model control EPS. It was concluded that MRS medium is a major source of interfering compounds in the quantification of EPS, mainly glucose-rich material and to a lesser extent mannoproteins from yeast extract. This effect was found to be potentiated by the presence of lactose. TCA/pronase method was shown to be more efficient in eliminating interferents. |
id |
RCAP_9465e4ae91598171079e622eb911389e |
---|---|
oai_identifier_str |
oai:ria.ua.pt:10773/37103 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Efficiency of purification methods on the recovery of exopolysaccharides from fermentation mediaExopolysaccharidesTrichloroacetic acid/pronase5-sulfosalicylic acidMRS brothYeast extractXanthan gumLactoseDe-Man Rogosa and Sharpe (MRS) is a complex medium commonly used to obtain exopolysaccharides (EPS) from lactic acid bacteria. However, the various nutrients (carbon and nitrogen sources) of media and supplements added to enhance the bacterial growth and EPS production, may interfere with the purification of the extracts resulting in an over-estimation of the EPS and erroneous structural assignments. The efficiency of trichloroacetic acid (TCA)/pronase and 5-sulfosalicylic acid - SSA methods was evaluated. In addition, the interference of the major MRS broth components as well as lactose was evaluated using xanthan gum as model control EPS. It was concluded that MRS medium is a major source of interfering compounds in the quantification of EPS, mainly glucose-rich material and to a lesser extent mannoproteins from yeast extract. This effect was found to be potentiated by the presence of lactose. TCA/pronase method was shown to be more efficient in eliminating interferents.Elsevier2023-04-17T10:57:27Z2020-03-01T00:00:00Z2020-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/37103eng0144-861710.1016/j.carbpol.2019.115703Pintado, Ana I. E.Ferreira, José A.Pintado, Manuela M. E.Gomes, Ana M. P.Malcata, F. XavierCoimbra, Manuel A.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:11:32Zoai:ria.ua.pt:10773/37103Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:07:44.802831Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Efficiency of purification methods on the recovery of exopolysaccharides from fermentation media |
title |
Efficiency of purification methods on the recovery of exopolysaccharides from fermentation media |
spellingShingle |
Efficiency of purification methods on the recovery of exopolysaccharides from fermentation media Pintado, Ana I. E. Exopolysaccharides Trichloroacetic acid/pronase 5-sulfosalicylic acid MRS broth Yeast extract Xanthan gum Lactose |
title_short |
Efficiency of purification methods on the recovery of exopolysaccharides from fermentation media |
title_full |
Efficiency of purification methods on the recovery of exopolysaccharides from fermentation media |
title_fullStr |
Efficiency of purification methods on the recovery of exopolysaccharides from fermentation media |
title_full_unstemmed |
Efficiency of purification methods on the recovery of exopolysaccharides from fermentation media |
title_sort |
Efficiency of purification methods on the recovery of exopolysaccharides from fermentation media |
author |
Pintado, Ana I. E. |
author_facet |
Pintado, Ana I. E. Ferreira, José A. Pintado, Manuela M. E. Gomes, Ana M. P. Malcata, F. Xavier Coimbra, Manuel A. |
author_role |
author |
author2 |
Ferreira, José A. Pintado, Manuela M. E. Gomes, Ana M. P. Malcata, F. Xavier Coimbra, Manuel A. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pintado, Ana I. E. Ferreira, José A. Pintado, Manuela M. E. Gomes, Ana M. P. Malcata, F. Xavier Coimbra, Manuel A. |
dc.subject.por.fl_str_mv |
Exopolysaccharides Trichloroacetic acid/pronase 5-sulfosalicylic acid MRS broth Yeast extract Xanthan gum Lactose |
topic |
Exopolysaccharides Trichloroacetic acid/pronase 5-sulfosalicylic acid MRS broth Yeast extract Xanthan gum Lactose |
description |
De-Man Rogosa and Sharpe (MRS) is a complex medium commonly used to obtain exopolysaccharides (EPS) from lactic acid bacteria. However, the various nutrients (carbon and nitrogen sources) of media and supplements added to enhance the bacterial growth and EPS production, may interfere with the purification of the extracts resulting in an over-estimation of the EPS and erroneous structural assignments. The efficiency of trichloroacetic acid (TCA)/pronase and 5-sulfosalicylic acid - SSA methods was evaluated. In addition, the interference of the major MRS broth components as well as lactose was evaluated using xanthan gum as model control EPS. It was concluded that MRS medium is a major source of interfering compounds in the quantification of EPS, mainly glucose-rich material and to a lesser extent mannoproteins from yeast extract. This effect was found to be potentiated by the presence of lactose. TCA/pronase method was shown to be more efficient in eliminating interferents. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-03-01T00:00:00Z 2020-03-01 2023-04-17T10:57:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/37103 |
url |
http://hdl.handle.net/10773/37103 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0144-8617 10.1016/j.carbpol.2019.115703 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799137731711860736 |