Application of an impedimetric technique for the detection of lytic infection of salmonella spp. by specific phages
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/3258 |
Resumo: | This study was performed to evaluate the adaption of the impedimetric method to detect the lytic infection by Salmonella-specific bacteriophages and to provide a higher selectivity to this rapid method in detecting Salmonella spp. by using specific agents. Three bacteriophages and twelve strains of Salmonella spp. were tested. Each of the twelve strains was used separately to inoculate TSB together with each one of the phages. The inoculumconcentration was between 106 and 107 cfu/mL, at a cell: phage ratio of 1 : 100. From the sample analysis, based on conductance (G)measurements (37◦C), the infection could be detected, by observation of both detection-time delay and distinct curve trends. The main conclusions were that kinetic detection by impedance microbiology with phage typing constitutes a method of determining whether a test microorganism is sensitive to the bacteriophage and a method to evaluate whether a lytic bacteriophage is present in a sample, by affecting bacterial growth rate/metabolic change |
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Application of an impedimetric technique for the detection of lytic infection of salmonella spp. by specific phagesThis study was performed to evaluate the adaption of the impedimetric method to detect the lytic infection by Salmonella-specific bacteriophages and to provide a higher selectivity to this rapid method in detecting Salmonella spp. by using specific agents. Three bacteriophages and twelve strains of Salmonella spp. were tested. Each of the twelve strains was used separately to inoculate TSB together with each one of the phages. The inoculumconcentration was between 106 and 107 cfu/mL, at a cell: phage ratio of 1 : 100. From the sample analysis, based on conductance (G)measurements (37◦C), the infection could be detected, by observation of both detection-time delay and distinct curve trends. The main conclusions were that kinetic detection by impedance microbiology with phage typing constitutes a method of determining whether a test microorganism is sensitive to the bacteriophage and a method to evaluate whether a lytic bacteriophage is present in a sample, by affecting bacterial growth rate/metabolic changeHindawi Publishing CorporationVeritati - Repositório Institucional da Universidade Católica PortuguesaAmorim, Lara R. P.Silva, Joana G. L.Gibbs, Paul A.Teixeira, Paula C.2010-11-02T14:27:30Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/3258engAMORIM, Lara R. P ...[et al.] - Application of an impedimetric technique for the detection of lytic infection of salmonella spp. by specific phages. International Journal of Microbiology. ISSN 1687-918X. (2009), p. 1-610.1155/2009/259456info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:09:55Zoai:repositorio.ucp.pt:10400.14/3258Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:05:21.910345Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Application of an impedimetric technique for the detection of lytic infection of salmonella spp. by specific phages |
title |
Application of an impedimetric technique for the detection of lytic infection of salmonella spp. by specific phages |
spellingShingle |
Application of an impedimetric technique for the detection of lytic infection of salmonella spp. by specific phages Amorim, Lara R. P. |
title_short |
Application of an impedimetric technique for the detection of lytic infection of salmonella spp. by specific phages |
title_full |
Application of an impedimetric technique for the detection of lytic infection of salmonella spp. by specific phages |
title_fullStr |
Application of an impedimetric technique for the detection of lytic infection of salmonella spp. by specific phages |
title_full_unstemmed |
Application of an impedimetric technique for the detection of lytic infection of salmonella spp. by specific phages |
title_sort |
Application of an impedimetric technique for the detection of lytic infection of salmonella spp. by specific phages |
author |
Amorim, Lara R. P. |
author_facet |
Amorim, Lara R. P. Silva, Joana G. L. Gibbs, Paul A. Teixeira, Paula C. |
author_role |
author |
author2 |
Silva, Joana G. L. Gibbs, Paul A. Teixeira, Paula C. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Amorim, Lara R. P. Silva, Joana G. L. Gibbs, Paul A. Teixeira, Paula C. |
description |
This study was performed to evaluate the adaption of the impedimetric method to detect the lytic infection by Salmonella-specific bacteriophages and to provide a higher selectivity to this rapid method in detecting Salmonella spp. by using specific agents. Three bacteriophages and twelve strains of Salmonella spp. were tested. Each of the twelve strains was used separately to inoculate TSB together with each one of the phages. The inoculumconcentration was between 106 and 107 cfu/mL, at a cell: phage ratio of 1 : 100. From the sample analysis, based on conductance (G)measurements (37◦C), the infection could be detected, by observation of both detection-time delay and distinct curve trends. The main conclusions were that kinetic detection by impedance microbiology with phage typing constitutes a method of determining whether a test microorganism is sensitive to the bacteriophage and a method to evaluate whether a lytic bacteriophage is present in a sample, by affecting bacterial growth rate/metabolic change |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2009-01-01T00:00:00Z 2010-11-02T14:27:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/3258 |
url |
http://hdl.handle.net/10400.14/3258 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
AMORIM, Lara R. P ...[et al.] - Application of an impedimetric technique for the detection of lytic infection of salmonella spp. by specific phages. International Journal of Microbiology. ISSN 1687-918X. (2009), p. 1-6 10.1155/2009/259456 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131717895716864 |