Immunohistochemical analysis of collagen content and types in the rectus abdominis muscle of cadavers of different ages

Detalhes bibliográficos
Autor(a) principal: Calvi, Eliziane Nitz de Carvalho [UNIFESP]
Data de Publicação: 2011
Outros Autores: Nahas, Fabio Xerfan [UNIFESP], Barbosa, Marcus Vinicius [UNIFESP], Ihara, Silvia Saiuli Miki [UNIFESP], Calil, José Augusto, Ferreira, Lydia Masako [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0102-86502011000800002
http://repositorio.unifesp.br/handle/11600/6135
Resumo: PURPOSE: To assess the collagen content and types in the rectus abdominis muscle of cadavers of different ages. METHODS: Forty fresh adult male cadavers, at room temperature, were obtained from the Institute of Legal Medicine of Franca and dissected within 24 hours of death. The cadavers were divided into two groups: Group A (n=20), 18 to 30 years of age, and Group B (n=20), 31 to 60 years of age. Bilateral incisions were made in the middle portion of anterior rectus sheath 3 cm superiorly and 2 cm inferiorly to the umbilicus and four fragments of the rectus abdominis muscle were dissected. The samples were fixed in 10% buffered formalin and sent for immunohistochemical analysis to determine collagen content and types. RESULTS: Immunohistochemical results revealed higher amounts of type I and type III collagen in Group A. However, no difference in the amount of type IV collagen was found between the groups. CONCLUSION: The amount of type I and type III collagen was higher in group A.
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spelling Immunohistochemical analysis of collagen content and types in the rectus abdominis muscle of cadavers of different agesImunohistoquímica do colágeno no músculo reto do abdome de cadáveres de diferentes grupos etáriosCollagenRectus AbdominisAge GroupsCadaverColágenoReto do AbdomeGrupos EtáriosCadáverPURPOSE: To assess the collagen content and types in the rectus abdominis muscle of cadavers of different ages. METHODS: Forty fresh adult male cadavers, at room temperature, were obtained from the Institute of Legal Medicine of Franca and dissected within 24 hours of death. The cadavers were divided into two groups: Group A (n=20), 18 to 30 years of age, and Group B (n=20), 31 to 60 years of age. Bilateral incisions were made in the middle portion of anterior rectus sheath 3 cm superiorly and 2 cm inferiorly to the umbilicus and four fragments of the rectus abdominis muscle were dissected. The samples were fixed in 10% buffered formalin and sent for immunohistochemical analysis to determine collagen content and types. RESULTS: Immunohistochemical results revealed higher amounts of type I and type III collagen in Group A. However, no difference in the amount of type IV collagen was found between the groups. CONCLUSION: The amount of type I and type III collagen was higher in group A.OBJETIVO: Avaliar o colágeno no músculo reto do abdome em cadáveres de diferentes faixas etárias. MÉTODOS: Foram dissecados 40 cadáveres adultos masculinos, não fixados, com tempo de óbito de até 24 horas, em temperatura ambiente, provenientes do Instituto Médico-Legal de Franca (SP - Brasil). Os cadáveres foram distribuídos em dois grupos: GRUPO A (n=20) - 18 a 30 anos e GRUPO B (n=20) -31 a 60 anos. Realizou-se incisão na porção mediana da lâmina anterior da bainha do músculo reto a 3 cm superiormente e 2 cm inferiormente ao umbigo em ambos os lados, sendo retirados quatro fragmentos de músculo reto do abdome. Esse material foi conservado em formalina tamponado a 10% e enviado para imuno-histoquímica para determinação do tipo de colágeno. RESULTADOS: Na Imunihistoquímica os colágenos I e III foram estatisticamente maiores no grupo A, porém não houve diferença entre os grupos em relação ao colágeno IV. CONCLUSÃO: A quantidade de colágeno tipo I e III foi maior no grupo A.UNINOVE Department of Physical TherapyUNIFESPUniversidade Federal de São Paulo (UNIFESP) Department of PathologyUNIFESP Head of the Department of SurgeryUNIFESP, Department of PathologyUNIFESP, Head of the Department of SurgerySciELOSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUNINOVE Department of Physical TherapyUniversidade Federal de São Paulo (UNIFESP)Calvi, Eliziane Nitz de Carvalho [UNIFESP]Nahas, Fabio Xerfan [UNIFESP]Barbosa, Marcus Vinicius [UNIFESP]Ihara, Silvia Saiuli Miki [UNIFESP]Calil, José AugustoFerreira, Lydia Masako [UNIFESP]2015-06-14T13:42:43Z2015-06-14T13:42:43Z2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion3-7application/pdfhttp://dx.doi.org/10.1590/S0102-86502011000800002Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 26, p. 3-7, 2011.10.1590/S0102-86502011000800002S0102-86502011000800002.pdf0102-8650S0102-86502011000800002http://repositorio.unifesp.br/handle/11600/6135WOS:000297572000002engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T01:44:33Zoai:repositorio.unifesp.br/:11600/6135Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T01:44:33Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Immunohistochemical analysis of collagen content and types in the rectus abdominis muscle of cadavers of different ages
Imunohistoquímica do colágeno no músculo reto do abdome de cadáveres de diferentes grupos etários
title Immunohistochemical analysis of collagen content and types in the rectus abdominis muscle of cadavers of different ages
spellingShingle Immunohistochemical analysis of collagen content and types in the rectus abdominis muscle of cadavers of different ages
Calvi, Eliziane Nitz de Carvalho [UNIFESP]
Collagen
Rectus Abdominis
Age Groups
Cadaver
Colágeno
Reto do Abdome
Grupos Etários
Cadáver
title_short Immunohistochemical analysis of collagen content and types in the rectus abdominis muscle of cadavers of different ages
title_full Immunohistochemical analysis of collagen content and types in the rectus abdominis muscle of cadavers of different ages
title_fullStr Immunohistochemical analysis of collagen content and types in the rectus abdominis muscle of cadavers of different ages
title_full_unstemmed Immunohistochemical analysis of collagen content and types in the rectus abdominis muscle of cadavers of different ages
title_sort Immunohistochemical analysis of collagen content and types in the rectus abdominis muscle of cadavers of different ages
author Calvi, Eliziane Nitz de Carvalho [UNIFESP]
author_facet Calvi, Eliziane Nitz de Carvalho [UNIFESP]
Nahas, Fabio Xerfan [UNIFESP]
Barbosa, Marcus Vinicius [UNIFESP]
Ihara, Silvia Saiuli Miki [UNIFESP]
Calil, José Augusto
Ferreira, Lydia Masako [UNIFESP]
author_role author
author2 Nahas, Fabio Xerfan [UNIFESP]
Barbosa, Marcus Vinicius [UNIFESP]
Ihara, Silvia Saiuli Miki [UNIFESP]
Calil, José Augusto
Ferreira, Lydia Masako [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv UNINOVE Department of Physical Therapy
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Calvi, Eliziane Nitz de Carvalho [UNIFESP]
Nahas, Fabio Xerfan [UNIFESP]
Barbosa, Marcus Vinicius [UNIFESP]
Ihara, Silvia Saiuli Miki [UNIFESP]
Calil, José Augusto
Ferreira, Lydia Masako [UNIFESP]
dc.subject.por.fl_str_mv Collagen
Rectus Abdominis
Age Groups
Cadaver
Colágeno
Reto do Abdome
Grupos Etários
Cadáver
topic Collagen
Rectus Abdominis
Age Groups
Cadaver
Colágeno
Reto do Abdome
Grupos Etários
Cadáver
description PURPOSE: To assess the collagen content and types in the rectus abdominis muscle of cadavers of different ages. METHODS: Forty fresh adult male cadavers, at room temperature, were obtained from the Institute of Legal Medicine of Franca and dissected within 24 hours of death. The cadavers were divided into two groups: Group A (n=20), 18 to 30 years of age, and Group B (n=20), 31 to 60 years of age. Bilateral incisions were made in the middle portion of anterior rectus sheath 3 cm superiorly and 2 cm inferiorly to the umbilicus and four fragments of the rectus abdominis muscle were dissected. The samples were fixed in 10% buffered formalin and sent for immunohistochemical analysis to determine collagen content and types. RESULTS: Immunohistochemical results revealed higher amounts of type I and type III collagen in Group A. However, no difference in the amount of type IV collagen was found between the groups. CONCLUSION: The amount of type I and type III collagen was higher in group A.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
2015-06-14T13:42:43Z
2015-06-14T13:42:43Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502011000800002
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 26, p. 3-7, 2011.
10.1590/S0102-86502011000800002
S0102-86502011000800002.pdf
0102-8650
S0102-86502011000800002
http://repositorio.unifesp.br/handle/11600/6135
WOS:000297572000002
url http://dx.doi.org/10.1590/S0102-86502011000800002
http://repositorio.unifesp.br/handle/11600/6135
identifier_str_mv Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 26, p. 3-7, 2011.
10.1590/S0102-86502011000800002
S0102-86502011000800002.pdf
0102-8650
S0102-86502011000800002
WOS:000297572000002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirurgica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 3-7
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268306771673088

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