Deciphering the role of cartilage protein 1 in human dermal fibroblasts: a transcriptomic approach

• Autor(a) principal: Letsiou, Sophia
• Data de Publicação: 2021
• Outros Autores: Manchado, Manuel, Zografaki, Mariela, Marka, Sofia, L, Anjos, Skliros, Dimitrios, Martínez-Blanch, Juan F., Flemetakis, E., Power, Deborah
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.1/16866
• topic: hCRTAC1; Human fibroblasts; Transcriptomics; ECM

Cartilage acidic protein 1A (hCRTAC1-A) is an extracellular matrix protein (ECM) of human hard and soft tissue that is associated with matrix disorders. The central role of fibroblasts in tissue integrity and ECM health made primary human dermal fibroblasts (NHDF) the model for the present study, which aimed to provide new insight into the molecular function of hCRTAC1-A. Specifically, we explored the differential expression patterns of specific genes associated with the presence of hCRTAC1-A by RNA-seq and RT-qPCR analysis. Functional enrichment analysis demonstrated, for the very first time, that hCRTAC1-A is involved in extracellular matrix organization and development, through its regulatory effect on asporin, decorin, and complement activity, in cell proliferation, regeneration, wound healing, and collagen degradation. This work provides a better understanding of putative hCRTAC1-A actions in human fibroblasts and a fundamental insight into its function in tissue biology.