Exploring metabolic responses of potato tissue induced by electric pulses

Detalhes bibliográficos
Autor(a) principal: Gómez Galindo, Federico
Data de Publicação: 2008
Outros Autores: Wadsö, Lars, Vicente, A. A., Dejmek, Petr
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/8356
Resumo: In this study, we investigated the metabolic responses of potato tissue induced by pulsed electric field (PEF). Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with a single rectangular pulse of 10 μs, 100 μs, or 1 ms. Metabolic responses were monitored using isothermal calorimetry, changes on electrical resistance during the delivery of the pulse, as well as impedance measurements. Our results show that the metabolic response involves oxygen consuming pathways as well as other unidentified events that are shown to be insensitive to metabolic inhibitors such as KCN and sodium azide. The metabolic response is strongly dependent on pulsing conditions and is independent of the total permeabilization achieved by the pulse. Evidence shows that calorimetry is a simple and powerful method for exploring conditions for metabolic stimulation, providing information on metabolic responses that can not be obtained from electrical measurements. This study set the basis for further investigations on defense-related consequences of PEF-induced stress.
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spelling Exploring metabolic responses of potato tissue induced by electric pulsesMetabolic stimulationIsothermal calorimetryImpedanceElectrical resistanceScience & TechnologyIn this study, we investigated the metabolic responses of potato tissue induced by pulsed electric field (PEF). Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with a single rectangular pulse of 10 μs, 100 μs, or 1 ms. Metabolic responses were monitored using isothermal calorimetry, changes on electrical resistance during the delivery of the pulse, as well as impedance measurements. Our results show that the metabolic response involves oxygen consuming pathways as well as other unidentified events that are shown to be insensitive to metabolic inhibitors such as KCN and sodium azide. The metabolic response is strongly dependent on pulsing conditions and is independent of the total permeabilization achieved by the pulse. Evidence shows that calorimetry is a simple and powerful method for exploring conditions for metabolic stimulation, providing information on metabolic responses that can not be obtained from electrical measurements. This study set the basis for further investigations on defense-related consequences of PEF-induced stress.The authors would like to thank Allan G. Rasmusson from the Department of Cell and Organism Biology and Marianne Sommarin from the Department of Plant Biochemistry, Lund University, Sweden for the enlightening discussions. This study was supported by grants from the Portuguese Foundation of Science and Technology and Sparbanksstiftelsen Färs & Frosta, Sweden.SpringerUniversidade do MinhoGómez Galindo, FedericoWadsö, LarsVicente, A. A.Dejmek, Petr2008-122008-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/8356engGómez Galindo, F., Wadsö, L., Vicente, A., & Dejmek, P. (2008, June 3). Exploring Metabolic Responses of Potato Tissue Induced by Electric Pulses. Food Biophysics. Springer Science and Business Media LLC. http://doi.org/10.1007/s11483-008-9086-31557-18581557-186610.1007/s11483-008-9086-3https://link.springer.com/article/10.1007/s11483-008-9086-3info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:02:51Zoai:repositorium.sdum.uminho.pt:1822/8356Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:52:55.439950Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Exploring metabolic responses of potato tissue induced by electric pulses
title Exploring metabolic responses of potato tissue induced by electric pulses
spellingShingle Exploring metabolic responses of potato tissue induced by electric pulses
Gómez Galindo, Federico
Metabolic stimulation
Isothermal calorimetry
Impedance
Electrical resistance
Science & Technology
title_short Exploring metabolic responses of potato tissue induced by electric pulses
title_full Exploring metabolic responses of potato tissue induced by electric pulses
title_fullStr Exploring metabolic responses of potato tissue induced by electric pulses
title_full_unstemmed Exploring metabolic responses of potato tissue induced by electric pulses
title_sort Exploring metabolic responses of potato tissue induced by electric pulses
author Gómez Galindo, Federico
author_facet Gómez Galindo, Federico
Wadsö, Lars
Vicente, A. A.
Dejmek, Petr
author_role author
author2 Wadsö, Lars
Vicente, A. A.
Dejmek, Petr
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gómez Galindo, Federico
Wadsö, Lars
Vicente, A. A.
Dejmek, Petr
dc.subject.por.fl_str_mv Metabolic stimulation
Isothermal calorimetry
Impedance
Electrical resistance
Science & Technology
topic Metabolic stimulation
Isothermal calorimetry
Impedance
Electrical resistance
Science & Technology
description In this study, we investigated the metabolic responses of potato tissue induced by pulsed electric field (PEF). Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with a single rectangular pulse of 10 μs, 100 μs, or 1 ms. Metabolic responses were monitored using isothermal calorimetry, changes on electrical resistance during the delivery of the pulse, as well as impedance measurements. Our results show that the metabolic response involves oxygen consuming pathways as well as other unidentified events that are shown to be insensitive to metabolic inhibitors such as KCN and sodium azide. The metabolic response is strongly dependent on pulsing conditions and is independent of the total permeabilization achieved by the pulse. Evidence shows that calorimetry is a simple and powerful method for exploring conditions for metabolic stimulation, providing information on metabolic responses that can not be obtained from electrical measurements. This study set the basis for further investigations on defense-related consequences of PEF-induced stress.
publishDate 2008
dc.date.none.fl_str_mv 2008-12
2008-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/8356
url https://hdl.handle.net/1822/8356
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gómez Galindo, F., Wadsö, L., Vicente, A., & Dejmek, P. (2008, June 3). Exploring Metabolic Responses of Potato Tissue Induced by Electric Pulses. Food Biophysics. Springer Science and Business Media LLC. http://doi.org/10.1007/s11483-008-9086-3
1557-1858
1557-1866
10.1007/s11483-008-9086-3
https://link.springer.com/article/10.1007/s11483-008-9086-3
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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