In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000500006 |
Resumo: | PURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker. RESULTS: The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane. CONCLUSION: All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy. |
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Acta Cirúrgica Brasileira (Online) |
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In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cellsHydroxyapatitesBone MatrixCastor OilPolyurethanesStem CellsPURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker. RESULTS: The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane. CONCLUSION: All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2013-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000500006Acta Cirúrgica Brasileira v.28 n.5 2013reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502013000500006info:eu-repo/semantics/openAccessPereira-Junior,Oduvaldo Câmara MarquesRahal,Sheila CaneveseLima-Neto,João FerreiraLandim-Alvarenga,Fernanda da CruzMonteiro,Frederico Ozanan Barroseng2013-05-20T00:00:00Zoai:scielo:S0102-86502013000500006Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2013-05-20T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells |
title |
In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells |
spellingShingle |
In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells Pereira-Junior,Oduvaldo Câmara Marques Hydroxyapatites Bone Matrix Castor Oil Polyurethanes Stem Cells |
title_short |
In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells |
title_full |
In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells |
title_fullStr |
In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells |
title_full_unstemmed |
In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells |
title_sort |
In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells |
author |
Pereira-Junior,Oduvaldo Câmara Marques |
author_facet |
Pereira-Junior,Oduvaldo Câmara Marques Rahal,Sheila Canevese Lima-Neto,João Ferreira Landim-Alvarenga,Fernanda da Cruz Monteiro,Frederico Ozanan Barros |
author_role |
author |
author2 |
Rahal,Sheila Canevese Lima-Neto,João Ferreira Landim-Alvarenga,Fernanda da Cruz Monteiro,Frederico Ozanan Barros |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Pereira-Junior,Oduvaldo Câmara Marques Rahal,Sheila Canevese Lima-Neto,João Ferreira Landim-Alvarenga,Fernanda da Cruz Monteiro,Frederico Ozanan Barros |
dc.subject.por.fl_str_mv |
Hydroxyapatites Bone Matrix Castor Oil Polyurethanes Stem Cells |
topic |
Hydroxyapatites Bone Matrix Castor Oil Polyurethanes Stem Cells |
description |
PURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker. RESULTS: The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane. CONCLUSION: All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000500006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000500006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502013000500006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.28 n.5 2013 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126441759178752 |