Characterizing the function of two Drosophila Leucine-rich repeat-containing G-protein coupled receptors

Detalhes bibliográficos
Autor(a) principal: Casimiro, Andreia Palos
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.26/7886
Resumo: Dissertação para obtenção do grau de Mestre em Biologia Molecular em Saúde.
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spelling Characterizing the function of two Drosophila Leucine-rich repeat-containing G-protein coupled receptorsDrosophila melanogasterLeucine-reach repeat-containing G-protein-coupled receptorsDrosophila insulin-like peptideDissertação para obtenção do grau de Mestre em Biologia Molecular em Saúde.Relaxin is a hormone structurally similar to insulin, firstly described in 1926, as a substance with a significant influence on the reproductive system. While insulin activates a tyrosine kinase receptor and stimulates signaling pathway that includes phosphoinositide 3-kinase (PI3K) and serine/threorine kinase (AKT), relaxins bind to Leucine-rich repeat-containing G-protein-coupled receptors (LGRs) of the C1 subtype. In Drosophila, two LGRs of subtype C1 exhibit clear structural homology with relaxin receptors, described in mammals. Neverthless, the ligands and the biological functions of these receptors remain unknown not only in Drosophila, but also in invertebrates. Here our objective was to generate genetic tools to study the biological function of these two Type C1 LGRs in Drosophila. With this aim we generated mutant lines for both receptors through classical transposon remobilization techniques. The mutants obtained were characterized as strong loss-of-function deletions, yet no clear phenotype was observed for any of the receptors as regards viability and reproduction. We then tested the hypothesis that either Type C1 LGR could be part of the developmental delay pathway triggered by the Drosophila insuline-like peptide. This peptide is produced and secreted from abnormally growing imaginal discs and delays the onset of metamorphosis by inhibiting the biosynthesis of the major insect molting hormone ecdysone. Strikingly, we found that mutations in either Type C1 LGRs could suppress the insulin-like peptide-depend delay in the onset of metamorphosis to different extents. These results provide the first glimpse into a biological function for invertebrate Type C1 LGR receptors and place them downstream or in parallel to Drosophila insulin-like peptide in this developmental timing control pathway. The resemblance between human and Drosophila core physiological and developmental pathways reinforce that the fly can be a powerful model system to study genes and pathways that are relevant for human development and disease.Gontijo, ÁlissonHerédia, FabianaRepositório ComumCasimiro, Andreia Palos2016-12-30T01:30:15Z2014-09-01T00:00:00Z2014-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.26/7886201541181enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-10-06T14:51:31Zoai:comum.rcaap.pt:10400.26/7886Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:06:13.344094Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Characterizing the function of two Drosophila Leucine-rich repeat-containing G-protein coupled receptors
title Characterizing the function of two Drosophila Leucine-rich repeat-containing G-protein coupled receptors
spellingShingle Characterizing the function of two Drosophila Leucine-rich repeat-containing G-protein coupled receptors
Casimiro, Andreia Palos
Drosophila melanogaster
Leucine-reach repeat-containing G-protein-coupled receptors
Drosophila insulin-like peptide
title_short Characterizing the function of two Drosophila Leucine-rich repeat-containing G-protein coupled receptors
title_full Characterizing the function of two Drosophila Leucine-rich repeat-containing G-protein coupled receptors
title_fullStr Characterizing the function of two Drosophila Leucine-rich repeat-containing G-protein coupled receptors
title_full_unstemmed Characterizing the function of two Drosophila Leucine-rich repeat-containing G-protein coupled receptors
title_sort Characterizing the function of two Drosophila Leucine-rich repeat-containing G-protein coupled receptors
author Casimiro, Andreia Palos
author_facet Casimiro, Andreia Palos
author_role author
dc.contributor.none.fl_str_mv Gontijo, Álisson
Herédia, Fabiana
Repositório Comum
dc.contributor.author.fl_str_mv Casimiro, Andreia Palos
dc.subject.por.fl_str_mv Drosophila melanogaster
Leucine-reach repeat-containing G-protein-coupled receptors
Drosophila insulin-like peptide
topic Drosophila melanogaster
Leucine-reach repeat-containing G-protein-coupled receptors
Drosophila insulin-like peptide
description Dissertação para obtenção do grau de Mestre em Biologia Molecular em Saúde.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-01T00:00:00Z
2014-09-01T00:00:00Z
2016-12-30T01:30:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.26/7886
201541181
url http://hdl.handle.net/10400.26/7886
identifier_str_mv 201541181
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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