Regulation of microtubule-organizing centers during development in Drosophila melanogaster

Detalhes bibliográficos
Autor(a) principal: Cabrita, Jéssica Irina Pisco
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/56935
Resumo: Tese de mestrado, Biologia Molecular e Genética, 2023, Universidade de Lisboa, Faculdade de Ciências
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spelling Regulation of microtubule-organizing centers during development in Drosophila melanogasterWD repeat-containing protein 62 (WDR-62)MTOCs não-centrossomaisDrosophila melanogasterOogéneseTeses de mestrado - 2023Departamento de Biologia VegetalTese de mestrado, Biologia Molecular e Genética, 2023, Universidade de Lisboa, Faculdade de CiênciasThe organization of the microtubule (MT) cytoskeleton is essential for many cellular functions, including cell shape, polarity, migration, and division. The geometry of MT networks critically depends on the localization of sites called microtubule organizing centers (MTOCs), which contain proteins that promote MT nucleation, anchoring, and/or stabilization. The best-studied MTOC is the centrosome, composed of two MT-based centrioles, surrounded by the pericentriolar material (PCM). The PCM is a multicloud of proteins essential for the activity of the centrosome as an MTOC. However, upon mitotic exit or cell differentiation, the activity of the centrosome as an MTOC is generally attenuated and in extreme cases, it can be eliminated. This is often associated with the remodeling of the MT cytoskeleton into cell-type specific MT arrays with the assignment of MTOC function to other places in the cell, named non-centrosomal MTOCs (ncMTOCs). In contrast to centrosomes, we know very little about the composition, structure, and function of ncMTOCs. We set ourselves to investigate how ncMTOCs are assembled and regulated by using the Drosophila germline as a model. I found that upon centrosomal inactivation both in oocytes and follicular cells, the centrosomal MT regulator WDR-62 becomes localized at ncMTOCs. I performed functional studies in the oocytes, which strongly suggest that WDR62 is required for the organization of the MT cytoskeleton. I also found that WDR-62, as well as the conserved ncMTOC component, Shot, are interdependent for their localization at the ncMTOCs. This suggests WDR-62 is a core ncMTOC component. We are currently investigating the role of WDR-62 at ncMTOCs. Wdr62 is the second most common genetic cause of microcephaly in human patients. Therefore, understanding the role of this protein not only in centrosomes but also at ncMTOCs might lead to important insight into its relevance, in normal development but also disease.Marques, Ana Rita PimentaSucena, José Élio da SilvaRepositório da Universidade de LisboaCabrita, Jéssica Irina Pisco202320222026-02-09T00:00:00Z2023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10451/56935enginfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:04:56Zoai:repositorio.ul.pt:10451/56935Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:07:26.108700Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Regulation of microtubule-organizing centers during development in Drosophila melanogaster
title Regulation of microtubule-organizing centers during development in Drosophila melanogaster
spellingShingle Regulation of microtubule-organizing centers during development in Drosophila melanogaster
Cabrita, Jéssica Irina Pisco
WD repeat-containing protein 62 (WDR-62)
MTOCs não-centrossomais
Drosophila melanogaster
Oogénese
Teses de mestrado - 2023
Departamento de Biologia Vegetal
title_short Regulation of microtubule-organizing centers during development in Drosophila melanogaster
title_full Regulation of microtubule-organizing centers during development in Drosophila melanogaster
title_fullStr Regulation of microtubule-organizing centers during development in Drosophila melanogaster
title_full_unstemmed Regulation of microtubule-organizing centers during development in Drosophila melanogaster
title_sort Regulation of microtubule-organizing centers during development in Drosophila melanogaster
author Cabrita, Jéssica Irina Pisco
author_facet Cabrita, Jéssica Irina Pisco
author_role author
dc.contributor.none.fl_str_mv Marques, Ana Rita Pimenta
Sucena, José Élio da Silva
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Cabrita, Jéssica Irina Pisco
dc.subject.por.fl_str_mv WD repeat-containing protein 62 (WDR-62)
MTOCs não-centrossomais
Drosophila melanogaster
Oogénese
Teses de mestrado - 2023
Departamento de Biologia Vegetal
topic WD repeat-containing protein 62 (WDR-62)
MTOCs não-centrossomais
Drosophila melanogaster
Oogénese
Teses de mestrado - 2023
Departamento de Biologia Vegetal
description Tese de mestrado, Biologia Molecular e Genética, 2023, Universidade de Lisboa, Faculdade de Ciências
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023-01-01T00:00:00Z
2026-02-09T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/56935
url http://hdl.handle.net/10451/56935
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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