A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans
Autor(a) principal: | |
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Data de Publicação: | 1996 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.1074/jbc.271.19.11126 |
Resumo: | Wellcome Trust |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificansBiochemistryMolecular BiologyCell BiologyWellcome TrustThe diheme cytochrome e peroxidase from Paracoccus denitrificans was modified with the histidine-specific reagent diethyl pyrocarbonate. At low excess of reagent, 1 mol of histidine was modified in the oxidized enzyme, and modification was associated with loss of the ability to form the active state. With time, the modification reversed, and the ability to form the active state was recovered. The agreement between the spectrophotometric measurement of histidine modification and radioactive incorporation using a radiolabeled reagent indicated little modification of other amino acids. However, the reversal of histidine modification observed spectrophotometrically was not matched by loss of radioactivity, and we propose a slow transfer of the ethoxyformyl group to an unidentified amino acid. The presence of CN- bound to the active peroxidatic site of the enzyme led to complete protection of the essential histidine from modification. Limited subtilisin treatment of the native enzyme followed by tryptic digest of the C-terminal fragment (residues 251-338) showed that radioactivity was located in a peptide containing a single histidine at position 275. We propose that this conserved residue, in a highly conserved region, is central to the function of the active mixed-valence state.DQ - Departamento de QuímicaRUNMcGinnity, Dermot F.Devreese, BartPrazeres, SusanaVan Beeumen, JozefMourait, IsabelMoura, José J. G.Pettigrew, Graham W.2019-09-09T22:37:13Z1996-06-041996-06-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttps://doi.org/10.1074/jbc.271.19.11126eng0021-9258PURE: 14624705http://www.scopus.com/inward/record.url?scp=17544370121&partnerID=8YFLogxKhttps://doi.org/10.1074/jbc.271.19.11126info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:35:37Zoai:run.unl.pt:10362/80627Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:35:53.040875Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans |
title |
A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans |
spellingShingle |
A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans McGinnity, Dermot F. Biochemistry Molecular Biology Cell Biology |
title_short |
A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans |
title_full |
A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans |
title_fullStr |
A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans |
title_full_unstemmed |
A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans |
title_sort |
A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans |
author |
McGinnity, Dermot F. |
author_facet |
McGinnity, Dermot F. Devreese, Bart Prazeres, Susana Van Beeumen, Jozef Mourait, Isabel Moura, José J. G. Pettigrew, Graham W. |
author_role |
author |
author2 |
Devreese, Bart Prazeres, Susana Van Beeumen, Jozef Mourait, Isabel Moura, José J. G. Pettigrew, Graham W. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
DQ - Departamento de Química RUN |
dc.contributor.author.fl_str_mv |
McGinnity, Dermot F. Devreese, Bart Prazeres, Susana Van Beeumen, Jozef Mourait, Isabel Moura, José J. G. Pettigrew, Graham W. |
dc.subject.por.fl_str_mv |
Biochemistry Molecular Biology Cell Biology |
topic |
Biochemistry Molecular Biology Cell Biology |
description |
Wellcome Trust |
publishDate |
1996 |
dc.date.none.fl_str_mv |
1996-06-04 1996-06-04T00:00:00Z 2019-09-09T22:37:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1074/jbc.271.19.11126 |
url |
https://doi.org/10.1074/jbc.271.19.11126 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0021-9258 PURE: 14624705 http://www.scopus.com/inward/record.url?scp=17544370121&partnerID=8YFLogxK https://doi.org/10.1074/jbc.271.19.11126 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137979102396416 |