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In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells

Detalhes bibliográficos
Autor(a) principal: Pereira-Junior,Oduvaldo Câmara Marques
Data de Publicação: 2013
Outros Autores: Rahal,Sheila Canevese, Lima-Neto,João Ferreira, Landim-Alvarenga,Fernanda da Cruz, Monteiro,Frederico Ozanan Barros
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000500006
Resumo: PURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker. RESULTS: The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane. CONCLUSION: All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy.
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spelling In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cellsHydroxyapatitesBone MatrixCastor OilPolyurethanesStem CellsPURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker. RESULTS: The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane. CONCLUSION: All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2013-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000500006Acta Cirúrgica Brasileira v.28 n.5 2013reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502013000500006info:eu-repo/semantics/openAccessPereira-Junior,Oduvaldo Câmara MarquesRahal,Sheila CaneveseLima-Neto,João FerreiraLandim-Alvarenga,Fernanda da CruzMonteiro,Frederico Ozanan Barroseng2013-05-20T00:00:00Zoai:scielo:S0102-86502013000500006Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2013-05-20T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
title In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
spellingShingle In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
Pereira-Junior,Oduvaldo Câmara Marques
Hydroxyapatites
Bone Matrix
Castor Oil
Polyurethanes
Stem Cells
title_short In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
title_full In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
title_fullStr In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
title_full_unstemmed In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
title_sort In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
author Pereira-Junior,Oduvaldo Câmara Marques
author_facet Pereira-Junior,Oduvaldo Câmara Marques
Rahal,Sheila Canevese
Lima-Neto,João Ferreira
Landim-Alvarenga,Fernanda da Cruz
Monteiro,Frederico Ozanan Barros
author_role author
author2 Rahal,Sheila Canevese
Lima-Neto,João Ferreira
Landim-Alvarenga,Fernanda da Cruz
Monteiro,Frederico Ozanan Barros
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Pereira-Junior,Oduvaldo Câmara Marques
Rahal,Sheila Canevese
Lima-Neto,João Ferreira
Landim-Alvarenga,Fernanda da Cruz
Monteiro,Frederico Ozanan Barros
dc.subject.por.fl_str_mv Hydroxyapatites
Bone Matrix
Castor Oil
Polyurethanes
Stem Cells
topic Hydroxyapatites
Bone Matrix
Castor Oil
Polyurethanes
Stem Cells
description PURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker. RESULTS: The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane. CONCLUSION: All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy.
publishDate 2013
dc.date.none.fl_str_mv 2013-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000500006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000500006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502013000500006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.28 n.5 2013
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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