Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/126497 |
Resumo: | UIDP/04378/2020 UIDB/04378/2020 SFRH/BD/144258/2019 |
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Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systemsAntioxidantCryopreservationFoodFRAPFucoPolHillHydrogen peroxidePolysaccharideRadiationStressUIDP/04378/2020 UIDB/04378/2020 SFRH/BD/144258/2019Reactive oxygen species (ROS) are dangerous sources of macromolecular damage. While most derive from mitochondrial oxidative phosphorylation, their production can be triggered by ex-ogenous stresses, surpassing the extinction capacity of intrinsic antioxidant defense systems of cells. Here, we report the antioxidant activity of FucoPol, a fucose-rich polyanionic polysaccharide produced by Enterobacter A47, containing ca. 17 wt% of negatively charged residues in its struc-ture. Ferric reducing antioxidant power (FRAP) assays coupled to Hill binding kinetics fitting have shown FucoPol can neutralize ferricyanide and Fe3+-TPTZ species at an EC50 of 896 and 602 µg/mL, respectively, with positive binding cooperativity (2.52 ≤ H ≤ 4.85). This reducing power is greater than most polysaccharides reported. Moreover, an optimal 0.25% w/v FucoPol concentration shown previously to be cryo-and photoprotective was also demonstrated to protect Vero cells against H2O2-induced acute exposure not only by attenuating metabolic viability decay, but also by accentuating post-stress proliferation capacity, whilst preserving cell morphology. These results on antioxidant activity provide evidence for the biopolymer’s ability to prevent positive feedback cascades of the radical-producing Fenton reaction. Ultimately, FucoPol provides a biotechnological alternative for implementation in cryopreservation, food supplementation, and photoprotective sunscreen formula design, as all fields benefit from an antioxidant functionality.DQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitLAQV@REQUIMTEDF – Departamento de FísicaCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)RUNGuerreiro, Bruno M.Silva, Jorge CarvalhoLima, João CarlosReis, Maria A. M.Freitas, Filomena2021-10-22T03:43:35Z2021-09-012021-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/126497engPURE: 34359738https://doi.org/10.3390/polym13183020info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:06:56Zoai:run.unl.pt:10362/126497Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:45:54.575290Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems |
title |
Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems |
spellingShingle |
Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems Guerreiro, Bruno M. Antioxidant Cryopreservation Food FRAP FucoPol Hill Hydrogen peroxide Polysaccharide Radiation Stress |
title_short |
Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems |
title_full |
Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems |
title_fullStr |
Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems |
title_full_unstemmed |
Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems |
title_sort |
Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems |
author |
Guerreiro, Bruno M. |
author_facet |
Guerreiro, Bruno M. Silva, Jorge Carvalho Lima, João Carlos Reis, Maria A. M. Freitas, Filomena |
author_role |
author |
author2 |
Silva, Jorge Carvalho Lima, João Carlos Reis, Maria A. M. Freitas, Filomena |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
DQ - Departamento de Química UCIBIO - Applied Molecular Biosciences Unit LAQV@REQUIMTE DF – Departamento de Física CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) RUN |
dc.contributor.author.fl_str_mv |
Guerreiro, Bruno M. Silva, Jorge Carvalho Lima, João Carlos Reis, Maria A. M. Freitas, Filomena |
dc.subject.por.fl_str_mv |
Antioxidant Cryopreservation Food FRAP FucoPol Hill Hydrogen peroxide Polysaccharide Radiation Stress |
topic |
Antioxidant Cryopreservation Food FRAP FucoPol Hill Hydrogen peroxide Polysaccharide Radiation Stress |
description |
UIDP/04378/2020 UIDB/04378/2020 SFRH/BD/144258/2019 |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-22T03:43:35Z 2021-09-01 2021-09-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/126497 |
url |
http://hdl.handle.net/10362/126497 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PURE: 34359738 https://doi.org/10.3390/polym13183020 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799138063701508096 |