Separation and identification of neutral cereal lipids by normal phase

Detalhes bibliográficos
Autor(a) principal: Rocha, João M.
Data de Publicação: 2010
Outros Autores: Kalo, Paavo J., Ollilainen, Velimatti, Malcata, F. Xavier
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/2657
Resumo: A novel method was developed for the analysis of molecular species in neutral lipid classes, using separation by normal phase high-performance liquid chromatography, followed by detection by evaporative light-scattering and electrospray ionization tandem mass spectrometry. Monoacid standards, i.e. sterol esters, triacylglycerols, fatty acids, diacylglycerols, free sterols and monoacylglycerols, were separated to baseline on microbore 3 m-silica gel columns. Complete or partial separation of molecular species in each lipid class permitted identification by automatic tandem mass spectrometry of ammonium adducts, produced via positive electrospray ionization. After optimization of the method, separation and identification of molecular species of various lipid classes was comprehensively tested by analysis of neutral lipids from the free lipid extract of maize flour.
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spelling Separation and identification of neutral cereal lipids by normal phaseSimple lipidsAcylglycerolsFree sterolsFatty acidsNormal phase liquid chromatographyEvaporative light-scattering detectionPositive electrospray ionization tandem mass spectrometryA novel method was developed for the analysis of molecular species in neutral lipid classes, using separation by normal phase high-performance liquid chromatography, followed by detection by evaporative light-scattering and electrospray ionization tandem mass spectrometry. Monoacid standards, i.e. sterol esters, triacylglycerols, fatty acids, diacylglycerols, free sterols and monoacylglycerols, were separated to baseline on microbore 3 m-silica gel columns. Complete or partial separation of molecular species in each lipid class permitted identification by automatic tandem mass spectrometry of ammonium adducts, produced via positive electrospray ionization. After optimization of the method, separation and identification of molecular species of various lipid classes was comprehensively tested by analysis of neutral lipids from the free lipid extract of maize flour.ElsevierVeritati - Repositório Institucional da Universidade Católica PortuguesaRocha, João M.Kalo, Paavo J.Ollilainen, VelimattiMalcata, F. Xavier2010-10-04T11:20:24Z20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/2657eng"Journal of Chromatography A". ISSN 0021-9673. 1217: 28 (2010) 3013–30250021-967310.1016/j.chroma.2010.02.0341873-3778WOS:000277373800003info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:09:08ZPortal AgregadorONG
dc.title.none.fl_str_mv Separation and identification of neutral cereal lipids by normal phase
title Separation and identification of neutral cereal lipids by normal phase
spellingShingle Separation and identification of neutral cereal lipids by normal phase
Rocha, João M.
Simple lipids
Acylglycerols
Free sterols
Fatty acids
Normal phase liquid chromatography
Evaporative light-scattering detection
Positive electrospray ionization tandem mass spectrometry
title_short Separation and identification of neutral cereal lipids by normal phase
title_full Separation and identification of neutral cereal lipids by normal phase
title_fullStr Separation and identification of neutral cereal lipids by normal phase
title_full_unstemmed Separation and identification of neutral cereal lipids by normal phase
title_sort Separation and identification of neutral cereal lipids by normal phase
author Rocha, João M.
author_facet Rocha, João M.
Kalo, Paavo J.
Ollilainen, Velimatti
Malcata, F. Xavier
author_role author
author2 Kalo, Paavo J.
Ollilainen, Velimatti
Malcata, F. Xavier
author2_role author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Rocha, João M.
Kalo, Paavo J.
Ollilainen, Velimatti
Malcata, F. Xavier
dc.subject.por.fl_str_mv Simple lipids
Acylglycerols
Free sterols
Fatty acids
Normal phase liquid chromatography
Evaporative light-scattering detection
Positive electrospray ionization tandem mass spectrometry
topic Simple lipids
Acylglycerols
Free sterols
Fatty acids
Normal phase liquid chromatography
Evaporative light-scattering detection
Positive electrospray ionization tandem mass spectrometry
description A novel method was developed for the analysis of molecular species in neutral lipid classes, using separation by normal phase high-performance liquid chromatography, followed by detection by evaporative light-scattering and electrospray ionization tandem mass spectrometry. Monoacid standards, i.e. sterol esters, triacylglycerols, fatty acids, diacylglycerols, free sterols and monoacylglycerols, were separated to baseline on microbore 3 m-silica gel columns. Complete or partial separation of molecular species in each lipid class permitted identification by automatic tandem mass spectrometry of ammonium adducts, produced via positive electrospray ionization. After optimization of the method, separation and identification of molecular species of various lipid classes was comprehensively tested by analysis of neutral lipids from the free lipid extract of maize flour.
publishDate 2010
dc.date.none.fl_str_mv 2010-10-04T11:20:24Z
2010
2010-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/2657
url http://hdl.handle.net/10400.14/2657
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Journal of Chromatography A". ISSN 0021-9673. 1217: 28 (2010) 3013–3025
0021-9673
10.1016/j.chroma.2010.02.034
1873-3778
WOS:000277373800003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv
repository.mail.fl_str_mv
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