Staphylococcus epidermidis is largely dependent on iron availability to form biofilms

Detalhes bibliográficos
Autor(a) principal: Oliveira, Fernando E.
Data de Publicação: 2017
Outros Autores: França, Angela, Cerca, Nuno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/47542
Resumo: Supplementary data associated with this article can be found, in the online version, at http://dx.doi.org/10.1016/j.ijmm.2017.08.009.
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spelling Staphylococcus epidermidis is largely dependent on iron availability to form biofilmsStaphylococcus epidermidisbiofilm formationironScience & TechnologySupplementary data associated with this article can be found, in the online version, at http://dx.doi.org/10.1016/j.ijmm.2017.08.009.Staphylococcus epidermidis has long been known as a major bacterial coloniser of the human skin, yet it is also a prominent nosocomial pathogen. Its remarkable ability to assemble structured biofilms has been its major known pathogenic feature to date. Notwithstanding important discoveries that have been accomplished, several questions about S. epidermidis biofilm formation still remain to be elucidated. This study aimed to assess whether iron availability modulates S. epidermidis biofilm formation and, if so, to explore how such modulation occurs. Biofilms of three S. epidermidis strains were grown under iron-enriched/-deficient conditions and several physiologic and transcriptomic changes were assessed. Our data revealed that while physiologic iron levels do not compromise biofilm formation, iron excess or deficiency is detrimental for this process. Conversely, biofilm cells were not affected in the same way when grown planktonically. By studying biofilm cells in detail we found that their viability and cultivability were seriously compromised by iron deficiency. Also, a temporal analysis of biofilm formation revealed that iron excess/deficiency: i) impaired biomass accumulation from 6 h onwards, and ii) induced changes in the biofilm structure, indicating that iron availability plays a pivotal role from an early biofilm development stage. The expression of several putative iron-related genes, namely encoding siderophore biosynthesis/transport-related proteins, was found to be modulated by iron availability, providing a biological validation of their function on S. epidermidis iron metabolism. This study therefore provides evidence that iron plays a pivotal role on S. epidermidis biofilm formation.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/ BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER006684) and the project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124FEDER-027462). FO and AF acknowledges the Portuguese Foundation for Science and Tecnology for the financial support through the individual grants SFRH/BD/101399/2014 and SFRH/BPD/99961/2014. NC is an Investigador FCT.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoOliveira, Fernando E.França, AngelaCerca, Nuno2017-122017-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/47542engOliveira, Fernando E.; França, Angela; Cerca, Nuno, Staphylococcus epidermidis is largely dependent on iron availability to form biofilms. International Journal of Medical Microbiology, 307(8), 552-563, 20171618-06071438-422110.1016/j.ijmm.2017.08.00928939440http://www.journals.elsevier.com/international-journal-of-medical-microbiology/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:24:00Zoai:repositorium.sdum.uminho.pt:1822/47542Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:17:52.408440Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Staphylococcus epidermidis is largely dependent on iron availability to form biofilms
title Staphylococcus epidermidis is largely dependent on iron availability to form biofilms
spellingShingle Staphylococcus epidermidis is largely dependent on iron availability to form biofilms
Oliveira, Fernando E.
Staphylococcus epidermidis
biofilm formation
iron
Science & Technology
title_short Staphylococcus epidermidis is largely dependent on iron availability to form biofilms
title_full Staphylococcus epidermidis is largely dependent on iron availability to form biofilms
title_fullStr Staphylococcus epidermidis is largely dependent on iron availability to form biofilms
title_full_unstemmed Staphylococcus epidermidis is largely dependent on iron availability to form biofilms
title_sort Staphylococcus epidermidis is largely dependent on iron availability to form biofilms
author Oliveira, Fernando E.
author_facet Oliveira, Fernando E.
França, Angela
Cerca, Nuno
author_role author
author2 França, Angela
Cerca, Nuno
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Oliveira, Fernando E.
França, Angela
Cerca, Nuno
dc.subject.por.fl_str_mv Staphylococcus epidermidis
biofilm formation
iron
Science & Technology
topic Staphylococcus epidermidis
biofilm formation
iron
Science & Technology
description Supplementary data associated with this article can be found, in the online version, at http://dx.doi.org/10.1016/j.ijmm.2017.08.009.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
2017-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/47542
url http://hdl.handle.net/1822/47542
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Oliveira, Fernando E.; França, Angela; Cerca, Nuno, Staphylococcus epidermidis is largely dependent on iron availability to form biofilms. International Journal of Medical Microbiology, 307(8), 552-563, 2017
1618-0607
1438-4221
10.1016/j.ijmm.2017.08.009
28939440
http://www.journals.elsevier.com/international-journal-of-medical-microbiology/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
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