Eucalyptus grandis and Eucalyptus microcorys Essential Oils: in vitro Modulation of Hemostasis and Genotoxic Activity

Detalhes bibliográficos
Autor(a) principal: Esposito,Mariana Araújo
Data de Publicação: 2021
Outros Autores: Cesar,Pedro Henrique Souza, Brandão,Rafaela Magalhães, Abreu,Tatiane Silva de, Marcussi,Silvana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100338
Resumo: Abstract The genus Eucalyptus present high content of essential oil (EO). This study evaluated the pharmacological properties of Eucalyptus grandis (EG) and Eucalyptus microcorys (EM) EOs. The major component in both EOs was 1,8-Cineole. Both essential oils prevented thrombus dissolution and reduced clotting, hemolysis, and genotoxicity induced by snake venoms. 50% (EM) and 73% (EG) were the greatest inhibitions obtained in the thrombolytic assay - thrombolysis induced by B. moojeni venom. Increases in clotting time were also observed, with values considered significant between 10-27 seconds. Lysis values 50% lower than the negative control were observed in both EOs. The EOs also protected fibrinogenolysis induced by snake venom. EM EO was more effective in reducing venom-induced DNA fragmentation in the comet assay, with arbitrary unit values 66.15% lower than the positive control. These oils present wide application potential considering the pharmacological properties observed in this study.
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spelling Eucalyptus grandis and Eucalyptus microcorys Essential Oils: in vitro Modulation of Hemostasis and Genotoxic Activity1,8-cineolesnake venom proteasesComet assaychemical characterizationenzyme inhibitorshemostasisAbstract The genus Eucalyptus present high content of essential oil (EO). This study evaluated the pharmacological properties of Eucalyptus grandis (EG) and Eucalyptus microcorys (EM) EOs. The major component in both EOs was 1,8-Cineole. Both essential oils prevented thrombus dissolution and reduced clotting, hemolysis, and genotoxicity induced by snake venoms. 50% (EM) and 73% (EG) were the greatest inhibitions obtained in the thrombolytic assay - thrombolysis induced by B. moojeni venom. Increases in clotting time were also observed, with values considered significant between 10-27 seconds. Lysis values 50% lower than the negative control were observed in both EOs. The EOs also protected fibrinogenolysis induced by snake venom. EM EO was more effective in reducing venom-induced DNA fragmentation in the comet assay, with arbitrary unit values 66.15% lower than the positive control. These oils present wide application potential considering the pharmacological properties observed in this study.Instituto de Tecnologia do Paraná - Tecpar2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100338Brazilian Archives of Biology and Technology v.64 2021reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2021210109info:eu-repo/semantics/openAccessEsposito,Mariana AraújoCesar,Pedro Henrique SouzaBrandão,Rafaela MagalhãesAbreu,Tatiane Silva deMarcussi,Silvanaeng2022-01-07T00:00:00Zoai:scielo:S1516-89132021000100338Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2022-01-07T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Eucalyptus grandis and Eucalyptus microcorys Essential Oils: in vitro Modulation of Hemostasis and Genotoxic Activity
title Eucalyptus grandis and Eucalyptus microcorys Essential Oils: in vitro Modulation of Hemostasis and Genotoxic Activity
spellingShingle Eucalyptus grandis and Eucalyptus microcorys Essential Oils: in vitro Modulation of Hemostasis and Genotoxic Activity
Esposito,Mariana Araújo
1,8-cineole
snake venom proteases
Comet assay
chemical characterization
enzyme inhibitors
hemostasis
title_short Eucalyptus grandis and Eucalyptus microcorys Essential Oils: in vitro Modulation of Hemostasis and Genotoxic Activity
title_full Eucalyptus grandis and Eucalyptus microcorys Essential Oils: in vitro Modulation of Hemostasis and Genotoxic Activity
title_fullStr Eucalyptus grandis and Eucalyptus microcorys Essential Oils: in vitro Modulation of Hemostasis and Genotoxic Activity
title_full_unstemmed Eucalyptus grandis and Eucalyptus microcorys Essential Oils: in vitro Modulation of Hemostasis and Genotoxic Activity
title_sort Eucalyptus grandis and Eucalyptus microcorys Essential Oils: in vitro Modulation of Hemostasis and Genotoxic Activity
author Esposito,Mariana Araújo
author_facet Esposito,Mariana Araújo
Cesar,Pedro Henrique Souza
Brandão,Rafaela Magalhães
Abreu,Tatiane Silva de
Marcussi,Silvana
author_role author
author2 Cesar,Pedro Henrique Souza
Brandão,Rafaela Magalhães
Abreu,Tatiane Silva de
Marcussi,Silvana
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Esposito,Mariana Araújo
Cesar,Pedro Henrique Souza
Brandão,Rafaela Magalhães
Abreu,Tatiane Silva de
Marcussi,Silvana
dc.subject.por.fl_str_mv 1,8-cineole
snake venom proteases
Comet assay
chemical characterization
enzyme inhibitors
hemostasis
topic 1,8-cineole
snake venom proteases
Comet assay
chemical characterization
enzyme inhibitors
hemostasis
description Abstract The genus Eucalyptus present high content of essential oil (EO). This study evaluated the pharmacological properties of Eucalyptus grandis (EG) and Eucalyptus microcorys (EM) EOs. The major component in both EOs was 1,8-Cineole. Both essential oils prevented thrombus dissolution and reduced clotting, hemolysis, and genotoxicity induced by snake venoms. 50% (EM) and 73% (EG) were the greatest inhibitions obtained in the thrombolytic assay - thrombolysis induced by B. moojeni venom. Increases in clotting time were also observed, with values considered significant between 10-27 seconds. Lysis values 50% lower than the negative control were observed in both EOs. The EOs also protected fibrinogenolysis induced by snake venom. EM EO was more effective in reducing venom-induced DNA fragmentation in the comet assay, with arbitrary unit values 66.15% lower than the positive control. These oils present wide application potential considering the pharmacological properties observed in this study.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100338
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100338
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4324-2021210109
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.64 2021
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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