Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems

Detalhes bibliográficos
Autor(a) principal: Guerreiro, Bruno M.
Data de Publicação: 2021
Outros Autores: Silva, Jorge Carvalho, Lima, João Carlos, Reis, Maria A. M., Freitas, Filomena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/126497
Resumo: UIDP/04378/2020 UIDB/04378/2020 SFRH/BD/144258/2019
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spelling Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systemsAntioxidantCryopreservationFoodFRAPFucoPolHillHydrogen peroxidePolysaccharideRadiationStressUIDP/04378/2020 UIDB/04378/2020 SFRH/BD/144258/2019Reactive oxygen species (ROS) are dangerous sources of macromolecular damage. While most derive from mitochondrial oxidative phosphorylation, their production can be triggered by ex-ogenous stresses, surpassing the extinction capacity of intrinsic antioxidant defense systems of cells. Here, we report the antioxidant activity of FucoPol, a fucose-rich polyanionic polysaccharide produced by Enterobacter A47, containing ca. 17 wt% of negatively charged residues in its struc-ture. Ferric reducing antioxidant power (FRAP) assays coupled to Hill binding kinetics fitting have shown FucoPol can neutralize ferricyanide and Fe3+-TPTZ species at an EC50 of 896 and 602 µg/mL, respectively, with positive binding cooperativity (2.52 ≤ H ≤ 4.85). This reducing power is greater than most polysaccharides reported. Moreover, an optimal 0.25% w/v FucoPol concentration shown previously to be cryo-and photoprotective was also demonstrated to protect Vero cells against H2O2-induced acute exposure not only by attenuating metabolic viability decay, but also by accentuating post-stress proliferation capacity, whilst preserving cell morphology. These results on antioxidant activity provide evidence for the biopolymer’s ability to prevent positive feedback cascades of the radical-producing Fenton reaction. Ultimately, FucoPol provides a biotechnological alternative for implementation in cryopreservation, food supplementation, and photoprotective sunscreen formula design, as all fields benefit from an antioxidant functionality.DQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitLAQV@REQUIMTEDF – Departamento de FísicaCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)RUNGuerreiro, Bruno M.Silva, Jorge CarvalhoLima, João CarlosReis, Maria A. M.Freitas, Filomena2021-10-22T03:43:35Z2021-09-012021-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/126497engPURE: 34359738https://doi.org/10.3390/polym13183020info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:06:56Zoai:run.unl.pt:10362/126497Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:45:54.575290Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems
title Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems
spellingShingle Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems
Guerreiro, Bruno M.
Antioxidant
Cryopreservation
Food
FRAP
FucoPol
Hill
Hydrogen peroxide
Polysaccharide
Radiation
Stress
title_short Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems
title_full Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems
title_fullStr Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems
title_full_unstemmed Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems
title_sort Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems
author Guerreiro, Bruno M.
author_facet Guerreiro, Bruno M.
Silva, Jorge Carvalho
Lima, João Carlos
Reis, Maria A. M.
Freitas, Filomena
author_role author
author2 Silva, Jorge Carvalho
Lima, João Carlos
Reis, Maria A. M.
Freitas, Filomena
author2_role author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
LAQV@REQUIMTE
DF – Departamento de Física
CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
RUN
dc.contributor.author.fl_str_mv Guerreiro, Bruno M.
Silva, Jorge Carvalho
Lima, João Carlos
Reis, Maria A. M.
Freitas, Filomena
dc.subject.por.fl_str_mv Antioxidant
Cryopreservation
Food
FRAP
FucoPol
Hill
Hydrogen peroxide
Polysaccharide
Radiation
Stress
topic Antioxidant
Cryopreservation
Food
FRAP
FucoPol
Hill
Hydrogen peroxide
Polysaccharide
Radiation
Stress
description UIDP/04378/2020 UIDB/04378/2020 SFRH/BD/144258/2019
publishDate 2021
dc.date.none.fl_str_mv 2021-10-22T03:43:35Z
2021-09-01
2021-09-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/126497
url http://hdl.handle.net/10362/126497
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PURE: 34359738
https://doi.org/10.3390/polym13183020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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