Differentiation of mouse embryonic stem cells and their hybrids during embryoid body formation

Detalhes bibliográficos
Autor(a) principal: Mittmann,Josane
Data de Publicação: 2002
Outros Autores: Kerkis,Irina, Kawashima,Cinthia, Sukoyan,Marina, Santos,Enrico, Kerkis,Alexandre
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100019
Resumo: We studied the karyotypes of three hybrid clones of mouse embryonic stem cells and murine splenocytes (two having near diploid and one having near tetraploid chromosome numbers) and the characteristics of their differentiation during the formation of embryoid bodies. The X chromosome originating from embryonic stem cells may be lost in hybrids with a near diploid chromosome number and reprogramming of the "somatic" X may occur. The morphological data we obtained using light and electron microscopy revealed a correlation between the karyotype constitution of hybrid cells and their differentiation during the formation of embryoid bodies. At the beginning of development, the embryoid bodies derived from hybrid cells already showed an advanced degree of differentiation. The production of significant quantities of cartilage was typical for hybrid cells with near tetraploid chromosome numbers. The hybrid cells showed restricted pluripotent capacity and were already committed when they started to differentiate into embryoid bodies.
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spelling Differentiation of mouse embryonic stem cells and their hybrids during embryoid body formationmouse ES-somatic cell hybridskaryotypepluriopotencydifferentiationembryoid bodiesWe studied the karyotypes of three hybrid clones of mouse embryonic stem cells and murine splenocytes (two having near diploid and one having near tetraploid chromosome numbers) and the characteristics of their differentiation during the formation of embryoid bodies. The X chromosome originating from embryonic stem cells may be lost in hybrids with a near diploid chromosome number and reprogramming of the "somatic" X may occur. The morphological data we obtained using light and electron microscopy revealed a correlation between the karyotype constitution of hybrid cells and their differentiation during the formation of embryoid bodies. At the beginning of development, the embryoid bodies derived from hybrid cells already showed an advanced degree of differentiation. The production of significant quantities of cartilage was typical for hybrid cells with near tetraploid chromosome numbers. The hybrid cells showed restricted pluripotent capacity and were already committed when they started to differentiate into embryoid bodies.Sociedade Brasileira de Genética2002-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100019Genetics and Molecular Biology v.25 n.1 2002reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572002000100019info:eu-repo/semantics/openAccessMittmann,JosaneKerkis,IrinaKawashima,CinthiaSukoyan,MarinaSantos,EnricoKerkis,Alexandreeng2002-08-02T00:00:00Zoai:scielo:S1415-47572002000100019Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2002-08-02T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Differentiation of mouse embryonic stem cells and their hybrids during embryoid body formation
title Differentiation of mouse embryonic stem cells and their hybrids during embryoid body formation
spellingShingle Differentiation of mouse embryonic stem cells and their hybrids during embryoid body formation
Mittmann,Josane
mouse ES-somatic cell hybrids
karyotype
pluriopotency
differentiation
embryoid bodies
title_short Differentiation of mouse embryonic stem cells and their hybrids during embryoid body formation
title_full Differentiation of mouse embryonic stem cells and their hybrids during embryoid body formation
title_fullStr Differentiation of mouse embryonic stem cells and their hybrids during embryoid body formation
title_full_unstemmed Differentiation of mouse embryonic stem cells and their hybrids during embryoid body formation
title_sort Differentiation of mouse embryonic stem cells and their hybrids during embryoid body formation
author Mittmann,Josane
author_facet Mittmann,Josane
Kerkis,Irina
Kawashima,Cinthia
Sukoyan,Marina
Santos,Enrico
Kerkis,Alexandre
author_role author
author2 Kerkis,Irina
Kawashima,Cinthia
Sukoyan,Marina
Santos,Enrico
Kerkis,Alexandre
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Mittmann,Josane
Kerkis,Irina
Kawashima,Cinthia
Sukoyan,Marina
Santos,Enrico
Kerkis,Alexandre
dc.subject.por.fl_str_mv mouse ES-somatic cell hybrids
karyotype
pluriopotency
differentiation
embryoid bodies
topic mouse ES-somatic cell hybrids
karyotype
pluriopotency
differentiation
embryoid bodies
description We studied the karyotypes of three hybrid clones of mouse embryonic stem cells and murine splenocytes (two having near diploid and one having near tetraploid chromosome numbers) and the characteristics of their differentiation during the formation of embryoid bodies. The X chromosome originating from embryonic stem cells may be lost in hybrids with a near diploid chromosome number and reprogramming of the "somatic" X may occur. The morphological data we obtained using light and electron microscopy revealed a correlation between the karyotype constitution of hybrid cells and their differentiation during the formation of embryoid bodies. At the beginning of development, the embryoid bodies derived from hybrid cells already showed an advanced degree of differentiation. The production of significant quantities of cartilage was typical for hybrid cells with near tetraploid chromosome numbers. The hybrid cells showed restricted pluripotent capacity and were already committed when they started to differentiate into embryoid bodies.
publishDate 2002
dc.date.none.fl_str_mv 2002-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572002000100019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.25 n.1 2002
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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