An experimental model for the study of collagen fibers in skeletal muscle
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1590/S0102-86502012001000003 http://repositorio.unifesp.br/handle/11600/7321 |
Resumo: | PURPOSE: To examine histological and histomorphometric techniques for measuring collagen in skeletal muscle. METHODS: The following staining methods were used in the study: hematoxylin and eosin, Masson's trichrome, reticulin, and picrosirius red, and immunostaining for collagen types I, II, III, IV, and V. Histomorphometric measurements were performed using Corel PhotoPaint and UTHSCSA Image Tool 3.0 software. RESULTS: Both the Masson's trichrome and picrosirius red staining provided the best visualization for the measurement of collagen content. CONCLUSION: This methodology is important for the identification and quantification of the different types of collagen in muscles and can be used in the investigation of the qualitative and quantitative influence of collagen on physical activities, aging, and diseases. |
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An experimental model for the study of collagen fibers in skeletal muscleModelo experimental para o estudo de fibras de colágeno no músculo esqueléticoCollagenMuscle, SkeletalHistologyRectus AbdominisCadaverColágenoMúsculo EsqueléticoHistologiaReto do AbdomeCadáverPURPOSE: To examine histological and histomorphometric techniques for measuring collagen in skeletal muscle. METHODS: The following staining methods were used in the study: hematoxylin and eosin, Masson's trichrome, reticulin, and picrosirius red, and immunostaining for collagen types I, II, III, IV, and V. Histomorphometric measurements were performed using Corel PhotoPaint and UTHSCSA Image Tool 3.0 software. RESULTS: Both the Masson's trichrome and picrosirius red staining provided the best visualization for the measurement of collagen content. CONCLUSION: This methodology is important for the identification and quantification of the different types of collagen in muscles and can be used in the investigation of the qualitative and quantitative influence of collagen on physical activities, aging, and diseases.OBJETIVO: Examinar os procedimentos empregados na quantificação do colágeno por métodos histológicos e histomorfométricos. MÉTODOS: Foram utilizadas colorações histológicas por HE, Tricrômico de Masson, Reticulina, Picrossírius e reação imuno-histoquímica para colágeno I, II, III, IV e V. A quantificação histomorfométrica foi realizada utilizando-se os programas Corel PhotoPaint e Image Tool versão 3.0. RESULTADOS: Os métodos histológicos de Masson e Picrossírius apresentaram uma maior facilidade na quantificação do colágeno. CONCLUSÃO: Este modelo é importante para que possa ser identificado e quantificado os diferentes tipos de colágenos nos músculos e relacionar com a atividade física, envelhecimento e doenças.UNIFESPUNIFESPSciELOSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade Federal de São Paulo (UNIFESP)Calvi, Eliziane Nitz de Carvalho [UNIFESP]Nahas, Fabio Xerfan [UNIFESP]Barbosa, Marcus Vinicius [UNIFESP]Calil, José AugustoIhara, Silvia Saiuli Miki [UNIFESP]Silva, Marcelo de SouzaFranco, Marcello Fabiano de [UNIFESP]Ferreira, Lydia Masako [UNIFESP]2015-06-14T13:44:58Z2015-06-14T13:44:58Z2012-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion681-686application/pdfhttp://dx.doi.org/10.1590/S0102-86502012001000003Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 27, n. 10, p. 681-686, 2012.10.1590/S0102-86502012001000003S0102-86502012001000003.pdf0102-8650S0102-86502012001000003http://repositorio.unifesp.br/handle/11600/7321WOS:000309425300003engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T20:28:49Zoai:repositorio.unifesp.br/:11600/7321Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T20:28:49Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
An experimental model for the study of collagen fibers in skeletal muscle Modelo experimental para o estudo de fibras de colágeno no músculo esquelético |
| title |
An experimental model for the study of collagen fibers in skeletal muscle |
| spellingShingle |
An experimental model for the study of collagen fibers in skeletal muscle Calvi, Eliziane Nitz de Carvalho [UNIFESP] Collagen Muscle, Skeletal Histology Rectus Abdominis Cadaver Colágeno Músculo Esquelético Histologia Reto do Abdome Cadáver |
| title_short |
An experimental model for the study of collagen fibers in skeletal muscle |
| title_full |
An experimental model for the study of collagen fibers in skeletal muscle |
| title_fullStr |
An experimental model for the study of collagen fibers in skeletal muscle |
| title_full_unstemmed |
An experimental model for the study of collagen fibers in skeletal muscle |
| title_sort |
An experimental model for the study of collagen fibers in skeletal muscle |
| author |
Calvi, Eliziane Nitz de Carvalho [UNIFESP] |
| author_facet |
Calvi, Eliziane Nitz de Carvalho [UNIFESP] Nahas, Fabio Xerfan [UNIFESP] Barbosa, Marcus Vinicius [UNIFESP] Calil, José Augusto Ihara, Silvia Saiuli Miki [UNIFESP] Silva, Marcelo de Souza Franco, Marcello Fabiano de [UNIFESP] Ferreira, Lydia Masako [UNIFESP] |
| author_role |
author |
| author2 |
Nahas, Fabio Xerfan [UNIFESP] Barbosa, Marcus Vinicius [UNIFESP] Calil, José Augusto Ihara, Silvia Saiuli Miki [UNIFESP] Silva, Marcelo de Souza Franco, Marcello Fabiano de [UNIFESP] Ferreira, Lydia Masako [UNIFESP] |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
| dc.contributor.author.fl_str_mv |
Calvi, Eliziane Nitz de Carvalho [UNIFESP] Nahas, Fabio Xerfan [UNIFESP] Barbosa, Marcus Vinicius [UNIFESP] Calil, José Augusto Ihara, Silvia Saiuli Miki [UNIFESP] Silva, Marcelo de Souza Franco, Marcello Fabiano de [UNIFESP] Ferreira, Lydia Masako [UNIFESP] |
| dc.subject.por.fl_str_mv |
Collagen Muscle, Skeletal Histology Rectus Abdominis Cadaver Colágeno Músculo Esquelético Histologia Reto do Abdome Cadáver |
| topic |
Collagen Muscle, Skeletal Histology Rectus Abdominis Cadaver Colágeno Músculo Esquelético Histologia Reto do Abdome Cadáver |
| description |
PURPOSE: To examine histological and histomorphometric techniques for measuring collagen in skeletal muscle. METHODS: The following staining methods were used in the study: hematoxylin and eosin, Masson's trichrome, reticulin, and picrosirius red, and immunostaining for collagen types I, II, III, IV, and V. Histomorphometric measurements were performed using Corel PhotoPaint and UTHSCSA Image Tool 3.0 software. RESULTS: Both the Masson's trichrome and picrosirius red staining provided the best visualization for the measurement of collagen content. CONCLUSION: This methodology is important for the identification and quantification of the different types of collagen in muscles and can be used in the investigation of the qualitative and quantitative influence of collagen on physical activities, aging, and diseases. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012-10-01 2015-06-14T13:44:58Z 2015-06-14T13:44:58Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-86502012001000003 Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 27, n. 10, p. 681-686, 2012. 10.1590/S0102-86502012001000003 S0102-86502012001000003.pdf 0102-8650 S0102-86502012001000003 http://repositorio.unifesp.br/handle/11600/7321 WOS:000309425300003 |
| url |
http://dx.doi.org/10.1590/S0102-86502012001000003 http://repositorio.unifesp.br/handle/11600/7321 |
| identifier_str_mv |
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 27, n. 10, p. 681-686, 2012. 10.1590/S0102-86502012001000003 S0102-86502012001000003.pdf 0102-8650 S0102-86502012001000003 WOS:000309425300003 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Acta Cirurgica Brasileira |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
681-686 application/pdf |
| dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| instname_str |
Universidade Federal de São Paulo (UNIFESP) |
| instacron_str |
UNIFESP |
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UNIFESP |
| reponame_str |
Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
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biblioteca.csp@unifesp.br |
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1814268400698916864 |