A scalable method to purify reflectins from inclusion bodies
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/154776 |
Resumo: | Associate Laboratory Institute for Health and Bioeconomy – i4HB (LA/P/0140/2020). 2022.11305.BD for C.S. Publisher Copyright: © 2023 The Author(s) |
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RCAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
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A scalable method to purify reflectins from inclusion bodiesBio-based materialsInclusion bodiesPurificationReflectinsSelf-assemblyStructural proteinsAnalytical ChemistryFiltration and SeparationAssociate Laboratory Institute for Health and Bioeconomy – i4HB (LA/P/0140/2020). 2022.11305.BD for C.S. Publisher Copyright: © 2023 The Author(s)Structural proteins are an attractive inspiration for functional biobased materials. In nature, cephalopods skin colour modulation is related to the dynamic self-assembly of a family of structural proteins known as reflectins. To fully reach their potential as engineered bio-based materials, reflectins need to be produced by biotechnological means. One of the challenges is associated with establishing and optimizing reflectin purification processes to achieve the highest yield and productivity. Here, we studied purification strategies for two reflectin sequences from different organisms which were recombinantly expressed in a bacterial host at laboratory scale. Reflectins purification was then assessed by two chromatographic and one non-chromatographic methods. Methods were compared considering final purity and yield, productivity, cost and sustainability. The non-chromatographic method based on inclusion bodies washing presented the most promising results (protein purity > 90% and purification yields up to 88%). Our results contribute to define bioprocessing strategies to address the vision of biodegradable and sustainable protein-based materials.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNLychko, IanaSoares, Cátia LopesDias, Ana Margarida Gonçalves CarvalhoRoque, Ana Cecília Afonso2023-07-03T22:17:41Z2023-06-152023-06-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/154776eng1383-5866PURE: 65212928https://doi.org/10.1016/j.seppur.2023.123736info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:37:13Zoai:run.unl.pt:10362/154776Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:55:45.052953Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A scalable method to purify reflectins from inclusion bodies |
title |
A scalable method to purify reflectins from inclusion bodies |
spellingShingle |
A scalable method to purify reflectins from inclusion bodies Lychko, Iana Bio-based materials Inclusion bodies Purification Reflectins Self-assembly Structural proteins Analytical Chemistry Filtration and Separation |
title_short |
A scalable method to purify reflectins from inclusion bodies |
title_full |
A scalable method to purify reflectins from inclusion bodies |
title_fullStr |
A scalable method to purify reflectins from inclusion bodies |
title_full_unstemmed |
A scalable method to purify reflectins from inclusion bodies |
title_sort |
A scalable method to purify reflectins from inclusion bodies |
author |
Lychko, Iana |
author_facet |
Lychko, Iana Soares, Cátia Lopes Dias, Ana Margarida Gonçalves Carvalho Roque, Ana Cecília Afonso |
author_role |
author |
author2 |
Soares, Cátia Lopes Dias, Ana Margarida Gonçalves Carvalho Roque, Ana Cecília Afonso |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
UCIBIO - Applied Molecular Biosciences Unit DQ - Departamento de Química RUN |
dc.contributor.author.fl_str_mv |
Lychko, Iana Soares, Cátia Lopes Dias, Ana Margarida Gonçalves Carvalho Roque, Ana Cecília Afonso |
dc.subject.por.fl_str_mv |
Bio-based materials Inclusion bodies Purification Reflectins Self-assembly Structural proteins Analytical Chemistry Filtration and Separation |
topic |
Bio-based materials Inclusion bodies Purification Reflectins Self-assembly Structural proteins Analytical Chemistry Filtration and Separation |
description |
Associate Laboratory Institute for Health and Bioeconomy – i4HB (LA/P/0140/2020). 2022.11305.BD for C.S. Publisher Copyright: © 2023 The Author(s) |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-03T22:17:41Z 2023-06-15 2023-06-15T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/154776 |
url |
http://hdl.handle.net/10362/154776 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1383-5866 PURE: 65212928 https://doi.org/10.1016/j.seppur.2023.123736 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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|
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1799138144294010880 |