Microencapsulated chitosan–dextran sulfate nanoparticles for controled delivery of bioactive molecules and cells in bone regeneration

Detalhes bibliográficos
Autor(a) principal: Valente, Joana
Data de Publicação: 2013
Outros Autores: Gaspar, Vítor Manuel Abreu, Antunes, Bernardo Paiva, Coutinho, Paula Isabel Teixeira Gonçalves, Correia, Ilídio Joaquim Sobreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.6/4647
Resumo: This research work aimed to synthesize and characterize a novel dual delivery system comprised of BSA-loaded in chitosan–dextran sulfate nanoparticulated carriers and mesenchymal stem cells that are encapsulated into alginate microparticles. The physicochemical and biological characteristics of this novel system, such as, morphology, release, swelling, and cytotoxicity were thoroughly characterized. The results obtained from confocal microscopy demonstrate that chitosan–dextran sulfate nanoparticles and cells are fully encapsulated within alginate microparticles, and spatially dispersed in the microparticle matrix. Moreover, scanning electron microscopy images revealed that these micro-sized carriers possess a rough surface, an important parameter that also promoted proper cell migration and adhesion. Notably, the incorporation of BSA in this duplex nano-micro delivery system extended its release profile throughout time, in comparison with microparticles alone, whilst not eliciting any cell damage. Taken together, these findings suggest that this dual carrier is a versatile delivery system with potential for a spatiotemporally controlled release of bioactive molecules and cells.
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spelling Microencapsulated chitosan–dextran sulfate nanoparticles for controled delivery of bioactive molecules and cells in bone regenerationAlginate microparticlesChitosan-dextran sulfate nanoparticlesProtein and cell deliveryThis research work aimed to synthesize and characterize a novel dual delivery system comprised of BSA-loaded in chitosan–dextran sulfate nanoparticulated carriers and mesenchymal stem cells that are encapsulated into alginate microparticles. The physicochemical and biological characteristics of this novel system, such as, morphology, release, swelling, and cytotoxicity were thoroughly characterized. The results obtained from confocal microscopy demonstrate that chitosan–dextran sulfate nanoparticles and cells are fully encapsulated within alginate microparticles, and spatially dispersed in the microparticle matrix. Moreover, scanning electron microscopy images revealed that these micro-sized carriers possess a rough surface, an important parameter that also promoted proper cell migration and adhesion. Notably, the incorporation of BSA in this duplex nano-micro delivery system extended its release profile throughout time, in comparison with microparticles alone, whilst not eliciting any cell damage. Taken together, these findings suggest that this dual carrier is a versatile delivery system with potential for a spatiotemporally controlled release of bioactive molecules and cells.ElsevieruBibliorumValente, JoanaGaspar, Vítor Manuel AbreuAntunes, Bernardo PaivaCoutinho, Paula Isabel Teixeira GonçalvesCorreia, Ilídio Joaquim Sobreira2018-03-20T10:02:47Z2013-01-082013-01-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4647engValente, J., Gaspar, V.M., Antunes, B., Coutinho, P. e Correia, I.J. (2013) “Microencapsulated chitosan-dextran sulfate nanoparticles for controlled delivery of bioactive molecules and cells in bone regeneration”, Polymer, Vol. 54(1), pp. 5-1510.1016/j.polymer.2012.10.032metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-15T09:41:50Zoai:ubibliorum.ubi.pt:10400.6/4647Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:45:42.458289Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Microencapsulated chitosan–dextran sulfate nanoparticles for controled delivery of bioactive molecules and cells in bone regeneration
title Microencapsulated chitosan–dextran sulfate nanoparticles for controled delivery of bioactive molecules and cells in bone regeneration
spellingShingle Microencapsulated chitosan–dextran sulfate nanoparticles for controled delivery of bioactive molecules and cells in bone regeneration
Valente, Joana
Alginate microparticles
Chitosan-dextran sulfate nanoparticles
Protein and cell delivery
title_short Microencapsulated chitosan–dextran sulfate nanoparticles for controled delivery of bioactive molecules and cells in bone regeneration
title_full Microencapsulated chitosan–dextran sulfate nanoparticles for controled delivery of bioactive molecules and cells in bone regeneration
title_fullStr Microencapsulated chitosan–dextran sulfate nanoparticles for controled delivery of bioactive molecules and cells in bone regeneration
title_full_unstemmed Microencapsulated chitosan–dextran sulfate nanoparticles for controled delivery of bioactive molecules and cells in bone regeneration
title_sort Microencapsulated chitosan–dextran sulfate nanoparticles for controled delivery of bioactive molecules and cells in bone regeneration
author Valente, Joana
author_facet Valente, Joana
Gaspar, Vítor Manuel Abreu
Antunes, Bernardo Paiva
Coutinho, Paula Isabel Teixeira Gonçalves
Correia, Ilídio Joaquim Sobreira
author_role author
author2 Gaspar, Vítor Manuel Abreu
Antunes, Bernardo Paiva
Coutinho, Paula Isabel Teixeira Gonçalves
Correia, Ilídio Joaquim Sobreira
author2_role author
author
author
author
dc.contributor.none.fl_str_mv uBibliorum
dc.contributor.author.fl_str_mv Valente, Joana
Gaspar, Vítor Manuel Abreu
Antunes, Bernardo Paiva
Coutinho, Paula Isabel Teixeira Gonçalves
Correia, Ilídio Joaquim Sobreira
dc.subject.por.fl_str_mv Alginate microparticles
Chitosan-dextran sulfate nanoparticles
Protein and cell delivery
topic Alginate microparticles
Chitosan-dextran sulfate nanoparticles
Protein and cell delivery
description This research work aimed to synthesize and characterize a novel dual delivery system comprised of BSA-loaded in chitosan–dextran sulfate nanoparticulated carriers and mesenchymal stem cells that are encapsulated into alginate microparticles. The physicochemical and biological characteristics of this novel system, such as, morphology, release, swelling, and cytotoxicity were thoroughly characterized. The results obtained from confocal microscopy demonstrate that chitosan–dextran sulfate nanoparticles and cells are fully encapsulated within alginate microparticles, and spatially dispersed in the microparticle matrix. Moreover, scanning electron microscopy images revealed that these micro-sized carriers possess a rough surface, an important parameter that also promoted proper cell migration and adhesion. Notably, the incorporation of BSA in this duplex nano-micro delivery system extended its release profile throughout time, in comparison with microparticles alone, whilst not eliciting any cell damage. Taken together, these findings suggest that this dual carrier is a versatile delivery system with potential for a spatiotemporally controlled release of bioactive molecules and cells.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-08
2013-01-08T00:00:00Z
2018-03-20T10:02:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.6/4647
url http://hdl.handle.net/10400.6/4647
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Valente, J., Gaspar, V.M., Antunes, B., Coutinho, P. e Correia, I.J. (2013) “Microencapsulated chitosan-dextran sulfate nanoparticles for controlled delivery of bioactive molecules and cells in bone regeneration”, Polymer, Vol. 54(1), pp. 5-15
10.1016/j.polymer.2012.10.032
dc.rights.driver.fl_str_mv metadata only access
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rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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