Expressão de genes candidatos à determinação sexual em Astyanax scabripinnis (Teleostei: characidae) em animais com e sem cromossomos B

Detalhes bibliográficos
Autor(a) principal: Castro, Jonathan Pena
Data de Publicação: 2019
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UFSCAR
Texto Completo: https://repositorio.ufscar.br/handle/ufscar/11121
Resumo: Fish are animals present in a wide variety of habitats and are distributed all over the world. The diversity of forms, behaviors and niches associated with the evolutionary characteristics of these animals make them important to the studies associated sex determination and sexual differentiation. Among the freshwater fish of the Neotropical region, the tetras of the genus Astyanax are one of most representatives. The A. scabripinnis species complex has been well studied about the origin, distribution and frequency of B chromosomes, being considered a model organism for evolutionary studies. Several studies with population inferences about the occurrence and frequency of B chromosomes show a seasonal variation between the sexes associated with the presence of supernumerary elements in A. scabripinnis. The hypothesis is that B chromosomes could have a possible influence on the reproductive processes of this species. Thus, the present study aimed to infer if there are differences in the levels of gene expression between specimens carrying and not carrying B chromosomes concerning some candidate genes for sex determination. The results obtained showed an over expression of the dmrt1 gene in maturing male gonads of animals with B chromosomes. For amh and foxl2 genes occurred an atypical expression in females carrying B chromosomes, where their expression patterns were more similar to those of males and the reverse to those of females that do not carry Bs. The male gonads were more conservative in relation to the expression of the amh and foxl2 genes, regardless of the maturation stage and the occurrence of the B chromosomes. These results indicate that B chromosomes are changing the profiles of the sex related genes, suggesting a possible adaptive role in extending the reproductive cycle.
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spelling Castro, Jonathan PenaArtoni, Roberto Ferreirahttp://lattes.cnpq.br/8875928930712027Hattori, Ricardo Shoheihttp://lattes.cnpq.br/5554001608187705http://lattes.cnpq.br/8334644262108533db5acf52-a2c9-415e-9ba2-8ba435ebd9442019-03-22T13:53:38Z2019-03-22T13:53:38Z2019-03-01CASTRO, Jonathan Pena. Expressão de genes candidatos à determinação sexual em Astyanax scabripinnis (Teleostei: characidae) em animais com e sem cromossomos B. 2019. Tese (Doutorado em Genética Evolutiva e Biologia Molecular) – Universidade Federal de São Carlos, São Carlos, 2019. Disponível em: https://repositorio.ufscar.br/handle/ufscar/11121.https://repositorio.ufscar.br/handle/ufscar/11121Fish are animals present in a wide variety of habitats and are distributed all over the world. The diversity of forms, behaviors and niches associated with the evolutionary characteristics of these animals make them important to the studies associated sex determination and sexual differentiation. Among the freshwater fish of the Neotropical region, the tetras of the genus Astyanax are one of most representatives. The A. scabripinnis species complex has been well studied about the origin, distribution and frequency of B chromosomes, being considered a model organism for evolutionary studies. Several studies with population inferences about the occurrence and frequency of B chromosomes show a seasonal variation between the sexes associated with the presence of supernumerary elements in A. scabripinnis. The hypothesis is that B chromosomes could have a possible influence on the reproductive processes of this species. Thus, the present study aimed to infer if there are differences in the levels of gene expression between specimens carrying and not carrying B chromosomes concerning some candidate genes for sex determination. The results obtained showed an over expression of the dmrt1 gene in maturing male gonads of animals with B chromosomes. For amh and foxl2 genes occurred an atypical expression in females carrying B chromosomes, where their expression patterns were more similar to those of males and the reverse to those of females that do not carry Bs. The male gonads were more conservative in relation to the expression of the amh and foxl2 genes, regardless of the maturation stage and the occurrence of the B chromosomes. These results indicate that B chromosomes are changing the profiles of the sex related genes, suggesting a possible adaptive role in extending the reproductive cycle.Os peixes são animais presentes em uma grande variedade de hábitats e estão distribuídos pelo mundo todo. A diversidade de formas, comportamentos e nichos, associados às características evolutivas destes animais os tornam importantes ao estudo associados à evolução da determinação e diferenciação sexual. Entre os peixes de água doce mais representativos da região Neotropical, os lambaris do gênero Astyanax ocupam destaque. O complexo de espécies A. scabripinnis é um dos mais estudados quanto à origem, distribuição e frequência de cromossomos B, sendo considerado um organismo modelo para estudos evolutivos. Vários estudos com inferências populacionais acerca da ocorrência e frequência de cromossomos B mostram haver variação sazonal entre os sexos, associada com a presença destes elementos supranumerários em A. scabripinnis. A hipótese aqui postulada é de que os cromossomos B possam ter alguma influência em relação aos processos reprodutivos dessa espécie. Assim sendo, o presente trabalho objetivou inferir se há diferenças entre os níveis de expressão gênica dos principais genes candidatos à determinação sexual, conhecidos para outros vertebrados, frente a ocorrência de cromossomos B em A. scabripinnis. Os resultados obtidos mostram superexpressão do gene dmrt1 em gônadas masculinas em maturação de animais com cromossomos B. Já para os genes amh e foxl2 os resultados mostram expressão atípica para as fêmeas que são portadoras de cromossomos B. Estas fêmeas apresentam padrões de expressão destes genes mais semelhantes ao dos machos e inverso ao das fêmeas que não carregam os cromossomos adicionais. As gônadas masculinas foram mais conservadoras em relação a expressão dos genes amh e foxl2, independente do estádio de maturação e da ocorrência de cromossomo B. A partir destes resultados conclui-se que a presença de cromossomos B, de alguma maneira, está alterando os perfis de expressão dos principais genes relacionados ao sexo, sugerindo-se um possível papel adaptativo nos animais portadores dos cromossomos B na extensão do ciclo reprodutivo.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)CAPES: Código de financiamento 001porUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Genética Evolutiva e Biologia Molecular - PPGGEvUFSCarPeixesCromossomos SupranumeráriosDiferenciação SexualGenesDmrt1Foxl2AmhFishesSupernumerary chromosomesSexual differentiationdmrt1foxl2amhCIENCIAS BIOLOGICAS::GENETICACIENCIAS BIOLOGICASCIENCIAS BIOLOGICAS::GENETICA::GENETICA ANIMALExpressão de genes candidatos à determinação sexual em Astyanax scabripinnis (Teleostei: characidae) em animais com e sem cromossomos BExpression of candidates genes for sex determination in Astyanax scabripinnis (Teleostei: characidae) in animals with and without B chromosomesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisOnline600600e139de27-6b30-42af-97e0-87e2f23bea43info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALTese Final.pdfTese Final.pdfapplication/pdf1832697https://repositorio.ufscar.br/bitstream/ufscar/11121/1/Tese%20Final.pdf27b63932d38572bd9bbaa45601fa63b8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81957https://repositorio.ufscar.br/bitstream/ufscar/11121/4/license.txtae0398b6f8b235e40ad82cba6c50031dMD54TEXTTese Final.pdf.txtTese Final.pdf.txtExtracted texttext/plain134477https://repositorio.ufscar.br/bitstream/ufscar/11121/5/Tese%20Final.pdf.txt6d7dce1afcee6b3dee963feb59565085MD55THUMBNAILTese Final.pdf.jpgTese Final.pdf.jpgIM Thumbnailimage/jpeg7922https://repositorio.ufscar.br/bitstream/ufscar/11121/6/Tese%20Final.pdf.jpg3541c3e804127788a2472f0da2930d42MD56ufscar/111212023-09-18 18:31:21.095oai:repositorio.ufscar.br:ufscar/11121TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkNvbSBhIGFwcmVzZW50YcOnw6NvIGRlc3RhIGxpY2Vuw6dhLCB2b2PDqiAobyBhdXRvciAoZXMpIG91IG8gdGl0dWxhciBkb3MgZGlyZWl0b3MgZGUgYXV0b3IpIGNvbmNlZGUgw6AgVW5pdmVyc2lkYWRlCkZlZGVyYWwgZGUgU8OjbyBDYXJsb3MgbyBkaXJlaXRvIG7Do28tZXhjbHVzaXZvIGRlIHJlcHJvZHV6aXIsICB0cmFkdXppciAoY29uZm9ybWUgZGVmaW5pZG8gYWJhaXhvKSwgZS9vdQpkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlCmVtIHF1YWxxdWVyIG1laW8sIGluY2x1aW5kbyBvcyBmb3JtYXRvcyDDoXVkaW8gb3UgdsOtZGVvLgoKVm9jw6ogY29uY29yZGEgcXVlIGEgVUZTQ2FyIHBvZGUsIHNlbSBhbHRlcmFyIG8gY29udGXDumRvLCB0cmFuc3BvciBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28KcGFyYSBxdWFscXVlciBtZWlvIG91IGZvcm1hdG8gcGFyYSBmaW5zIGRlIHByZXNlcnZhw6fDo28uCgpWb2PDqiB0YW1iw6ltIGNvbmNvcmRhIHF1ZSBhIFVGU0NhciBwb2RlIG1hbnRlciBtYWlzIGRlIHVtYSBjw7NwaWEgYSBzdWEgdGVzZSBvdQpkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcwpuZXN0YSBsaWNlbsOnYS4gVm9jw6ogdGFtYsOpbSBkZWNsYXJhIHF1ZSBvIGRlcMOzc2l0byBkYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG7Do28sIHF1ZSBzZWphIGRlIHNldQpjb25oZWNpbWVudG8sIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd1w6ltLgoKQ2FzbyBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY8OqIG7Do28gcG9zc3VpIGEgdGl0dWxhcmlkYWRlIGRvcyBkaXJlaXRvcyBhdXRvcmFpcywgdm9jw6oKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFVGU0NhcgpvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7Dp2EsIGUgcXVlIGVzc2UgbWF0ZXJpYWwgZGUgcHJvcHJpZWRhZGUgZGUgdGVyY2Vpcm9zIGVzdMOhIGNsYXJhbWVudGUKaWRlbnRpZmljYWRvIGUgcmVjb25oZWNpZG8gbm8gdGV4dG8gb3Ugbm8gY29udGXDumRvIGRhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBvcmEgZGVwb3NpdGFkYS4KCkNBU08gQSBURVNFIE9VIERJU1NFUlRBw4fDg08gT1JBIERFUE9TSVRBREEgVEVOSEEgU0lETyBSRVNVTFRBRE8gREUgVU0gUEFUUk9Dw41OSU8gT1UKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBVRlNDYXIsClZPQ8OKIERFQ0xBUkEgUVVFIFJFU1BFSVRPVSBUT0RPUyBFIFFVQUlTUVVFUiBESVJFSVRPUyBERSBSRVZJU8ODTyBDT01PClRBTULDiU0gQVMgREVNQUlTIE9CUklHQcOHw5VFUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKQSBVRlNDYXIgc2UgY29tcHJvbWV0ZSBhIGlkZW50aWZpY2FyIGNsYXJhbWVudGUgbyBzZXUgbm9tZSAocykgb3UgbyhzKSBub21lKHMpIGRvKHMpCmRldGVudG9yKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzCmNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7Dp2EuCg==Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:21Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false
dc.title.por.fl_str_mv Expressão de genes candidatos à determinação sexual em Astyanax scabripinnis (Teleostei: characidae) em animais com e sem cromossomos B
dc.title.alternative.eng.fl_str_mv Expression of candidates genes for sex determination in Astyanax scabripinnis (Teleostei: characidae) in animals with and without B chromosomes
title Expressão de genes candidatos à determinação sexual em Astyanax scabripinnis (Teleostei: characidae) em animais com e sem cromossomos B
spellingShingle Expressão de genes candidatos à determinação sexual em Astyanax scabripinnis (Teleostei: characidae) em animais com e sem cromossomos B
Castro, Jonathan Pena
Peixes
Cromossomos Supranumerários
Diferenciação Sexual
Genes
Dmrt1
Foxl2
Amh
Fishes
Supernumerary chromosomes
Sexual differentiation
dmrt1
foxl2
amh
CIENCIAS BIOLOGICAS::GENETICA
CIENCIAS BIOLOGICAS
CIENCIAS BIOLOGICAS::GENETICA::GENETICA ANIMAL
title_short Expressão de genes candidatos à determinação sexual em Astyanax scabripinnis (Teleostei: characidae) em animais com e sem cromossomos B
title_full Expressão de genes candidatos à determinação sexual em Astyanax scabripinnis (Teleostei: characidae) em animais com e sem cromossomos B
title_fullStr Expressão de genes candidatos à determinação sexual em Astyanax scabripinnis (Teleostei: characidae) em animais com e sem cromossomos B
title_full_unstemmed Expressão de genes candidatos à determinação sexual em Astyanax scabripinnis (Teleostei: characidae) em animais com e sem cromossomos B
title_sort Expressão de genes candidatos à determinação sexual em Astyanax scabripinnis (Teleostei: characidae) em animais com e sem cromossomos B
author Castro, Jonathan Pena
author_facet Castro, Jonathan Pena
author_role author
dc.contributor.authorlattes.por.fl_str_mv http://lattes.cnpq.br/8334644262108533
dc.contributor.author.fl_str_mv Castro, Jonathan Pena
dc.contributor.advisor1.fl_str_mv Artoni, Roberto Ferreira
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/8875928930712027
dc.contributor.advisor-co1.fl_str_mv Hattori, Ricardo Shohei
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/5554001608187705
dc.contributor.authorID.fl_str_mv db5acf52-a2c9-415e-9ba2-8ba435ebd944
contributor_str_mv Artoni, Roberto Ferreira
Hattori, Ricardo Shohei
dc.subject.por.fl_str_mv Peixes
Cromossomos Supranumerários
Diferenciação Sexual
Genes
Dmrt1
Foxl2
Amh
topic Peixes
Cromossomos Supranumerários
Diferenciação Sexual
Genes
Dmrt1
Foxl2
Amh
Fishes
Supernumerary chromosomes
Sexual differentiation
dmrt1
foxl2
amh
CIENCIAS BIOLOGICAS::GENETICA
CIENCIAS BIOLOGICAS
CIENCIAS BIOLOGICAS::GENETICA::GENETICA ANIMAL
dc.subject.eng.fl_str_mv Fishes
Supernumerary chromosomes
Sexual differentiation
dmrt1
foxl2
amh
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::GENETICA
CIENCIAS BIOLOGICAS
CIENCIAS BIOLOGICAS::GENETICA::GENETICA ANIMAL
description Fish are animals present in a wide variety of habitats and are distributed all over the world. The diversity of forms, behaviors and niches associated with the evolutionary characteristics of these animals make them important to the studies associated sex determination and sexual differentiation. Among the freshwater fish of the Neotropical region, the tetras of the genus Astyanax are one of most representatives. The A. scabripinnis species complex has been well studied about the origin, distribution and frequency of B chromosomes, being considered a model organism for evolutionary studies. Several studies with population inferences about the occurrence and frequency of B chromosomes show a seasonal variation between the sexes associated with the presence of supernumerary elements in A. scabripinnis. The hypothesis is that B chromosomes could have a possible influence on the reproductive processes of this species. Thus, the present study aimed to infer if there are differences in the levels of gene expression between specimens carrying and not carrying B chromosomes concerning some candidate genes for sex determination. The results obtained showed an over expression of the dmrt1 gene in maturing male gonads of animals with B chromosomes. For amh and foxl2 genes occurred an atypical expression in females carrying B chromosomes, where their expression patterns were more similar to those of males and the reverse to those of females that do not carry Bs. The male gonads were more conservative in relation to the expression of the amh and foxl2 genes, regardless of the maturation stage and the occurrence of the B chromosomes. These results indicate that B chromosomes are changing the profiles of the sex related genes, suggesting a possible adaptive role in extending the reproductive cycle.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-03-22T13:53:38Z
dc.date.available.fl_str_mv 2019-03-22T13:53:38Z
dc.date.issued.fl_str_mv 2019-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv CASTRO, Jonathan Pena. Expressão de genes candidatos à determinação sexual em Astyanax scabripinnis (Teleostei: characidae) em animais com e sem cromossomos B. 2019. Tese (Doutorado em Genética Evolutiva e Biologia Molecular) – Universidade Federal de São Carlos, São Carlos, 2019. Disponível em: https://repositorio.ufscar.br/handle/ufscar/11121.
dc.identifier.uri.fl_str_mv https://repositorio.ufscar.br/handle/ufscar/11121
identifier_str_mv CASTRO, Jonathan Pena. Expressão de genes candidatos à determinação sexual em Astyanax scabripinnis (Teleostei: characidae) em animais com e sem cromossomos B. 2019. Tese (Doutorado em Genética Evolutiva e Biologia Molecular) – Universidade Federal de São Carlos, São Carlos, 2019. Disponível em: https://repositorio.ufscar.br/handle/ufscar/11121.
url https://repositorio.ufscar.br/handle/ufscar/11121
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dc.publisher.none.fl_str_mv Universidade Federal de São Carlos
Câmpus São Carlos
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Genética Evolutiva e Biologia Molecular - PPGGEv
dc.publisher.initials.fl_str_mv UFSCar
publisher.none.fl_str_mv Universidade Federal de São Carlos
Câmpus São Carlos
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