Induced autolysis of engineered yeast residue as a means to simplify downstream processing for valorization: a case study
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/42006 |
Resumo: | The objective of this work was to study the efficiency of different autolysis processes, combining different temperatures and pH conditions, when applied to a genetically engineered yeast residue. The determination of the supernatants’ dry weight showed that the autolysis time could be reduced to half, from 4 to 2 h, if the residue pH was increased from 5 to 8 at 50 °C (18.20% for 4 h and 18.70% for 2 h with a higher pH). This result allowed us to select a short autolysis time to proceed with the second part of the experiments. The application of this faster induced autolysis process enabled us to obtain supernatants with higher concentrations of relevant compounds, such as some amino acids and minerals. An increase in leucine (of around 7%), aspartic acid, valine, phenylalanine, isoleucine and serine (approximately 2%) was observed in the autolyzed samples, when compared to the untreated ones. Also, regarding minerals, the autolysis process allowed us to obtain significantly higher amounts of potassium in the treated samples’ supernatants. This work allowed the selection of a fast and low-cost induced autolysis process for synthetic biotechnology-derived spent yeast residue to attain a product rich in high-value compounds, which can be used in commercial applications, for example, as an animal feed additive. |
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Induced autolysis of engineered yeast residue as a means to simplify downstream processing for valorization: a case studySynthetic biotechnologSpent yeast residueNutrient sourceAutolysisPhysicochemical compositionAnimal feedThe objective of this work was to study the efficiency of different autolysis processes, combining different temperatures and pH conditions, when applied to a genetically engineered yeast residue. The determination of the supernatants’ dry weight showed that the autolysis time could be reduced to half, from 4 to 2 h, if the residue pH was increased from 5 to 8 at 50 °C (18.20% for 4 h and 18.70% for 2 h with a higher pH). This result allowed us to select a short autolysis time to proceed with the second part of the experiments. The application of this faster induced autolysis process enabled us to obtain supernatants with higher concentrations of relevant compounds, such as some amino acids and minerals. An increase in leucine (of around 7%), aspartic acid, valine, phenylalanine, isoleucine and serine (approximately 2%) was observed in the autolyzed samples, when compared to the untreated ones. Also, regarding minerals, the autolysis process allowed us to obtain significantly higher amounts of potassium in the treated samples’ supernatants. This work allowed the selection of a fast and low-cost induced autolysis process for synthetic biotechnology-derived spent yeast residue to attain a product rich in high-value compounds, which can be used in commercial applications, for example, as an animal feed additive.Veritati - Repositório Institucional da Universidade Católica PortuguesaFundo, Joana F.Deuchande, TeresaRodrigues, Daniela A.Pimentel, Lígia L.Vidigal, Susana S. M. P.Rodríguez-Alcalá, Luís M.Pintado, Manuela E.Amaro, Ana L.2023-08-02T09:17:02Z2023-07-182023-07-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/42006eng2311-563710.3390/fermentation907067385166425057001038595800001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-22T01:30:39Zoai:repositorio.ucp.pt:10400.14/42006Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:26:18.774634Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Induced autolysis of engineered yeast residue as a means to simplify downstream processing for valorization: a case study |
title |
Induced autolysis of engineered yeast residue as a means to simplify downstream processing for valorization: a case study |
spellingShingle |
Induced autolysis of engineered yeast residue as a means to simplify downstream processing for valorization: a case study Fundo, Joana F. Synthetic biotechnolog Spent yeast residue Nutrient source Autolysis Physicochemical composition Animal feed |
title_short |
Induced autolysis of engineered yeast residue as a means to simplify downstream processing for valorization: a case study |
title_full |
Induced autolysis of engineered yeast residue as a means to simplify downstream processing for valorization: a case study |
title_fullStr |
Induced autolysis of engineered yeast residue as a means to simplify downstream processing for valorization: a case study |
title_full_unstemmed |
Induced autolysis of engineered yeast residue as a means to simplify downstream processing for valorization: a case study |
title_sort |
Induced autolysis of engineered yeast residue as a means to simplify downstream processing for valorization: a case study |
author |
Fundo, Joana F. |
author_facet |
Fundo, Joana F. Deuchande, Teresa Rodrigues, Daniela A. Pimentel, Lígia L. Vidigal, Susana S. M. P. Rodríguez-Alcalá, Luís M. Pintado, Manuela E. Amaro, Ana L. |
author_role |
author |
author2 |
Deuchande, Teresa Rodrigues, Daniela A. Pimentel, Lígia L. Vidigal, Susana S. M. P. Rodríguez-Alcalá, Luís M. Pintado, Manuela E. Amaro, Ana L. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Fundo, Joana F. Deuchande, Teresa Rodrigues, Daniela A. Pimentel, Lígia L. Vidigal, Susana S. M. P. Rodríguez-Alcalá, Luís M. Pintado, Manuela E. Amaro, Ana L. |
dc.subject.por.fl_str_mv |
Synthetic biotechnolog Spent yeast residue Nutrient source Autolysis Physicochemical composition Animal feed |
topic |
Synthetic biotechnolog Spent yeast residue Nutrient source Autolysis Physicochemical composition Animal feed |
description |
The objective of this work was to study the efficiency of different autolysis processes, combining different temperatures and pH conditions, when applied to a genetically engineered yeast residue. The determination of the supernatants’ dry weight showed that the autolysis time could be reduced to half, from 4 to 2 h, if the residue pH was increased from 5 to 8 at 50 °C (18.20% for 4 h and 18.70% for 2 h with a higher pH). This result allowed us to select a short autolysis time to proceed with the second part of the experiments. The application of this faster induced autolysis process enabled us to obtain supernatants with higher concentrations of relevant compounds, such as some amino acids and minerals. An increase in leucine (of around 7%), aspartic acid, valine, phenylalanine, isoleucine and serine (approximately 2%) was observed in the autolyzed samples, when compared to the untreated ones. Also, regarding minerals, the autolysis process allowed us to obtain significantly higher amounts of potassium in the treated samples’ supernatants. This work allowed the selection of a fast and low-cost induced autolysis process for synthetic biotechnology-derived spent yeast residue to attain a product rich in high-value compounds, which can be used in commercial applications, for example, as an animal feed additive. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-08-02T09:17:02Z 2023-07-18 2023-07-18T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/42006 |
url |
http://hdl.handle.net/10400.14/42006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2311-5637 10.3390/fermentation9070673 85166425057 001038595800001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133530727383040 |