Rab GTPase regulation of bacteria and protozoa phagocytosis occurs through the modulation of phagocytic receptor surface expression

Detalhes bibliográficos
Autor(a) principal: Seixas, E.
Data de Publicação: 2018
Outros Autores: Escrevente, C., Seabra, M.C., Barral, D.C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1038/s41598-018-31171-5
Resumo: This work was supported by Fundacao para a Ciencia e a Tecnologia (FCT) I.P., Portugal through grant PTDC/SAU-MII/104622/2008 (to M.C.S. and D.C.B.); the FCT Investigator Program (IF/00501/2014/CP1252/CT0001, to D.C.B.); and FCT post-doctoral fellowships SFRH/BPD/34094/2006 (to E.S.) and SFRH/BPD/78491/2011 (to C.E.).
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spelling Rab GTPase regulation of bacteria and protozoa phagocytosis occurs through the modulation of phagocytic receptor surface expressionSDG 3 - Good Health and Well-beingThis work was supported by Fundacao para a Ciencia e a Tecnologia (FCT) I.P., Portugal through grant PTDC/SAU-MII/104622/2008 (to M.C.S. and D.C.B.); the FCT Investigator Program (IF/00501/2014/CP1252/CT0001, to D.C.B.); and FCT post-doctoral fellowships SFRH/BPD/34094/2006 (to E.S.) and SFRH/BPD/78491/2011 (to C.E.).Phagocytosis of invading microorganisms by professional phagocytic cells has a central role in innate immunity. However, several microorganisms developed strategies to subvert this process. Previously, we reported that bacteria and protozoa modulate differently the expression of Rab GTPases. Moreover, our results suggested that this modulation can contribute to avoid phagocytosis. Here, we investigated the mechanism by which the malaria parasite Plasmodium berghei and the bacterium Escherichia coli subvert phagocytosis through the modulation of Rab14 or Rab9a expression, respectively. We first confirmed that the scavenger receptor CD36 and the Toll-like receptor (TLR) 4 are required for the phagocytosis of P. berghei and E. coli, respectively. Interestingly, we observed that Rab14 silencing leads to an increase in the surface expression of CD36 in macrophages, which can explain the increase in the phagocytosis of P. berghei we reported previously. Similar results were obtained for Rab9a and TLR4, i.e. Rab9a silencing causes an upregulation of TLR4 surface expression in macrophages. Furthermore, we found that the decrease in the internalization of CD36 and TLR4, upon Rab14 or Rab9a silencing, respectively, can explain the increase in the surface levels of these receptors. Thus, our studies provide evidence that the modulation of phagocytosis caused by changes in Rab expression is operated, at least partly through changes in the surface levels of phagocytic receptors. © 2018, The Author(s).NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)RUNSeixas, E.Escrevente, C.Seabra, M.C.Barral, D.C.2018-09-26T22:07:03Z2018-08-292018-08-29T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1038/s41598-018-31171-5eng2045-2322PURE: 5917419https://www.scopus.com/inward/record.uri?eid=2-s2.0-85052627769&doi=10.1038%2fs41598-018-31171-5&partnerID=40&md5=7caf1d753faf557ecc3a662bd2698e26https://doi.org/10.1038/s41598-018-31171-5info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:24:42Zoai:run.unl.pt:10362/47503Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:32:04.561084Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Rab GTPase regulation of bacteria and protozoa phagocytosis occurs through the modulation of phagocytic receptor surface expression
title Rab GTPase regulation of bacteria and protozoa phagocytosis occurs through the modulation of phagocytic receptor surface expression
spellingShingle Rab GTPase regulation of bacteria and protozoa phagocytosis occurs through the modulation of phagocytic receptor surface expression
Seixas, E.
SDG 3 - Good Health and Well-being
title_short Rab GTPase regulation of bacteria and protozoa phagocytosis occurs through the modulation of phagocytic receptor surface expression
title_full Rab GTPase regulation of bacteria and protozoa phagocytosis occurs through the modulation of phagocytic receptor surface expression
title_fullStr Rab GTPase regulation of bacteria and protozoa phagocytosis occurs through the modulation of phagocytic receptor surface expression
title_full_unstemmed Rab GTPase regulation of bacteria and protozoa phagocytosis occurs through the modulation of phagocytic receptor surface expression
title_sort Rab GTPase regulation of bacteria and protozoa phagocytosis occurs through the modulation of phagocytic receptor surface expression
author Seixas, E.
author_facet Seixas, E.
Escrevente, C.
Seabra, M.C.
Barral, D.C.
author_role author
author2 Escrevente, C.
Seabra, M.C.
Barral, D.C.
author2_role author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Centro de Estudos de Doenças Crónicas (CEDOC)
RUN
dc.contributor.author.fl_str_mv Seixas, E.
Escrevente, C.
Seabra, M.C.
Barral, D.C.
dc.subject.por.fl_str_mv SDG 3 - Good Health and Well-being
topic SDG 3 - Good Health and Well-being
description This work was supported by Fundacao para a Ciencia e a Tecnologia (FCT) I.P., Portugal through grant PTDC/SAU-MII/104622/2008 (to M.C.S. and D.C.B.); the FCT Investigator Program (IF/00501/2014/CP1252/CT0001, to D.C.B.); and FCT post-doctoral fellowships SFRH/BPD/34094/2006 (to E.S.) and SFRH/BPD/78491/2011 (to C.E.).
publishDate 2018
dc.date.none.fl_str_mv 2018-09-26T22:07:03Z
2018-08-29
2018-08-29T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1038/s41598-018-31171-5
url https://doi.org/10.1038/s41598-018-31171-5
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2045-2322
PURE: 5917419
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85052627769&doi=10.1038%2fs41598-018-31171-5&partnerID=40&md5=7caf1d753faf557ecc3a662bd2698e26
https://doi.org/10.1038/s41598-018-31171-5
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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