Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/126025 |
Resumo: | SFRH/BPD/79198/2011 01/SAICT/2016 grant 022153 NIH/NIAID R37 award AI39115-23 R01 award AI50113-16. |
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oai:run.unl.pt:10362/126025 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
spelling |
Multiple pathways to homothallism in closely related yeast lineages in the basidiomycotaCystofilobasidialesCystofilobasidiumEvolution of sexual reproductionHomothallismMAT lociPhaffiaSexual reproduction in basidiomycetesMicrobiologyVirologySFRH/BPD/79198/2011 01/SAICT/2016 grant 022153 NIH/NIAID R37 award AI39115-23 R01 award AI50113-16.Sexual reproduction in fungi relies on proteins with well-known functions encoded by the mating type (MAT) loci. In the Basidiomycota, MAT loci are often bipar-tite, with the P/R locus encoding pheromone precursors and pheromone receptors and the HD locus encoding heterodimerizing homeodomain transcription factors (Hd1/Hd2). The interplay between different alleles of these genes within a single species usually gen-erates at least two compatible mating types. However, a minority of species are homo-thallic, reproducing sexually without an obligate need for a compatible partner. Here, we examine the organization and function of the MAT loci of Cystofilobasidium capitatum, a species in the order Cystofilobasidiales, which is unusually rich in homothallic species. We determined MAT gene content and organization in C. capitatum and found that it resembles a mating type of the closely related heterothallic species Cystofilobasidium feri-gula. To explain the homothallic sexual reproduction observed in C. capitatum, we exam-ined HD protein interactions in the two Cystofilobasidium species and determined C. capi-tatum MAT gene expression both in a natural setting and upon heterologous expression in Phaffia rhodozyma, a homothallic species belonging to a clade sister to that of Cystofilobasidium. We conclude that the molecular basis for homothallism in C. capitatum appears to be distinct from that previously established for P. rhodozyma. Unlike in the latter species, homothallism in C. capitatum may involve constitutive activation or dis-pensability of the pheromone receptor and the functional replacement of the usual Hd1/ Hd2 heterodimer by an Hd2 homodimer. Overall, our results suggest that homothallism evolved multiple times within the Cystofilobasidiales.DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitRUNCabrita, AlexandraDavid-Palma, MárciaBrito, Patrícia H.Heitman, JosephCoelho, Marco A.Gonçalves, Paula2021-10-13T04:36:22Z2021-01-012021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttp://hdl.handle.net/10362/126025eng2161-2129PURE: 34234677https://doi.org/10.1128/mBio.03130-20info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:06:44Zoai:run.unl.pt:10362/126025Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:45:50.471630Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota |
title |
Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota |
spellingShingle |
Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota Cabrita, Alexandra Cystofilobasidiales Cystofilobasidium Evolution of sexual reproduction Homothallism MAT loci Phaffia Sexual reproduction in basidiomycetes Microbiology Virology |
title_short |
Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota |
title_full |
Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota |
title_fullStr |
Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota |
title_full_unstemmed |
Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota |
title_sort |
Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota |
author |
Cabrita, Alexandra |
author_facet |
Cabrita, Alexandra David-Palma, Márcia Brito, Patrícia H. Heitman, Joseph Coelho, Marco A. Gonçalves, Paula |
author_role |
author |
author2 |
David-Palma, Márcia Brito, Patrícia H. Heitman, Joseph Coelho, Marco A. Gonçalves, Paula |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
DCV - Departamento de Ciências da Vida UCIBIO - Applied Molecular Biosciences Unit RUN |
dc.contributor.author.fl_str_mv |
Cabrita, Alexandra David-Palma, Márcia Brito, Patrícia H. Heitman, Joseph Coelho, Marco A. Gonçalves, Paula |
dc.subject.por.fl_str_mv |
Cystofilobasidiales Cystofilobasidium Evolution of sexual reproduction Homothallism MAT loci Phaffia Sexual reproduction in basidiomycetes Microbiology Virology |
topic |
Cystofilobasidiales Cystofilobasidium Evolution of sexual reproduction Homothallism MAT loci Phaffia Sexual reproduction in basidiomycetes Microbiology Virology |
description |
SFRH/BPD/79198/2011 01/SAICT/2016 grant 022153 NIH/NIAID R37 award AI39115-23 R01 award AI50113-16. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-13T04:36:22Z 2021-01-01 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/126025 |
url |
http://hdl.handle.net/10362/126025 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2161-2129 PURE: 34234677 https://doi.org/10.1128/mBio.03130-20 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
15 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799138062934999040 |