GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN

Detalhes bibliográficos
Autor(a) principal: Hirai Nascimento, Priscilla Carneiro [UNIFESP]
Data de Publicação: 2016
Outros Autores: Kobayashi, Elsa Yoko [UNIFESP], de Saboya Lenzi, Luiz Guilherme [UNIFESP], Gomes dos Santos, Joao Baptista [UNIFESP], Nader, Helena Bonciani [UNIFESP], Faloppa, Flavio [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/1413-785220162402154342
https://repositorio.unifesp.br/handle/11600/57881
Resumo: Objective: To evaluate and compare the behavior of glycosaminoglycans (GAGs) in Dupuytren disease (DD). Methods: This is an experimental study with 23 patients diagnosed with DD. Tissue collected through fasciectomy with incision type Brunner or McCash were evaluated by electrophoresis for identification of GAGs. The quantification was carried out by immunofluorescence and dosage of proteins for different types of glycosaminoglycans. The results were expressed in percentage and statistically evaluated. Results: A significant increase was observed through eletrophoresis in GAGs, as compared to the control (p<0.05). Immunofluorescence of hyaluronic acid was reduced (23 times) when compared to the control (p<0.0001). Conclusion: An increase of sulfated GAGs in Dupuytren's disease, mainly dermatan sulfate, was evident from our results, as well as a pronounced decrease of hyaluronic acid in the palmar aponeurosis from the same patients.
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spelling GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTRENDupuytren contractureGlycosaminoglycansProteoglycansFasciaObjective: To evaluate and compare the behavior of glycosaminoglycans (GAGs) in Dupuytren disease (DD). Methods: This is an experimental study with 23 patients diagnosed with DD. Tissue collected through fasciectomy with incision type Brunner or McCash were evaluated by electrophoresis for identification of GAGs. The quantification was carried out by immunofluorescence and dosage of proteins for different types of glycosaminoglycans. The results were expressed in percentage and statistically evaluated. Results: A significant increase was observed through eletrophoresis in GAGs, as compared to the control (p<0.05). Immunofluorescence of hyaluronic acid was reduced (23 times) when compared to the control (p<0.0001). Conclusion: An increase of sulfated GAGs in Dupuytren's disease, mainly dermatan sulfate, was evident from our results, as well as a pronounced decrease of hyaluronic acid in the palmar aponeurosis from the same patients.Univ Fed Sao Paulo Unifesp, Escola Paulista Med, Dept Orthoped & Traumatol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Orthoped & Traumatol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo Unifesp, Escola Paulista Med, Dept Orthoped & Traumatol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Orthoped & Traumatol, Sao Paulo, SP, BrazilWeb of ScienceAtha Comunicacao & Editora2020-08-21T17:00:08Z2020-08-21T17:00:08Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion98-101application/pdfhttp://dx.doi.org/10.1590/1413-785220162402154342Acta Ortopedica Brasileira. Sao Paulo Sp, v. 24, n. 2, p. 98-101, 2016.10.1590/1413-785220162402154342S1413-78522016000200098-en.pdf1413-7852S1413-78522016000200098https://repositorio.unifesp.br/handle/11600/57881WOS:000372326500008engActa Ortopedica BrasileiraSao Paulo Spinfo:eu-repo/semantics/openAccessHirai Nascimento, Priscilla Carneiro [UNIFESP]Kobayashi, Elsa Yoko [UNIFESP]de Saboya Lenzi, Luiz Guilherme [UNIFESP]Gomes dos Santos, Joao Baptista [UNIFESP]Nader, Helena Bonciani [UNIFESP]Faloppa, Flavio [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-01T14:42:43Zoai:repositorio.unifesp.br/:11600/57881Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-01T14:42:43Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
title GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
spellingShingle GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
Hirai Nascimento, Priscilla Carneiro [UNIFESP]
Dupuytren contracture
Glycosaminoglycans
Proteoglycans
Fascia
title_short GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
title_full GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
title_fullStr GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
title_full_unstemmed GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
title_sort GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
author Hirai Nascimento, Priscilla Carneiro [UNIFESP]
author_facet Hirai Nascimento, Priscilla Carneiro [UNIFESP]
Kobayashi, Elsa Yoko [UNIFESP]
de Saboya Lenzi, Luiz Guilherme [UNIFESP]
Gomes dos Santos, Joao Baptista [UNIFESP]
Nader, Helena Bonciani [UNIFESP]
Faloppa, Flavio [UNIFESP]
author_role author
author2 Kobayashi, Elsa Yoko [UNIFESP]
de Saboya Lenzi, Luiz Guilherme [UNIFESP]
Gomes dos Santos, Joao Baptista [UNIFESP]
Nader, Helena Bonciani [UNIFESP]
Faloppa, Flavio [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Hirai Nascimento, Priscilla Carneiro [UNIFESP]
Kobayashi, Elsa Yoko [UNIFESP]
de Saboya Lenzi, Luiz Guilherme [UNIFESP]
Gomes dos Santos, Joao Baptista [UNIFESP]
Nader, Helena Bonciani [UNIFESP]
Faloppa, Flavio [UNIFESP]
dc.subject.por.fl_str_mv Dupuytren contracture
Glycosaminoglycans
Proteoglycans
Fascia
topic Dupuytren contracture
Glycosaminoglycans
Proteoglycans
Fascia
description Objective: To evaluate and compare the behavior of glycosaminoglycans (GAGs) in Dupuytren disease (DD). Methods: This is an experimental study with 23 patients diagnosed with DD. Tissue collected through fasciectomy with incision type Brunner or McCash were evaluated by electrophoresis for identification of GAGs. The quantification was carried out by immunofluorescence and dosage of proteins for different types of glycosaminoglycans. The results were expressed in percentage and statistically evaluated. Results: A significant increase was observed through eletrophoresis in GAGs, as compared to the control (p<0.05). Immunofluorescence of hyaluronic acid was reduced (23 times) when compared to the control (p<0.0001). Conclusion: An increase of sulfated GAGs in Dupuytren's disease, mainly dermatan sulfate, was evident from our results, as well as a pronounced decrease of hyaluronic acid in the palmar aponeurosis from the same patients.
publishDate 2016
dc.date.none.fl_str_mv 2016
2020-08-21T17:00:08Z
2020-08-21T17:00:08Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1413-785220162402154342
Acta Ortopedica Brasileira. Sao Paulo Sp, v. 24, n. 2, p. 98-101, 2016.
10.1590/1413-785220162402154342
S1413-78522016000200098-en.pdf
1413-7852
S1413-78522016000200098
https://repositorio.unifesp.br/handle/11600/57881
WOS:000372326500008
url http://dx.doi.org/10.1590/1413-785220162402154342
https://repositorio.unifesp.br/handle/11600/57881
identifier_str_mv Acta Ortopedica Brasileira. Sao Paulo Sp, v. 24, n. 2, p. 98-101, 2016.
10.1590/1413-785220162402154342
S1413-78522016000200098-en.pdf
1413-7852
S1413-78522016000200098
WOS:000372326500008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Ortopedica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 98-101
application/pdf
dc.coverage.none.fl_str_mv Sao Paulo Sp
dc.publisher.none.fl_str_mv Atha Comunicacao & Editora
publisher.none.fl_str_mv Atha Comunicacao & Editora
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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