Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome: results of an international multicentre study.

Detalhes bibliográficos
Autor(a) principal: Amengual, O
Data de Publicação: 2016
Outros Autores: Forastiero R, R, Sugiura-Ogasawara, M, Otomo, K, Oku, K, Favas, C, Alves, JD, Žigon, P, Ambrožič, A, Tomšič, M, Ruiz-Arruza, I, Ruiz-Irastorza, G, Bertolaccini, ML, Norman, GL, Shums, Z, Arai, J, Murashima, A, Tebo, AE, Gerosa, M, Meroni, PL, Rodriguez-Pintó, I, Cervera, R, Swadzba, J, Musial, J, Atsumi, T
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.10/1785
Resumo: OBJECTIVE: A task force of scientists at the International Congress on Antiphospholipid Antibodies recognized that phosphatidylserine-dependent antiprothrombin antibodies (aPS/PT) might contribute to a better identification of antiphospholipid syndrome (APS). Accordingly, initial and replication retrospective, cross-sectional multicentre studies were conducted to ascertain the value of aPS/PT for APS diagnosis. METHODS: In the initial study (eight centres, seven countries), clinical/laboratory data were retrospectively collected. Serum/plasma samples were tested for IgG aPS/PT at Inova Diagnostics (Inova) using two ELISA kits. A replication study (five centres, five countries) was carried out afterwards. RESULTS: In the initial study (n = 247), a moderate agreement between the IgG aPS/PT Inova and MBL ELISA kits was observed (k = 0.598). IgG aPS/PT were more prevalent in APS patients (51%) than in those without (9%), OR 10.8, 95% CI (4.0-29.3), p < 0.0001. Sensitivity, specificity, positive (LR+) and negative (LR-) likelihood ratio of IgG aPS/PT for APS diagnosis were 51%, 91%, 5.9 and 0.5, respectively. In the replication study (n = 214), a moderate/substantial agreement between the IgG aPS/PT results obtained with both ELISA kits was observed (k = 0.630). IgG aPS/PT were more prevalent in APS patients (47%) than in those without (12%), OR 6.4, 95% CI (2.6-16), p < 0.0001. Sensitivity, specificity, LR + and LR- for APS diagnosis were 47%, 88%, 3.9 and 0.6, respectively. CONCLUSIONS: IgG aPS/PT detection is an easily performed laboratory parameter that might contribute to a better and more complete identification of patients with APS.
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spelling Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome: results of an international multicentre study.Systemic lupus erythematosusAntiphospholipid syndromeThrombosisLúpus eritematoso sistémicoOBJECTIVE: A task force of scientists at the International Congress on Antiphospholipid Antibodies recognized that phosphatidylserine-dependent antiprothrombin antibodies (aPS/PT) might contribute to a better identification of antiphospholipid syndrome (APS). Accordingly, initial and replication retrospective, cross-sectional multicentre studies were conducted to ascertain the value of aPS/PT for APS diagnosis. METHODS: In the initial study (eight centres, seven countries), clinical/laboratory data were retrospectively collected. Serum/plasma samples were tested for IgG aPS/PT at Inova Diagnostics (Inova) using two ELISA kits. A replication study (five centres, five countries) was carried out afterwards. RESULTS: In the initial study (n = 247), a moderate agreement between the IgG aPS/PT Inova and MBL ELISA kits was observed (k = 0.598). IgG aPS/PT were more prevalent in APS patients (51%) than in those without (9%), OR 10.8, 95% CI (4.0-29.3), p < 0.0001. Sensitivity, specificity, positive (LR+) and negative (LR-) likelihood ratio of IgG aPS/PT for APS diagnosis were 51%, 91%, 5.9 and 0.5, respectively. In the replication study (n = 214), a moderate/substantial agreement between the IgG aPS/PT results obtained with both ELISA kits was observed (k = 0.630). IgG aPS/PT were more prevalent in APS patients (47%) than in those without (12%), OR 6.4, 95% CI (2.6-16), p < 0.0001. Sensitivity, specificity, LR + and LR- for APS diagnosis were 47%, 88%, 3.9 and 0.6, respectively. CONCLUSIONS: IgG aPS/PT detection is an easily performed laboratory parameter that might contribute to a better and more complete identification of patients with APS.SAGE PublicationsRepositório do Hospital Prof. Doutor Fernando FonsecaAmengual, OForastiero R, RSugiura-Ogasawara, MOtomo, KOku, KFavas, CAlves, JDŽigon, PAmbrožič, ATomšič, MRuiz-Arruza, IRuiz-Irastorza, GBertolaccini, MLNorman, GLShums, ZArai, JMurashima, ATebo, AEGerosa, MMeroni, PLRodriguez-Pintó, ICervera, RSwadzba, JMusial, JAtsumi, T2017-01-10T14:43:58Z2016-01-01T00:00:00Z2016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.10/1785engLupus. 2016 Aug 10. pii: 0961203316660203. [Epub ahead of print]1477-096210.1177/0961203316660203info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-20T15:52:31Zoai:repositorio.hff.min-saude.pt:10400.10/1785Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:52:50.133590Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome: results of an international multicentre study.
title Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome: results of an international multicentre study.
spellingShingle Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome: results of an international multicentre study.
Amengual, O
Systemic lupus erythematosus
Antiphospholipid syndrome
Thrombosis
Lúpus eritematoso sistémico
title_short Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome: results of an international multicentre study.
title_full Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome: results of an international multicentre study.
title_fullStr Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome: results of an international multicentre study.
title_full_unstemmed Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome: results of an international multicentre study.
title_sort Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome: results of an international multicentre study.
author Amengual, O
author_facet Amengual, O
Forastiero R, R
Sugiura-Ogasawara, M
Otomo, K
Oku, K
Favas, C
Alves, JD
Žigon, P
Ambrožič, A
Tomšič, M
Ruiz-Arruza, I
Ruiz-Irastorza, G
Bertolaccini, ML
Norman, GL
Shums, Z
Arai, J
Murashima, A
Tebo, AE
Gerosa, M
Meroni, PL
Rodriguez-Pintó, I
Cervera, R
Swadzba, J
Musial, J
Atsumi, T
author_role author
author2 Forastiero R, R
Sugiura-Ogasawara, M
Otomo, K
Oku, K
Favas, C
Alves, JD
Žigon, P
Ambrožič, A
Tomšič, M
Ruiz-Arruza, I
Ruiz-Irastorza, G
Bertolaccini, ML
Norman, GL
Shums, Z
Arai, J
Murashima, A
Tebo, AE
Gerosa, M
Meroni, PL
Rodriguez-Pintó, I
Cervera, R
Swadzba, J
Musial, J
Atsumi, T
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório do Hospital Prof. Doutor Fernando Fonseca
dc.contributor.author.fl_str_mv Amengual, O
Forastiero R, R
Sugiura-Ogasawara, M
Otomo, K
Oku, K
Favas, C
Alves, JD
Žigon, P
Ambrožič, A
Tomšič, M
Ruiz-Arruza, I
Ruiz-Irastorza, G
Bertolaccini, ML
Norman, GL
Shums, Z
Arai, J
Murashima, A
Tebo, AE
Gerosa, M
Meroni, PL
Rodriguez-Pintó, I
Cervera, R
Swadzba, J
Musial, J
Atsumi, T
dc.subject.por.fl_str_mv Systemic lupus erythematosus
Antiphospholipid syndrome
Thrombosis
Lúpus eritematoso sistémico
topic Systemic lupus erythematosus
Antiphospholipid syndrome
Thrombosis
Lúpus eritematoso sistémico
description OBJECTIVE: A task force of scientists at the International Congress on Antiphospholipid Antibodies recognized that phosphatidylserine-dependent antiprothrombin antibodies (aPS/PT) might contribute to a better identification of antiphospholipid syndrome (APS). Accordingly, initial and replication retrospective, cross-sectional multicentre studies were conducted to ascertain the value of aPS/PT for APS diagnosis. METHODS: In the initial study (eight centres, seven countries), clinical/laboratory data were retrospectively collected. Serum/plasma samples were tested for IgG aPS/PT at Inova Diagnostics (Inova) using two ELISA kits. A replication study (five centres, five countries) was carried out afterwards. RESULTS: In the initial study (n = 247), a moderate agreement between the IgG aPS/PT Inova and MBL ELISA kits was observed (k = 0.598). IgG aPS/PT were more prevalent in APS patients (51%) than in those without (9%), OR 10.8, 95% CI (4.0-29.3), p < 0.0001. Sensitivity, specificity, positive (LR+) and negative (LR-) likelihood ratio of IgG aPS/PT for APS diagnosis were 51%, 91%, 5.9 and 0.5, respectively. In the replication study (n = 214), a moderate/substantial agreement between the IgG aPS/PT results obtained with both ELISA kits was observed (k = 0.630). IgG aPS/PT were more prevalent in APS patients (47%) than in those without (12%), OR 6.4, 95% CI (2.6-16), p < 0.0001. Sensitivity, specificity, LR + and LR- for APS diagnosis were 47%, 88%, 3.9 and 0.6, respectively. CONCLUSIONS: IgG aPS/PT detection is an easily performed laboratory parameter that might contribute to a better and more complete identification of patients with APS.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-01T00:00:00Z
2016-01-01T00:00:00Z
2017-01-10T14:43:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.10/1785
url http://hdl.handle.net/10400.10/1785
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lupus. 2016 Aug 10. pii: 0961203316660203. [Epub ahead of print]
1477-0962
10.1177/0961203316660203
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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