Gellan gum-based hydrogels for intervertebral disc tissue engineering applications

Detalhes bibliográficos
Autor(a) principal: Silva-Correia, Joana
Data de Publicação: 2011
Outros Autores: Oliveira, Joaquim M., Caridade, S. G., Oliveira, J. T., Sousa, R. A., Mano, J. F., Reis, R. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/12794
Resumo: Intervertebral disc (IVD) degeneration is a challenging clinical problem that urgently demands viable nucleus pulposus (NP) implant materials. The best suited biomaterial for NP regeneration has yet to be identified, but it is believed that biodegradable hydrogel-based materials are promising candidates. In this work, we have developed ionic- and photo-crosslinked methacrylated gellan gum (GG–MA) hydrogels to be used in acellular and cellular tissue-engineering strategies for the regeneration of IVDs. The physicochemical properties of the developed hydrogels were investigated by Fourier-transform infrared spectroscopy, 1H nuclear magnetic resonance and differential scanning calorimetry. The swelling ability and degradation rate of hydrogels were also analysed in phosphate-buffered saline solution at physiological pH for a period of 30 days. Additionally, the morphology and mechanical properties of the hydrogels were assessed under a scanning electron microscope and dynamic compression, respectively. An in vitro study was carried out to screen possible cytotoxicity of the gellan gum-based hydrogels by culturing rat lung fibroblasts (L929 cells) with hydrogel leachables up to 7 days. The results demonstrated that gellan gum was successfully methacrylated. We observed that the produced GG–MA hydrogels possess improved mechanical properties and lower water uptake ability and degradation rate as compared to gellan gum. This work also revealed that GG–MA hydrogels are non-cytotoxic in vitro, thus being promising biomaterials to be used in IVD tissue-engineering strategies.
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spelling Gellan gum-based hydrogels for intervertebral disc tissue engineering applicationsGellan gumHydrogelsIntervertebral discMethacrylationNucleus pulposusPhoto-crosslinkingTissue engineeringScience & TechnologyIntervertebral disc (IVD) degeneration is a challenging clinical problem that urgently demands viable nucleus pulposus (NP) implant materials. The best suited biomaterial for NP regeneration has yet to be identified, but it is believed that biodegradable hydrogel-based materials are promising candidates. In this work, we have developed ionic- and photo-crosslinked methacrylated gellan gum (GG–MA) hydrogels to be used in acellular and cellular tissue-engineering strategies for the regeneration of IVDs. The physicochemical properties of the developed hydrogels were investigated by Fourier-transform infrared spectroscopy, 1H nuclear magnetic resonance and differential scanning calorimetry. The swelling ability and degradation rate of hydrogels were also analysed in phosphate-buffered saline solution at physiological pH for a period of 30 days. Additionally, the morphology and mechanical properties of the hydrogels were assessed under a scanning electron microscope and dynamic compression, respectively. An in vitro study was carried out to screen possible cytotoxicity of the gellan gum-based hydrogels by culturing rat lung fibroblasts (L929 cells) with hydrogel leachables up to 7 days. The results demonstrated that gellan gum was successfully methacrylated. We observed that the produced GG–MA hydrogels possess improved mechanical properties and lower water uptake ability and degradation rate as compared to gellan gum. This work also revealed that GG–MA hydrogels are non-cytotoxic in vitro, thus being promising biomaterials to be used in IVD tissue-engineering strategies.The authors are grateful for funds provided by the Portuguese Foundation for Science and Technology (FCT) through the POCTI and FEDER programmes, including Project ProteoLight (Grant No. PTDC/FIS/68517/2006). This work was also carried outwith the support of the European Union-funded Collaborative Project Disc Regeneration (Grant No. NMP3-LA-2008-213904).Wiley-BlackwellUniversidade do MinhoSilva-Correia, JoanaOliveira, Joaquim M.Caridade, S. G.Oliveira, J. T.Sousa, R. A.Mano, J. F.Reis, R. L.2011-062011-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/12794eng1932-700510.1002/term.36321604382info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:22:37Zoai:repositorium.sdum.uminho.pt:1822/12794Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:16:08.658898Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Gellan gum-based hydrogels for intervertebral disc tissue engineering applications
title Gellan gum-based hydrogels for intervertebral disc tissue engineering applications
spellingShingle Gellan gum-based hydrogels for intervertebral disc tissue engineering applications
Silva-Correia, Joana
Gellan gum
Hydrogels
Intervertebral disc
Methacrylation
Nucleus pulposus
Photo-crosslinking
Tissue engineering
Science & Technology
title_short Gellan gum-based hydrogels for intervertebral disc tissue engineering applications
title_full Gellan gum-based hydrogels for intervertebral disc tissue engineering applications
title_fullStr Gellan gum-based hydrogels for intervertebral disc tissue engineering applications
title_full_unstemmed Gellan gum-based hydrogels for intervertebral disc tissue engineering applications
title_sort Gellan gum-based hydrogels for intervertebral disc tissue engineering applications
author Silva-Correia, Joana
author_facet Silva-Correia, Joana
Oliveira, Joaquim M.
Caridade, S. G.
Oliveira, J. T.
Sousa, R. A.
Mano, J. F.
Reis, R. L.
author_role author
author2 Oliveira, Joaquim M.
Caridade, S. G.
Oliveira, J. T.
Sousa, R. A.
Mano, J. F.
Reis, R. L.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva-Correia, Joana
Oliveira, Joaquim M.
Caridade, S. G.
Oliveira, J. T.
Sousa, R. A.
Mano, J. F.
Reis, R. L.
dc.subject.por.fl_str_mv Gellan gum
Hydrogels
Intervertebral disc
Methacrylation
Nucleus pulposus
Photo-crosslinking
Tissue engineering
Science & Technology
topic Gellan gum
Hydrogels
Intervertebral disc
Methacrylation
Nucleus pulposus
Photo-crosslinking
Tissue engineering
Science & Technology
description Intervertebral disc (IVD) degeneration is a challenging clinical problem that urgently demands viable nucleus pulposus (NP) implant materials. The best suited biomaterial for NP regeneration has yet to be identified, but it is believed that biodegradable hydrogel-based materials are promising candidates. In this work, we have developed ionic- and photo-crosslinked methacrylated gellan gum (GG–MA) hydrogels to be used in acellular and cellular tissue-engineering strategies for the regeneration of IVDs. The physicochemical properties of the developed hydrogels were investigated by Fourier-transform infrared spectroscopy, 1H nuclear magnetic resonance and differential scanning calorimetry. The swelling ability and degradation rate of hydrogels were also analysed in phosphate-buffered saline solution at physiological pH for a period of 30 days. Additionally, the morphology and mechanical properties of the hydrogels were assessed under a scanning electron microscope and dynamic compression, respectively. An in vitro study was carried out to screen possible cytotoxicity of the gellan gum-based hydrogels by culturing rat lung fibroblasts (L929 cells) with hydrogel leachables up to 7 days. The results demonstrated that gellan gum was successfully methacrylated. We observed that the produced GG–MA hydrogels possess improved mechanical properties and lower water uptake ability and degradation rate as compared to gellan gum. This work also revealed that GG–MA hydrogels are non-cytotoxic in vitro, thus being promising biomaterials to be used in IVD tissue-engineering strategies.
publishDate 2011
dc.date.none.fl_str_mv 2011-06
2011-06-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/12794
url http://hdl.handle.net/1822/12794
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1932-7005
10.1002/term.363
21604382
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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