Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota

Detalhes bibliográficos
Autor(a) principal: Cabrita, Alexandra
Data de Publicação: 2021
Outros Autores: David-Palma, Márcia, Brito, Patrícia H., Heitman, Joseph, Coelho, Marco A., Gonçalves, Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/126025
Resumo: SFRH/BPD/79198/2011 01/SAICT/2016 grant 022153 NIH/NIAID R37 award AI39115-23 R01 award AI50113-16.
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spelling Multiple pathways to homothallism in closely related yeast lineages in the basidiomycotaCystofilobasidialesCystofilobasidiumEvolution of sexual reproductionHomothallismMAT lociPhaffiaSexual reproduction in basidiomycetesMicrobiologyVirologySFRH/BPD/79198/2011 01/SAICT/2016 grant 022153 NIH/NIAID R37 award AI39115-23 R01 award AI50113-16.Sexual reproduction in fungi relies on proteins with well-known functions encoded by the mating type (MAT) loci. In the Basidiomycota, MAT loci are often bipar-tite, with the P/R locus encoding pheromone precursors and pheromone receptors and the HD locus encoding heterodimerizing homeodomain transcription factors (Hd1/Hd2). The interplay between different alleles of these genes within a single species usually gen-erates at least two compatible mating types. However, a minority of species are homo-thallic, reproducing sexually without an obligate need for a compatible partner. Here, we examine the organization and function of the MAT loci of Cystofilobasidium capitatum, a species in the order Cystofilobasidiales, which is unusually rich in homothallic species. We determined MAT gene content and organization in C. capitatum and found that it resembles a mating type of the closely related heterothallic species Cystofilobasidium feri-gula. To explain the homothallic sexual reproduction observed in C. capitatum, we exam-ined HD protein interactions in the two Cystofilobasidium species and determined C. capi-tatum MAT gene expression both in a natural setting and upon heterologous expression in Phaffia rhodozyma, a homothallic species belonging to a clade sister to that of Cystofilobasidium. We conclude that the molecular basis for homothallism in C. capitatum appears to be distinct from that previously established for P. rhodozyma. Unlike in the latter species, homothallism in C. capitatum may involve constitutive activation or dis-pensability of the pheromone receptor and the functional replacement of the usual Hd1/ Hd2 heterodimer by an Hd2 homodimer. Overall, our results suggest that homothallism evolved multiple times within the Cystofilobasidiales.DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitRUNCabrita, AlexandraDavid-Palma, MárciaBrito, Patrícia H.Heitman, JosephCoelho, Marco A.Gonçalves, Paula2021-10-13T04:36:22Z2021-01-012021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttp://hdl.handle.net/10362/126025eng2161-2129PURE: 34234677https://doi.org/10.1128/mBio.03130-20info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:06:44Zoai:run.unl.pt:10362/126025Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:45:50.471630Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota
title Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota
spellingShingle Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota
Cabrita, Alexandra
Cystofilobasidiales
Cystofilobasidium
Evolution of sexual reproduction
Homothallism
MAT loci
Phaffia
Sexual reproduction in basidiomycetes
Microbiology
Virology
title_short Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota
title_full Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota
title_fullStr Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota
title_full_unstemmed Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota
title_sort Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota
author Cabrita, Alexandra
author_facet Cabrita, Alexandra
David-Palma, Márcia
Brito, Patrícia H.
Heitman, Joseph
Coelho, Marco A.
Gonçalves, Paula
author_role author
author2 David-Palma, Márcia
Brito, Patrícia H.
Heitman, Joseph
Coelho, Marco A.
Gonçalves, Paula
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv DCV - Departamento de Ciências da Vida
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Cabrita, Alexandra
David-Palma, Márcia
Brito, Patrícia H.
Heitman, Joseph
Coelho, Marco A.
Gonçalves, Paula
dc.subject.por.fl_str_mv Cystofilobasidiales
Cystofilobasidium
Evolution of sexual reproduction
Homothallism
MAT loci
Phaffia
Sexual reproduction in basidiomycetes
Microbiology
Virology
topic Cystofilobasidiales
Cystofilobasidium
Evolution of sexual reproduction
Homothallism
MAT loci
Phaffia
Sexual reproduction in basidiomycetes
Microbiology
Virology
description SFRH/BPD/79198/2011 01/SAICT/2016 grant 022153 NIH/NIAID R37 award AI39115-23 R01 award AI50113-16.
publishDate 2021
dc.date.none.fl_str_mv 2021-10-13T04:36:22Z
2021-01-01
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/126025
url http://hdl.handle.net/10362/126025
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2161-2129
PURE: 34234677
https://doi.org/10.1128/mBio.03130-20
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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