Metabolomic evaluation of pulsed electric field-induced stress on potato tissue

Detalhes bibliográficos
Autor(a) principal: Gómez Galindo, Federico
Data de Publicação: 2009
Outros Autores: Dejmek, Petr, Lundgren, Krister, Rasmusson, Allan G., Vicente, A. A., Moritz, Thomas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/9588
Resumo: Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.
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spelling Metabolomic evaluation of pulsed electric field-induced stress on potato tissueCell membrane recoveryElectroporationMetabolic analysisOPLS multivariate data analysisStress responseWoundingScience & TechnologyMetabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.The Royal Physiographic Society in Lund, SwedenPortuguese Foundation of Science (FCT), PortugalDepartment of Cell and Organism Biology, Lund UniversitySpringer VerlagUniversidade do MinhoGómez Galindo, FedericoDejmek, PetrLundgren, KristerRasmusson, Allan G.Vicente, A. A.Moritz, Thomas2009-082009-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/9588eng"Planta". ISSN 0032-0935. 230:3 (Aug. 2009) 469-479.0032-093510.1007/s00425-009-0950-219495789info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:57:25Zoai:repositorium.sdum.uminho.pt:1822/9588Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:47:04.780266Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Metabolomic evaluation of pulsed electric field-induced stress on potato tissue
title Metabolomic evaluation of pulsed electric field-induced stress on potato tissue
spellingShingle Metabolomic evaluation of pulsed electric field-induced stress on potato tissue
Gómez Galindo, Federico
Cell membrane recovery
Electroporation
Metabolic analysis
OPLS multivariate data analysis
Stress response
Wounding
Science & Technology
title_short Metabolomic evaluation of pulsed electric field-induced stress on potato tissue
title_full Metabolomic evaluation of pulsed electric field-induced stress on potato tissue
title_fullStr Metabolomic evaluation of pulsed electric field-induced stress on potato tissue
title_full_unstemmed Metabolomic evaluation of pulsed electric field-induced stress on potato tissue
title_sort Metabolomic evaluation of pulsed electric field-induced stress on potato tissue
author Gómez Galindo, Federico
author_facet Gómez Galindo, Federico
Dejmek, Petr
Lundgren, Krister
Rasmusson, Allan G.
Vicente, A. A.
Moritz, Thomas
author_role author
author2 Dejmek, Petr
Lundgren, Krister
Rasmusson, Allan G.
Vicente, A. A.
Moritz, Thomas
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gómez Galindo, Federico
Dejmek, Petr
Lundgren, Krister
Rasmusson, Allan G.
Vicente, A. A.
Moritz, Thomas
dc.subject.por.fl_str_mv Cell membrane recovery
Electroporation
Metabolic analysis
OPLS multivariate data analysis
Stress response
Wounding
Science & Technology
topic Cell membrane recovery
Electroporation
Metabolic analysis
OPLS multivariate data analysis
Stress response
Wounding
Science & Technology
description Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.
publishDate 2009
dc.date.none.fl_str_mv 2009-08
2009-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/9588
url http://hdl.handle.net/1822/9588
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Planta". ISSN 0032-0935. 230:3 (Aug. 2009) 469-479.
0032-0935
10.1007/s00425-009-0950-2
19495789
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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